Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      130 141
      Two-dimensional high-performance thin-layer chromatography for the characterization of milk peptide properties and a prediction of the retention behavior – a proof-of-principle study
      M. TREBLIN, T. VON OESEN, L.-C. CLASS, G. KUHNEN, I. CLAWIN-RÄDECKER, D. MARTIN, J. FRITSCHE, S. ROHN* (*Department of Food Chemistry and Analysis, Institute of Food Technology and Food Chemistry, Technical University of Berlin, Berlin, Germany; rohn@tu-berlin.de)

      J Chromatogr A 1653, 462442 (2021). Samples were peptides obtained through tryptic hydrolysis of the 5 most abundant milk proteins: α-lactalbumin (α-LA), β-lactoglobulin (β-LG), α-, β- and κ-casein (CA). As standards, synthetic whey and pea (Pisum sativum, Fabaceae) peptides (selected based on the in silico tryptic digest of α-LA, β-LG, legumin A, and vicilin with one or zero miscleavages) were only used in the last assay for prediction of the RF values of peptides with known amino-acid (AA) sequences. Two-dimensional HPTLC on silica gel (pre-washed with methanol and activated 10 min at 100°), first with basic mobile phase sec-butanol – pyridine – ammonia – water 39:34:10:26, and (after 12h drying) in the orthogonal direction with acidic mobile phase sec-butanol – pyridine – acetic acid – water 11:8:2:5. Derivatization for peptides and proteins by immersion into fluorescamine (0.05 % in acetone); visualization under UV 254 nm and 365 nm. Computer-assisted determination of the x- and y-coordinates of the derivatized zones. Repeatability (n=8) of the 2D-HPTLC was statistically tested with the Kolmogorov-Smirnov test for normal distribution and with Dixon’s Q test for outliers. Relative standard deviation (RSD) for the RF values was 12.9 % for the first dimension (y-coordinates) and 16.5 % for the second dimension (x-coordinates). According to their higher intensity and sharpness, 15 – 20 detected zones from each protein hydrolyzate were selected, manually scraped from the derivatized layer, dissolved in formic acid solution (0.1 % in acetonitrile – water 3:2), mixed with an equal volume of matrix (dihydroxybenzoic acid 2 % in acetonitrile – water 3:7), crystallized on air on a ground steel target, before being desorbed by the laser beam of the MALDI-TOF-MS/MS (matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry). Direct hyphenation of HPTLC to MS was not performed, to avoid zone diffusion during plate coating with the matrix and to circumvent the stronger binding of polar peptides on the layer.  The MS spectra were acquired in positive reflector mode in m/z range 340 – 4000 (10 – 2500 for fragments), using an external peptide as calibration standard. Identification of 51 from the 85 selected peptides according to AA sequences was performed, using software programs allowing m/z calculation of protein fragments and estimation of cleavage sites. Correlation of the retention behaviour of the peptides with their properties (molecular weight MW, isoelectric point IEP, charges, polarity) was tested with Student’s two-sided t-test after calculation of Pearson’s correlation coefficients. The correlation was significant with IEP, percentages of anionic AA and of non-polar AA; but not with the following properties: MW, percentages of cationic AA and of uncharged polar AA. Finally, based on the correlation results, regression formulas were found to calculate the x- and y-coordinates of any known peptide from the percentage of non-polar AA (or vice-versa). The prediction power of these formulas was verified by repeating the complete 2D-HPTLC-MS experiment with the standard peptides of whey and of peas, and measuring the absolute and relative deviations between the actual x- and y-coordinates and the predicted values. The absolute deviations were higher in the lower RF zones. The average, relative RF value deviations (range 22.1 – 25.7 %) were not different between whey and pea peptides.

      Classification: 2c, 2d, 4e, 18b, 19, 32e
      130 107
      Nonnegative principal component analysis in thin layer fingerprint screening: A case of Gentiana extracts from in vitro cultures
      S. GADOWSKI, K. TOMICZAK, L. KOMSTA* (*Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Lublin, Lublin, ukasz.komsta@umlub.pl)

      J. Liq. Chromatogr. Relat. Technol. 44, 820-828 (2021). HPTLC of Gentiana extracts from in vitro cultures on silica gel with acetate - methanol - water 4:1:1 in sandwich mode. Detection under UV light at 254 nm and fluorescence at 312 nm (emission above 370 nm). Principal component analysis (PCA), hierarchical cluster analysis and the novel proposal—nonnegative PCA was performed to identify common and distinct features.

      Classification: 2d
      130 015
      Mixed-mode hydrophilic interactions/reversed-phase retention mechanism in thin-layer chromatography
      Darija OBRADOVIC*, T. KOWALSKA, D. AGBABA (*Dep. of Pharm. Chem., Univ. of Belgrade-Fac. of Pharmacy, Belgrade, Serbia, darija2207@gmail.com; darija@pharmacy.bg.ac.rs)

      J Chromatogr Sci, 60 (4), 372-386 (2022). Investigation of the dual retention mechanism in TLC taking place on three stationary phases of different polarity (C-18, silica gel and DIOL)  using binary mobile phases composed of acetonitrile as the main component and water, or methanol as a modifier. The test analytes were 12 compounds of pharmaceutical importance and considerably different chemical structure, i.e. the imidazoline and serotonin receptor ligands, and their related compounds. Determination of retention of each analyte in each investigated chromatographic system in a wide enough range of the mobile phase composition, with volume fraction of the mobile phase modifier ranging from 0.10 to 0.90. Calculation of the exact turning point values as a proof of occurrence of the reversed-phase hydrophilic interaction chromatography (HILIC/RP) retention mechanism based on the multimodal retention model. Analysis of the dual retention mode with the use of the volume fraction of the mobile phase modifier, the total polarity and the total solubility models, allowing the dual (HILIC/RP) retention mechanism for the DIOL, C-18 and silica gel stationary phase to be confirmed. The observed retention mechanism was more complicated than the dual HILIC/RP one in the case of the DIOL stationary phase and acetonitrile/methanol mobile phase.

      Classification: 2d
      108 005
      The mobile phase motion in ascending micellar thin-layer chromatography with normal-phase plates
      A.P. BOICHENKO*, I.V. MAKHNO, A.Y. RENKEVICH, L.P. LOGINOVA (*Department of Analytical Biochemistry, University Centre for Pharmacy, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands; boichenko@univer.kharkov.ua; oleksandr.boychenko@gmail.com)

      J. Planar Chromatogr. 24, 463-469 (2011). Investigation of the surface tension and viscosity of micellar mobile phases based on the cationic surfactant cetylpyridinium chloride and additives of alcohols (ethanol, 1-propanol, 1-butanol, 1-pentanol). The effect of mobile phase properties on the motion of mobile phase in ascending TLC was investigated. The applicability of a quadratic relationship between the time of mobile phase motion and the distance from the solvent entry position and front of mobile phase was validated for micellar TLC.

      Keywords:
      Classification: 2d
      61 013
      Reversed-phase ion-pair systems to predict partition coefficients of ß-carbolines by HPLC and TLC
      M.C. PIETROGRANDE, P.A. BOREA, G. LODI, C. BIGHI, (Dep. Chem., Anal. Chem. Lab., Univ. Ferrara, Via L. Borsari 46, I-44100 Ferrara, Italy)

      Chromatographia 23, 713-716 (1987). Study of a group of 17 ß-carbolines in reversed phase ion pairing TLC system in order to predict their log p values. TLC data found comparable for log p prediction in HPLC.

      Keywords:
      Classification: 2d, 23e
      66 099
      Thin-layer chromatographic method for the determination of glycosyltransferase activity
      P.A. RAM, M. FUNG, C.F. MILLETTE, D.R. ARMANT, (Dep. Ant. Cell. Biol., Itarward Med. Sch., Boston, MAO2115 USA)

      Anal. Biochem. 178, 421-426 (1989). TLC of various nucleotide sugars from both high- and low-molecular-weight sugar acceptors on PEI-cellulose with 1 mM sodium phosphate buffer (pH 8.0). Determination of glycosyltransferase activity by autoradiography. Comparison of the results with those obtained by GPC and micropartitioning filtration.

      Keywords: autoradiography
      Classification: 2d, 21a
      71 010
      The effect of the coverage density of bonded octadecyl groups on the thermodynamics of retention in RP HPTLC systems
      T. KOWALSKA*, B. WITKOWSKA-KITA, M. SAJEWICZ, (*Inst. of Chem., Silesian Univ., 9 Szkolna Str., 40-006 Katowice, Poland)

      J. Planar Chromatogr. 6, 149-153 (1993). Demonstration of a theoretical model of solute retention using RP HPTLC systems with methanol - water as binary eluent and RP-18 phases differing in coverage density.

      Keywords:
      Classification: 2d
      75 007
      Sampling characteristics of octadecylsiloxane - bonded silica particle-embedded glass fiber discs for solid-phase extraction
      M.L. MAYER, C.F. POOLE*, M.P. HENRY, (*Dept. Chem., Wayne State Univ., Detroit, MI 48202, USA)

      J. Chromatogr. 695, 267-277 (1995). Use of forced flow planar chromatography to characterize the physical and retention properties of an octadecylsiloxane-bonded particle-embedded glass fiber medium used for solid-phase extraction. Discussion of the effect of the carbon loading, intraparticle porosity, being capacity and hydrophobicity index, the interparticle porosity and specific permeability on the characteristics of the medium.

      Keywords:
      Classification: 2d