Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      131 008
      Structural characterization and in vitro biological exploration of phytoconstituents isolated from a chloroform extract of Rauvolfia vomitoria (Apocynaceae) root bark from Côte d’Ivoire
      (*Laboratoire de Chimie Bio-Organique et de Substances Naturelles (LCBOSN), Université Nangui Abrogoua, Abidjan, Côte d’Ivoire;

       J. Pharmacogn. Phytochem. 12(1), 6-14 (2023). TLC silica gel layers were used to monitor the purification through column chromatography (CC) of a chloroform fraction of the methanolic root bark extract of Rauvolfia vomitoria (Apocynaceae). Mobile phases were petroleum ether – ethyl acetate 4:1 (MP1), dichloromethane – methanol 20:1 (MP2), and dichloromethane – methanol 15:1 (MP3). Visualization under UV 254 nm. Preparative TLC on thicker silica gel was performed on two subfractions: (A) with dichloromethane – methanol 100:7 for the isolation of the methyl esters of eudesmic acid and of trimethoxycinnamic acid (hRF values 35 and 28, respectively, in MP1); (B) with MP2 for the isolation of an indole alkaloid: kumujan B (= 1-carbomethoxy-β-carboline, hRF value 40 in MP2). Other indole alkaloids were isolated through CC: ajmaline, mauensine and reserpine (hRF values 35, 13 and 47, respectively, in MP3).

      Classification: 4d, 7, 9, 22, 32e
      131 074
      Planar chromatographic super-hyphenations for rapid dereplication
      Gertrud MORLOCK (Institute of Nutritional Science, Chair of Food Science, and Interdisciplinary Research Center, Justus Liebig University Giessen, 35392 Giessen, Germany,

      Phytochem. Rev. (2022). The paper discussed a prioritization approach for dereplication that focuses on the most necessary to discover as a tool to deliver experimental real-world results. The principle of planar chromatographic super-hypenations was discussed, including a workflow that combined chemistry and biology to prioritize the compounds in complex samples. The workflow consisted in 1) parallel screening and separation of multiple complex mixtures using imaging HPTLC, 2) planar multiplex bioassay for non-targeted detection of important active compounds, and 3) heart-cut elution of active zones of interest directly out of the bioautogram into orthogonal HPLC-DAD-ESI-HRMS for targeted characterization. The power of planar multiplex bioassays was described for different applications, and chances and limitations for dereplication were also discussed.

      Keywords: HPTLC review
      Classification: 4d, 4e
      131 077
      In-process quality control of wine by planar chromatography versus micro planar chomatography
      S. KIRCHERT, R. KAISER, Gertrud MORLOCK* (*Institute of Nutritional Science, Chair of Food Science, and Interdisciplinary Research Center, Justus Liebig University Giessen, 35392 Giessen, Germany,

      J. Chromatogr. A. 1588, 137-149 (2019). HPTLC of sugars (1), amino acids (2), gluconic acid (3) and glycerol (4) in 20 wine samples on silica gel with i-propanol - n-butanol - boric acid solution (200 mg/10 mL) - acetic acid 14:6:3:1 for (1), 2-butanol - ammonia solution (25 %) - pyridine - water 19:5:17:13 for (2), methanol - water 7:3 for (3) and acetonitrile - boric acid solution (200 mg/10 mL) 4:1 for (4). Detection of (3) by heating at 190 °C for 20 min, followed by densitometric evaluation at 366 nm. Further detection by dipping into: 1) diphenylamine-aniline-phosphoric acid reagent, followed by heating at 120 °C for 10 min; 2) vanillin-sulfuric acid reagent, followed by heating at 135 °C for 20 min; 3) ninhydrin reagent, followed by heating at 110 °C for 5 min; 4) bromophenol blue, followed by heating at 110 °C for 10 min. Derivatized plates were documented in white light and under UV light at 366 nm. Quantification of (4) was performed using a deuterium/tungsten lamp at 380 nm. Micro planar chromatography was performed using a device, where the HPTLC foil was covered by a thick glass plate with a hole in the center, through which the mobile phase was supplied. Further analysis by mass spectrometry. 


      Classification: 4d, 6
      131 078
      Validated screening method for 81 multiclass veterinary drug residues in food via online-coupling high-throughput planar solid-phase extraction to High-Performance Liquid Chromatography−Orbitrap Tandem Mass Spectrometry
      A. MEHL, L. HUDEL, M. BÜCKER, Gertrud MORLOCK* (*Institute of Nutritional Science, Chair of Food Science, and Interdisciplinary Research Center, Justus Liebig University Giessen, 35392 Giessen, Germany,

      J. Agric. Food. Chem. 70, 10886-10898 (2022). HPTLC of 81 veterinary drugs from 6 different groups (glucocorticoids, anthelmintics, antiparasitics, coccidiostats, nonsteroidal anti-inflammatory drugs, and antibiotics) in 4 different matrices (honey, pig muscle, cow milk, and chicken eggs) on silica gel with acetonitrile - ammonia - ethanol 13:4:3 up to 80 mm and then in the reverse direction with methanol - acetonitrile - ethanol - ammonia 6:2:1:1 up to 45 mm. After each step, the plates were automatically dried in a cold stream of air for 3 min. Detection under UV light at 254 nm and fluorescence light detection (FLD) at 366 nm. Zones were eluted from the plate using a fully automated auto TLC-LC-MS interface for further analysis by high-resolution tandem mass spectrometry. Most veterinary drugs except penicillins and cephalosporins were detected at the 5 μg/kg level in pig muscle, cow milk, and chicken eggs and 25 μg/kg level in honey.

      Classification: 4d, 28a
      131 003
      Development of a high-performance thin-layer chromatography method for the quantification of alkyl glycerolipids and alkenyl glycerolipids from shark and chimera oils and tissues
      M. PAPIN, C. GUIMARAES, B. PIERRE-AUE, D. FONTAINE, J. PARDESSUS, H. COUTHON, G. FROMONT, K. MAHÉO, A. CHANTÔME, C. VANDIER*, M. PINAULT (*Nutrition, Growth and Cancer INSERM UMR 1069, University of Tours, Tours, France;

      Marine Drugs 20(4), 270 (2022). Samples were ether glycerols (EG) purified: (A) from Chimaera monstrosa liver oil (Chimaeridae); (B) from mixed liver oil of sharks Centrophorus squamosus (Centrophoridae) and Somniosus microcephalus (Somniosidae); (C) from Macaca fascicularis hearts (Cercopithecidae); (D) from tumors obtained by grafting in mice the human melanoma cell line MDA-MB-435s, and (E) from periprostatic adipose tissue of men with prostate cancer. Reduction of (phospho)ester glycerolipids into EG and fatty alcohols was part of the purification process. Octadecyl-glycerol and octadecenyl-glycerol were used as standards of alkyl- and alkenyl-glycerols, respectively. HPTLC on silica gel previously developed with chloroform – methanol 1:1, air-dried and activated for 30 min at 110° C. Application under nitrogen stream (6 bar). Development with petroleum ether – diethyl ether – acetic acid 60:140:1. After 2 h drying at room temperature under ventilation hood, visualization by 50 s immersing into sulfuric acid (7 % in ethanol), followed by 2 h drying under air-stream, and 14 min heating at 140° C. Plates were documented under white light illumination and densitometry was performed by computered scanning of the pictures. Alkyl-glycerols (mean hRF 34, LOQ 1235 ng/band) and alkenyl-glycerols (mean hRF 44, LOQ 2352 ng/band), present in all samples (except alkenyl-glycerols in shark oil), were quantified after method validation for specificity, sensitivity, accuracy, precision and repeatability. Linearity range was 1000 ng – 7000 ng for both EG types. To confirm the band identification, samples and standards were also submitted to acidic hydrolysis before HPTLC application. In this case, the bands of alkenyl glycerols did not appear, because chlorhydric acid reacted with the vinyl ether bonds to form glycerol and aldehydes.

      Classification: 4d, 4e, 9, 11c, 32f
      130 051
      Thin layer chromatography/desorption flame-induced atmospheric pressure chemical ionization/mass spectrometry for the analysis of volatile and semi-volatile mixtures
      J. SHIEA*, H. LIN (Lin Hsing Jung), S. BHAT, C. LEE (Lee Chi Yang), M. HUANG (Huang Min Zong), V. PONNUSAMY, S. CHENG (Cheng Sy Chyi) (*Department of Chemistry, National Sun Yat-Sen University, 70 Lien-Hai Road, Kaohsiung 80424, Taiwan,

      Rapid Commun. Mass Spectrom. 36, e9409 (2022). HPTLC of nicotinamide, 2-phenylacetamide, and dibenzylamine on silica gel with toluene - acetone - methanol - ammonia 8:9:2:1. Flame-induced atmospheric pressure chemical ionization (FAPCI) was used to directly characterize chemical compounds on a glass rod and drug tablet surfaces and applied as an interface TLC and mass spectrometry (MS) for the analysis of the mixture. The LOD of nicotinamide, 2-phenylacetamide, and dibenzylamine was between 5 and 50 ng/zone. 


      Classification: 4d
      130 026
      New bakuchiol dimers from Psoraleae fructus and their inhibitory activities on nitric oxide production
      Qingxia XU, Qian LV, Lu LIU, Yingtao ZHANG*, Xiuwei YANG**
      (State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing, China; *; **

      Chinese Medicine 16, 98 (2021). Preparative TLC on silica gel for the isolation of bisbakuchiol N (a terpenophenolic) from a cyclohexane extract of Psoralea corylifolia (= Cullen corylifolia, Fabaceae) mature fruits, after fractionation on silica gel, cyclodextrane and reverse-phase columns. Mobile phase was petroleum ether – chloroform 10:1. Derivatization with sulfuric acid (10 % in ethanol – water, 19:1).

      Classification: 4d, 7, 15a, 32e
      130 029
      Efficient isolation of mycosporine-like amino acids from marine red algae by fast centrifugal partition chromatography
      M. ZWERGER, S. SCHWAIGER, M. GANZERA* (*Department of Pharmacognosy, Institute of Pharmacy, University of Innsbruck, Innsbruck, Austria;

      Marine Drugs 20(2), 106 (2022). TLC was used to monitor the fractionation of hydro-methanolic extracts of Rhodophytes: Gracilaria gracilis (Gracilariaceae) (A), Porphyra sp. (Bangiaceae) (B), Spongoclonium pastorale (Ceramiaceae / Wrangeliaceae) (C); and to assess the purity of two isolated mycosporine-like amino acids: porphyra-334 and shinorine.  TLC on silica gel with n-butanol - acetic acid - water 3:1:1. Derivatization by spraying ninhydrin reagent for the detection of peptides and amino-acids; or by spraying anisaldehyde - sulfuric acid for most phytochemicals; in both cases, followed by 5 min heating at 100 °C. Visualization under white light and at 366 nm. Porphyra-334 (hRF 28) was isolated pure from (B) and (C). Shinorine (hRF 25), isolated from (A) and (B), contained a coeluting sugar (hRF 48), which was absent after further purification on solid phase extraction, and, only when isolated from (B), a coeluting peptide or amino-acid (hRF 35), which was absent after further purification on cyclodextrane column chromatography.

      Classification: 4d, 18a, 32e