fruits. J. Planar Chromatogr. 15, 94-100 (2002). TLC of coumarins (e.g. isoimperatorin, bergapten) on silica gel and RP-2 with cyclohexane - ethyl acetate 4:1; after the first development and drying for 1 min the plates were re-chromatographed with multicomponent mixtures of dichloromethane, ethyl acetate, n-heptane, and acetonitrile. Preparative TLC on RP-2 with methanol - water 9:1. Visualization under UV 366 nm. Densitometry at 366 nm and 329 nm.

Planta med. 68, 49-54 (2002). TLC of 3,5-dihydroxy-4-methoxyxanthone, 1,3,5,7-tetrahydroxy-8-isoprenylxanthone, 1,3,5-trihydroxy-8-isoprenylxanthone, 1,5,7-trihydroxy-8-isoprenylxanthone, 1,3,7-trihydroxy-2-isoprenylxanthone, 1,5-dihydroxyxanthone, 1,6-dihydroxy-5-methoxyxanthone, 1,3,5-trihydroxyxanthone, 1,3,6-trihydroxy-5-methoxyxanthone, 1,6-dihydroxy-3,5-dimethoxyxanthone, gentisein, 3-geranyl-2,4,6-trihydroxybenzophenone, betulinic acid, 3,4-dihydroxybenzoic acid on silica gel with chloroform - methanol 9:1 and benzene - ethyl acetate 4:1, or on silica gel RP-18 with methanol - water 7:3.

International Journal of Pharma and Bio Sciences 1(1), 2-4 (2010). TLC of methanol 50 % extracts of cut dried flowers of Calendula officinalis on silica gel with chloroform – methanol 19:1. The hRf value of quercetin was 43. Quantitative determination by densitometry at 366 nm. The method was linear in the range of 1-5 µg/band. The identity of quercetin in the sample was confirmed by comparing hRf values and UV spectra of sample and standard.

Innov. Food Sci. Emerg. Technol. 26, 490-497 (2014). HPTLC of the isoflavones (1) glycitin, (2) daidzin and (3) genistin in fermented soybean powder on silica gel with toluene - ethyl acetate - formic acid - acetic acid 2:16:2:1. Quantitative determination by absorbance measurement at 260 nm. The hRF values of (1) to (3) were 36, 44 and 58, respectively.

growing in Nilgiri hills of India. J. Planar Chromatogr. 29, 347-355 (2016). HPTLC of forskolin in the roots of Coleus forskohlii on silica gel with toluene – ethyl acetate – methanol 180:60:1. Detection by dipping into anisaldehyde ‒ sulfuric acid reagent. Quantitative determination by absorbance measurement at 545 nm. The hRF value for forskolin was 48. Linearity was between 20 and 100 ng/zone. The intermediate precisions were below 1.6 % (n=3). The LOD and LOQ were 1 and 3 ng/zone, respectively. Recoveries were between 98.3 and 101.5 %.

CBS 120, 9-11 (2018). HPTLC of oligomeric proanthocyanidins (PA), alkaloids, and anthocyanins in cocoa (fresh cocoa beans, roasted cocoa, cocoa mass and molded chocolate bars) on silica gel with ethyl formate – formic acid – water – toluene 60:8:6:3 to a migration distance of 70 mm. Detection by immersion in Fast Blue Salt B reagent (140 mg Fast Blue Salt B in 140 mL methanol, 10 mL water, and 50 mL dichloromethane), followed by drying. Evaluation under UV 254 nm and white light. Quantitative determination by absorbance measurement at 280 nm for alkaloids and 510 nm for anthocyanins and derivatized PA. Elution of target zones after derivatization with Fast Blue Salt B directly into the MS, mass spectra were recorded in positive ionization mode. In general, the HPTLC method provides good separation and quick quantification of the target substances. It was found that during fermentation and roasting the levels of the alkaloids and especially oligomeric PAs diminish.

J. Chromatogr. 370, 281-292 (1986). Investigation of 29 coumarins, furocoumarins and pyranocoumarins by TLC on silanized silica - water - organic modifier Systems. Characterization of the physicochemical properties of the compounds by their hydrophobicity parameters (Pew). Quantification of the effect of individual substituents on retention by using the group contribution parameters (ARM).

J. Chromatogr. 437, 260-264 (1988). TLC on silica with ethyl acetate - formic acid - water 6:1:1. Detection by spraying with methanolic solution containing aminoethanol diphenylborate and PEG 400. Determination by densitometry at 533 nm.