Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
- Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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J. Chromatogr. A 1568, 188-196 (2018). Application of an advantageous combination, the desorption-based direct analysis in real time mass spectrometry (DART-MS) immediately after direct bioautography (DB), i.e., in the presence of microorganisms, bioassay medium and substrate reagent. The method offers a straightforward and efficient mass spectrometric detection of bioactive analytes within the bioautogram. It discriminated microorganism cells and highly polar bioassay medium ingredients which could otherwise stress the MS system. Investigation of DB-DART-MS for bioactive compounds in cosmetics using the Bacillus subtilis and Aliivibrio fischeri bioassays for detection of Gram-positive and Gram-negative antimicrobials, respectively, and the planar yeast estrogen screen for detection of estrogen-effective compounds. Study of the influences of three different bioassay matrices on the analyte response and DB-DART-MS performance on different layers (NP and RP) on the example of parabens in hand creams. Ion suppression was enhanced with increasing culture medium complexity. The mass spectrometric quantification by DB-DART-MS at the ng-level in situ each different bioautogram was verified by comparison to HPTLC-DART-MS. The total paraben content of hand creams 1 and 2 was 0.17–0.20% and 0.30–0.34%, respectively, depending on the method used. It proved that DB-DART-MS is a reliable qantitative bioanalytical hyphenation.
J. Liq. Chromatogr. Relat. Technol. 41, 1052-1065 (2019). Review of the following topics for the period of November 1, 2016 to November 1, 2018: sample preparation for TLC pesticide analysis; lipophilicity and retention studies for the study of biological activity; new reagents for pesticide detection; HPTLC-effect directed analysis on the surface of the layer; TLC-Raman spectrometry for the analysis of thiabendazole, triazophos, and phosmet residues; TLC analysis of radiolabeled pesticides; methods for the separation, detection, and qualitative and quantitative determination of pesticide residues; determination of pesticides in commercial products and the use of TLC for pesticide degradation studies. The review highlighted the isolation, characterization, and determination of less hazardous and less toxic biopesticides from plants, bacteria, fungi, and soil as the most active application area of pesticide TLC today.
J. Liq. Chromatogr. Relat. Technol. 42, 249-257 (2019). HPTLC of methanolic extracts from the leaves of Paulownia tomentosa on silica gel with chloroform - ethyl acetate - methanol 20:3:2. HPTLC-direct bioautography by dipping into B. subtilis cell suspension, followed by incubation at 28 °C for 2 h. Then the bioautograms were dipped into an aqueous solution of the MTT vital dye (1 mg/mL (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide), followed by incubation at 28 °C for 30 min. Further analysis by using a HPLC-DAD-MS system allowed the identification of apigenin and p-coumaric acid as highly abundant antibacterial components.
J. Liq. Chromatogr. Relat. Technol. 42, 122-127 (2019). Review of the application of TLC and HPTLC for the analysis of Licorice, the dried root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflate Bat., or Glycyrrhiza glabra L. The authors described methods using HPLC combined with HPLC fingerprint for rapid identification of species as well as methodologies for the analysis of glabridin on silica gel and RP-18.
J. Liq. Chromatogr. Relat. Technol. 42, 238-248 (2019). Review of recent applications of TLC in medicinal chemistry, including the determination of lipophilicity of biologically active compounds and its influence as activity descriptors of absorption, distribution, metabolism, elimination and toxicity. Practical applications of TLC as a fast screening technique in different stages of monitoring processes were also described, including systems recently used for stability studies of selected drugs.
J. Liq. Chromatogr. Relat. Technol. 42, 266-273 (2019). HPTLC of aqueous, fermented plant preparations from Chamomilla recutita L. (1), Allium cepa L. (2), Equisetum arvense L. (3) and Hamamelis virginiana L. (4) of different harvest years on silica gel with ethyl acetate - toluene - formic acid - water 16:4:3:2. The method was combined with effect-directed analysis (EDA) and high-resolution mass spectrometry (HRMS). For α-/β-glucosidase assays, the plate was sprayed with 2 mL substrate solution (60 mg 2-naphthyl-α-D-glucopyranoside or 2-naphthyl-β-D-glucopyranoside in 50 mL ethanol), then sprayed with 1 mL sodium acetate buffer and 2 mL enzyme solution (500 units α-glucosidase), followed by incubation at 37 ºC for 10 min. Analysis of multi-potent compounds was also performed using the 2,2-diphenyl-1-picrylhydrazyl reagent and Gram-positive Bacillus subtilis assays, followed by recording of elution head-based HPTLC-ESI-HRMS spectra.
J. Liq. Chromatogr. Relat. Technol. 42, 311-316 (2019). HPTLC of equol in cattle manure with methyl t-butyl ether - cyclohexane 1:1. The plate was scanned with a Time of Flight – Direct Analysis in Real Time – Mass Spectrometry (TOF-DART-MS) system. The hRF value of equol was 71. The LOD and LOQ for equol were 2.4 µg/zone and 4.5 µg/zone, respectively.
J. Liq. Chromatogr. Relat. Technol. 32, 41-46 (2019). HPTLC of flavonoids apigenin, luteolin, chrysin, myricetin, prunin (or naringenin 7-O-glucoside), nicotiflorin (or kaempferol 3-O-rutinoside), rutin (or quercetin 3-O-rutinoside), quercetin 3-O-glucopyranoside, luteolin 7-O-glucoside, isovitexin (or apigenin-6-C-glucoside), apigenin-7-O-glucoside, naringenin, hesperetin, flavone, kaempferide, kaempferol, naringin, hesperidin, quercetin dihydrate and quercetin in Caigua (Cyclanthera pedata Scrabs) on silica gel (1) or RP-18 (2) with ethyl acetate - water - formic acid 17:3:2 for (1) or 5 % formic acid in methanol - water 7:3 for (2). Detection by heating at 110 ºC, followed by dipping into Natural product reagent for 2 min. Qualitative identification under UV light at 254 nm and 366 nm. Flavonoids were further analyzed by HPTLC–MS/(MSn).