Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
- Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
- Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
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- Search by CBS edition: Select a CBS edition and find all related publications
Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. The saved items can be printed to PDF using the print function of your web browser.
Planta Medica 84(1), 26-33 (2018). For the monitoring of the multi-step fractionation through VLC (vacuum liquid chromatography) of the chloroform-soluble parts of a methanol – water 7:3 percolate of Hippophae rhamnoides (Eleagnaceae) fruit peels, TLC on silica gel and RP-18, detection by spraying with sulfuric acid and heating. Preparative TLC on silica gel with cyclohexane – dichloromethane – methanol 5:15:1 to isolate, from VLC subfractions, three lignans (nectandrin B, fragransin A2, saucernetindiol) that are diastereoisomers of each other.
J. Planar Chromatogr. 32, 447-451 (2019). HPTLC of Fritillaria cirrhosa on silica gel with ethyl acetate - methanol - ammonia solution - water 180:20:10:1. Bioautography by dipping into a 0.15 mg/mL solution of substrate Gly-Pro-p-nitroanilide hydrochloride in 50 % of ethanol, followed by ethanol removal in the hood and dipping into a 10 U/L DPP IV enzyme solution in TrisHCl buffer (pH 8.2, 70 mM), followed by incubation at 37°C for 40 min. Detection by dipping into a solution of 0.5 % sodium nitrite in 1.2 M hydrochloric acid, followed by drying slightly for 5 min and dipping into 0.05 % N-(1-naphthyl)ethylenediamine dihydrochloride solution. Further analysis by mass spectrometry using a TLC interface. The hRF value for the dipeptidyl peptidase IV inhibitor was 58.
J. Ethnopharmacol. 235, 361-374 (2019). HPTLC of colchicine (1) and withaferin A (2) in the polyherbal ayurvedic formulation Peedantak Vati (PV) on silica gel with chloroform -
methanol - water - formic acid 140:13:2:4. Quantitative determination by absorbance measurement at 218 nm. The hRF values for (1) and (2) were 22 and 28, respectively. LOD and LOQ were 40 and 120 ng/zone for (1) and 120 and 240 ng/zone for (2), respectively.
J. AOAC Int. 102, 726-733 (2019). HPTLC of different parts of Alpinia offcinarum on silica gel with n-hexane - ethyl acetate - acetic acid 6:1:1. Antioxidant activity analysis by spraying with methanolic 0.04 % 2,2-diphenyl-1-picrylhydrazyl (DPPH*) radical reagent, followed by densitometric detection at 535 nm. Zones were scraped from HPTLC plates and further analyzed by electrospray ionization quadrupole time-of-ﬂight tandem mass spectrometry (ESI-Q-TOF-MS/MS). The method allowed the identification of three antioxidant compounds: (5R-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone, kaempferide, and galangal with hRF values of 21, 33 and 40, respectively.
J. Planar Chromatogr. 32, 41-46 (2019). HPTLC of ayapanin in the leaves of Ayapana triplinervis on silica gel with ether - ethyl acetate 3:2. Quantitative determination by absorbance measurement at 254 nm. The hRF value of ayapanin was 56. Linearity was between 200 and 1000 ng/zone. The intermediate precision was below 0.8 % (n=6). The LOD and LOQ for ayapanin were 54 and 169 ng/zone, respectively. Recovery rate was 99.7 % for ayapanin.
J. Planar Chromatogr. 21, 83-88 (2008). TLC of (+)-catechin and (-)-epicatechin on silica gel with diisopropyl ether - formic acid 9:1 in an unsaturated twin-trough chamber. Detection by spraying with vanillin-sulfuric acid reagent followed by heating at 120 °C for 5 min. Quantitative determination by absorbance measurement at 490 nm.
CBS 107, 13-15 (2011). HPTLC of 5-hydroxymethylfurfural (HMF) in honey on silica gel, prewashed with methanol - water 6:1, with ethyl acetate. Quantitative determination by densitometry in absorbance mode at 290 nm. Optional detection by immersion in p-aminobenzoic acid reagent followed by heating at 110 °C for 5-10 min. The hRf value of HMF was 80. The calibration function was polynomial in the range of 0.8-80 ng/band whilst Michaelis Menten 2 regression was suitable for higher concentrations. The LOD of HMF in honey samples was 0.75 mg/kg (12 µL applied) and the LOQ 2.4 mg/kg. The method complies with the requirement of max. 15 mg/kg of HMF in honey. The results with this method were compared with those obtained by the spectrophotometric Winkler method and by HPLC-UV and mean differences were minor (3.3 % or 0.9 mg/kg). Complementary confirmation by HPTLC-MS online coupling. HMF zones identified under UV were eluted and analyzed by ESI-MS in full-scan and SIM mode.
by principal component analysis. J. Planar Chromatogr. 28, 274-279 (2015). DPPH-HPTLC bioautography assay of Hibiscus sabdariffa on silica gel with toluene - ethyl acetate - formic acid - methanol 30:30:8:5. Analysis by dipping into 0.05 % DPPH methanolic solution. Combination with liquid chromatography-quadrupole time-of-flight mass spectrometry enabled the rapid screening and identification of the antioxidants in Hibiscus sabdariffa.