Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      128 070
      Phytochemical profiling of iridoids by high‑performance thin‑layer chromatography
      Tien DO*, R. DE VAUMAS, E. REICH (*CAMAG Laboratory, Sonnenmattstrasse 11, 4132 Muttenz, Switzerland, tien.do@camag.com)

      J. Planar Chromatogr. 34, 361-366 (2021). HPTLC of 19 iridoids, including ten iridoid glycosides (catalpol, aucubin, ajugol, hastatoside, loganin, geniposide, harpagoside, verbenalin, agnuside, nuzhenide), six secoiridoid glycosides (harpagide, sweroside, swertiamarin, gentiopicroside, oleuropein, amarogentin) and three nonglycosylated iridoids (loganic acid, genipin, valtrate) in samples of Gentiana lutea, Verbena officinalis, Olea europaea and Harpagophytum procumbens on silica gel with nine different mobile phases. Detection by spraying with anisaldehyde reagent, vanillin reagent, sulfuric acid reagent, respectively, followed by heating at 100 °C for 3 min. After derivatizing the plate with Ehrlich’s reagent, the plate was heated at 100 °C for 5 min. Digital images were recorded under UV light at 254 nm and 366 nm. The data is part of a HPTLC database under development for different families of phytochemicals.

      Classification: 9, 32e
      128 026
      Green NP-HPTLC and green RP-HPTLC methods for the determination of thymoquinone: A contrast of validation parameters and greenness assessment
      A. FOUDAH*, F. SHAKEEL, M. ALQARNI, S. ROSS, M. SALKINI, P. ALAM (Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia, a.foudah@psau.edu.sa)

      Phytochem. Anal. 3078 (2021). HPTLC of thymoquinone on silica gel with cyclohexane - ethyl acetate 9:1 (1) and on RP with ethanol - water 4:1 (2). Quantitative determination by absorbance measurement at 259 nm. The hRF value of thymoquinone was 42 for system 1 and 51 for system 2. Linearity was between 25 and 1000 ng/zone for (1) and 50 and 600 ng/zone for (2). The intermediate precision was below 1 % (n=6) for (1) and (2). The LOD and LOQ were 8 and 25 ng/zone for (1) and 17 and 50 ng/zone for (2), respectively. Recovery rate was between 99.0 % and 100.9 % for (1) and 98.4 % and 101.2 % for (2). Analytical GREEnness (AGREE) scores for the systems were predicted using the AGREE software according to the 12 principles of green analytical chemistry.

      Classification: 9
      128 045
      Eight different bioactivity profiles of 40 cinnamons by multi-imaging planar chromatography hyphenated with effect–directed assays and high-resolution mass spectrometry
      N. SUMUDU, Gertrud MORLOCK* (*Justus Liebig University Giessen, Institute of Nutritional Science, Chair of Food Science, and TransMIT Center for Effect–Directed Analysis, Heinrich–Buff–Ring 26–32,
      35392 Giessen, Germany, gertrud.morlock@uni-giessen.de)

      Food Chem. 357, 129135 (2021). HPTLC of cinnamon on silica gel with toluene - ethyl acetate - methanol 6:5:3. Nine detection modes were used: 1) white light illumination, 2) UV 366 nm, 3) UV 254 nm, and six different derivatization reagents applied by immersion: 4) primuline reagent (100 mg primuline, 20 mL water and 80 mL acetone), 5) p-anisaldehyde sulfuric acid reagent (1 mL methoxy benzaldehyde, 140 mL methanol, 16 mL acetic acid and 8 mL sulfuric acid), 6) vanillin sulfuric acid reagent (1 g vanillin, 80 mL ethanol and 0.8 mL sulfuric acid), 7) diphenylamine aniline o-phosphoric reagent (2 % each of diphenylamine and aniline in 100 mL isopropanol plus 20 mL o-phosphoric acid), 8) Fast Blue B salt reagent (100 mg Fast Blue B salt in 100 mL ethanol, 70 %) and 9) natural product reagent (1 g 2-aminoethyl diphenyl borate in 100 mL ethanol), followed by heating at 110 °C (5), 120 °C (4, 6) or 140 °C (7, 8) for 3-5 min. Effect-directed profiling was performed through eight different assays: HPTLC–Aliivibrio fischeri bioassay, HPTLC–Bacillus subtilis bioassay, HPTLC–tyrosinase inhibition assay and densitometric evaluation, HPTLC–α–glucosidase and β–glucosidase inhibition assays, HPTLC–AChE and BChE inhibition assays, HPTLC–DPPH assay. Compounds were further characterized by heated electrospray ionization high–resolution mass spectrometry (HESI–HRMS).

      Classification: 9, 11a
      127 012
      Activity-guided isolation and identification of radical scavenging components in Gao-Cha tea
      R. LU (Lu Rui Li), F. HU (Hu Feng Lin), T. XIA (Xia Tao) (*Anhui Agricultural Univ., Hefei 230036, China, xiatao62@126.com)

      J. Food. Sci. 75, 239-243 (2010). HPTLC of tea made of Camellia sinensis on silica gel with chloroform - methanol - water 13:7:1. DPPH bioautography assay by spraying with 2,2-diphenyl-1-picrylhydrazyl (1 mg/mL in ethanol) under dark conditions. Detection under UV light at 254 and 366 nm. 

      Classification: 9
      127 062
      Densitometric high‑performance thin‑layer chromatography methods for the quantification of oleuropein in Olea europaea leaves and pharmaceutical preparation utilizing normal‑ and reversed‑phase silica gel plates
      P. ALAM, M. ALANAZI, H. ZATTOUT, M. ALQARNI, M. KADER* (*Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al‑Kharj 11942, Saudi Arabia, mpharm101@hotmail.com)

      J. Planar Chromatogr. 33, 609-616 (2020). HPTLC of oleuropein in the leaves of Olea europaea on silica gel with ethyl acetate - methanol - water 16:2:1 (method 1) and on RP-18 phase with ethanol - water 11:9 (method 2). Quantitative determination by absorbance measurement at 200 nm. The hRF values for oleuropein were 47 and 78 for methods (1) and (2), respectively. Linearity was between 200 and 1400 ng/zone for both methods, respectively. Intermediate precision was below 2 % (n=6). The LOD and LOQ were 68 and 203 ng/zone for method (1) and 69 and 206 ng/zone for method (2). Recovery was between 99.7 and 98.2 % for method (1) and 99.7 and 99.3 % for method (2).

      Classification: 9
      127 065
      Development and validation of thin‑layer chromatography and high‑performance thin‑layer chromatography methods for the simultaneous determination of linagliptin and empagliflozin in their co‑formulated dosage form
      M. RIZK, A. ATTIA, H. MOHAMED*, M. ELSHAHED (*Department of Analytical Chemistry, Faculty of Pharmacy, Helwan University, Cairo, Egypt, heba.elzayady@gmail.com)

      J. Planar Chromatogr. 33, 647-661 (2020). HPTLC of empaglifozin (1) and linagliptin (2) on silica gel with chloroform - methanol - ammonia (25 %) 100:10:1. Quantitative determination by absorbance measurement at 225 nm. The hRF values for (1) and (2) were 31 and 71, respectively. Linearity was between 100 and 5000 ng/zone for (1) and 50 and 2500 ng/zone for (2), respectively. Intermediate precision was below 2 % (n=3). The LOD and LOQ were 32 and 97 ng/zone for (1) and 14 and 42 ng/zone for (2), respectively. Average recovery was 100.1 % for (1) and 99.9 % for (2). Comparison with a similar TLC method showed no significant statistical differences.

      Classification: 9, 23e
      127 020
      Qualitative and quantitative analyses of aloe‑emodin, rhein, and emodin in qi yin granules by high‑performance thin‑layer chromatography
      N. XI (Xi Na), H. LIU (Liu Honbing), S. XU (Xu Shihao), X. YAN (Yan Xuehua)* (*College of TCM, Xinjiang Medical University, Urumqi 830011, Xinjiang, China, yan19810825@sina.com)

      J. Planar Chromatogr. 33, 579-585 (2020). HPTLC of aloe-emodin (1), rhein (2) and emodin (3) on silica gel with petroleum ether - ethyl acetate - formic acid 31:10:2. Quantitative determination by absorbance measurement at 440 nm. The hRF values for (1) to (3) were 47, 36 and 25, respectively. Linearity was between 90 and 426 ng/zone for (1), 64 and 304 ng/zone for (2) and 80 and 380 ng/zone for (3). Intermediate precision was below 2 % (n=6). Average recovery was 98.6 % for (1), 99.7 % for (2) and 100.0 % for (3).

      Classification: 9
      126 020
      New hirsutinolide-type sesquiterpenoids from Vernonia cinerea inhibit nitric oxide production in LPS-stimulated RAW264.7 cells
      Li-Ming YANG KUO, Pei-Yi TSENG, Yu-Chi LIN, Chia-Ching LIAW, Li-Jie ZHANG, Keng-Chang TSAI, Zhi-Hu LIN, Hsiu-O HO*, Yao-Haur KUO (*School of Pharmacy, Taipei Medical University, Taipei, Taiwan; hsiuoho@tmu.edu.tw)

      Planta Med. 84(18), 1348-1354 (2018). A subfraction (obtained through liquid-liquid partition and column chromatography) of the ethanolic extract of whole Vernonia cinerea plants (Asteraceae, subf. Cichorioideae) was further fractioned by reverse-phase SPE (solid-phase extraction) followed by preparative TLC on silica gel layer (eluent not given). For verification, zones were detected by spraying with anisaldehyde solution with 10 % sulfuric acid, followed by heating at 100 °C. Further purification by reverse-phase HPLC allowed the isolation of 6 hirsutinolide-type sesquiterpenoids (all with a oxacyclonane forming an ether bridge), including vernolides A and B.

      Classification: 8b, 9, 15a, 32e