Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. The saved items can be printed to PDF using the print function of your web browser.

      124 015
      Denudatin A, a dimeric acylphloroglucinol from Hypericum denudatum presents an antinociceptive effect in mice
      H. BRIDI, G. de CARVALHO MEIRELLES, S. A. de LORETO BORDIGNON, St. M. KUZE RATES, Gilsane LINO von POSER* (*Laboratory of Pharmacognosy, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Brazil; gilsane@farmacia.ufrgs.br)

      Planta Medica 83(17), 1329-1334 (2017). Two acetone-soluble subfractions of an n-hexane maceration of Hypericum denudatum aerial parts (Hypericaceae) were submitted to repeated centrifugal planar chromatography (CPC) on silica gel using n-hexane – ethyl acetate (gradient from 100:0 to 90:10), obtaining four dimeric acylphloroglucinols (denudatin A, hyperbrasilol A, uliginosin B and isouliginosin B), which were purified by crystallisation with n-hexane – dichloromethane 9:1. From the most apolar CPC eluates, a monomer (selancin A) was isolated on preparative TLC silica gel layer by elution with n-hexane – ethyl acetate 97:3. Furthermore, all the purification steps were monitored through TLC on silica gel with n-hexane – ethyl acetate 19:1 or with n-hexane – dichloromethane 1:1. Detection under UV light after derivatization with anisaldehyde – sulfuric acid, monomeric acylphloroglucinols appeared purple, whereas dimers appeared yellow-orange.

       

       

       

      Classification: 7, 32e
      123 012
      Microscopic and phytochemical comparison of the three Leonurus species L. cardiaca, L. japonicus, and L. sibiricus
      Anna PITSCHMANN, C. WASCHULIN, C. SYKORA, S. PUREVSUREN, S. GLASL* (*Department of Pharmacognosy, University of Vienna, Vienna, Austria; sabine.glasl@univie.ac.at)

      Planta Med. 83(14/15), 1233-1241 (2017). HPTLC of standards and methanolic extracts of L. cardiaca, L. japonicus and L. sibiricus (Lamiaceae) acidified with formic acid on silica gel with ethyl acetate – acetic acid – formic acid – water 100:11:11:26, in an automatic development chamber with 48 % humidity (20 min presaturation). Detection under white and UV light, before and after immersion in 1) anisaldehyde – sulphuric acid (followed by heating) for the detection of iridoids; 2) natural product reagent A for phenylpropanoids; 3) modified Dragendorff reagent (bismuth oxynitrate 0.17 %, sulfuric acid 3.5 %, glacial acetic acid 2 %, potassium iodide 4 %) for alkaloids. Ajugoside (hRF 29) and verbascoside (hRF 53) were found in L. cardiaca and L. sibiricus, but absent in L. japonicus. The opposite was true for leonurine (hRF 52), whereas stachydrine (hRF 14) was found in the three species. This method allows to distinguish L. japonicus from the other species, which have to be distinguished from each other through morphology.

      Classification: 8, 22, 32e
      123 052
      Visualization reagent for the identification of phthalates
      A. BAJAJ*, C. JOHN, A. CHAUHAN, R. TRIPATHI (*Central Forensic Science Laboratory, Plot No 2, Sector 36 A, Dakshin Marg, Chandigarh 160036, India, atulbajaj37@gmail.com)

      J. Planar Chromatogr. 32, 55-57 (2019). TLC of dimethyl phthalate, diethyl phthalate, di-n-butyl phthalate, diisobutyl phthalate, diallyl phthalate, and bis(2-ethylhexyl) phthalate on silica gel with petroleum ether - ethyl acetate - phosphoric acid 9:1:1. Detection by spraying with 0.1 % 2,6-dichlorophenolindophenol with the instantaneous appearance of pink-colored zones.

      Classification: 3e, 9
      123 054
      A new chromogenic spray reagent for the detection and identification of oxyfluorfen herbicide in biological material by High-Performance Thin-Layer Chromatography
      A. PATIL*, K. PATIL, A. PATIL, P. KULKARNI, V. CHANDEGAONKAR, B. MORE, D. MANE (*Regional Forensic Science Laboratory, Dindori Road, Nashik 422004, Maharashtra, India, atul.patil76@gov.in)

      J. Planar Chromatogr. 32, 69-71 (2019). HPTLC of oxyfluorfen in visceral tissue on silica gel with hexane - acetone 4:1. Detection by spraying with 5 % stannous chloride solution in hydrochloric acid, followed by heating at 110 ºC for 20 min. After cooling, the plates were sprayed with freshly prepared cooled (0‒5 °C) nitrating mixture of sodium nitrite in hydrochloroc acid, followed by a solution of β-napthol in sodium hydroxide. The hRF value of oxyfluorfen was 61

      Classification: 29d
      100 011
      Evaluation of quantitative thin layer chromatography using staining reagents
      R. JOHNSSON*, G. TRÄFF, M. SUNDEN, U. ELLERVIK (*Organic Chemistry, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden)

      J. Chromatogr. A 1164 (1-2), 298-305 (2007). TLC using staining reagents is fast, versatile and sometimes the only viable method method for analyzing organic compounds without chromophores. Investigation of quantitative TLC using staining reagents in combination with modern image analysis software showed that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. Illustration of the range of detection and the errors for the different parts of the process, which are largely due to the staining process but can be diminished by measuring ratios of compounds.

      Classification: 3e
      102 005
      A general detection technique for HPTLC based on changes in fluorescence
      V.L. CEBOLLA*, Elena MATEOS, L. MEMBRADO, J. VELA (*CSIC, Instituto de Carboquimica, c/Miguel Luesma, 4, 50018 Zaragoza, Spain, vcebolla@icb.csic.es)

      CBS 99, 9-11 (2007). HPTLC of petrochemical samples on silica gel pre- or post-chromatographically impregnated by dipping in methanolic berberine (60 mg/L) or coralyne (6 or 12 mg/L) solutions. Development in horizontal developing chamber with dichloromethane (saturated hydrocarbons), n-hexane (heavy gas oil), or petroleum ether - diethyl ether - acetic acid 80:20:1 (cholesterol). Quantitative determination by fluorescence measurement of berberine at 365/>450 nm and coralyne at 410/>450 nm. Linearity for alkenes was between 50 and 1500 ng and for naphtenes between 600 and 2400 ng.

      Classification: 3e
      104 094
      A validated method for quantification of glycyrrhizin in Glycyrrhiza glabra extract by HPTLC
      A GANTAIT*, N. NEMA, A. SAHU, S. PANDIT, S. BHADRA, P. MUKHERJEE (*School of Natural Product Studies, Jadavpur University, Kolkata 700032, India)

      Abstract No. 9162, IHCB (2009). HPTLC of glycyrrhizin in methanolic (70 %) extracts of Glycyrrhiza glabra on silica gel with chloroform - methanol - water 130:72:15. Quantitative determination by absorbance measurement at 254 nm and at 420 nm after spraying with anisaldehyde reagent. The method was linear in the range of 0.8-3.8 µg/spot. The limit of detection and quantification was 0.16 and 0.52 µg/spot, respectively. Glycyrrhizin was used as bioactive marker for quality control.

      Classification: 32e
      106 091
      Simultaneous quantitation of lupeol and beta-sitosterol from the whole plant powder of Asteracantha longifolia Nees
      Sunita SHAILAJAN*, S. MENON (*Herbal Research Lab, Ramnarain Ruia College, Matunga, Mumbai, India)

      Analytical Chemistry - An Indian Journal 8(1), 77-81 (2009). An HPTLC method is reported for estimation of lupeol and beta-sitosterol in the whole plant powder of Asteracantha longifolia (Acanthaceae). The proposed method was also employed for estimation of beta-sitosterol in herbal formulation containing Asteracantha longifolia. Methanolic extracts of the sample were used for chromatography. HPTLC on silica gel with toluene - ethyl acetate - methanol 75:15:7. Derivatization with Liebermann-Burchard reagent. Densitometric quantification at 366 nm. The concentration of lupeol and beta-sitosterol was 0.162 mg/g and 0.045 mg/g, respectively in the whole plant, while the concentration of beta-sitosterol was 0.039-0.048 mg/g in the formulation. The method was suitable for the quality control of the herbal formulation.

      Classification: 15a