Anal. Bioanal. Chem. https://doi.org/10.1007/s00216-023-05101-y (2024). HPTLC of monogalactosyldiacylglycerol (1), sulfoquinovosyl diacylglycerol (2), and phosphatidylglycerol (3) in microalgae strains Nannochloropsis granulata, Phaeodactylum tricornutum, Porphyridium purpureum, and Tetraselmis tetrathele on silica gel with methyl acetate - isopropanol - chloroform - methanol - 0.25 % aqueous KCl solution (acidified with glacial acetic acid) 500:500:500:200:87, n-hexane - acetone - isopropanol 16:4:1 and finally with n-hexane - diethyl ether - glacial acetic acid 70:30:1. Detection by dipping into a modified copper sulfate reagent (20 g of CuSO4 × 7 H2O in 200 mL of methanol and acidified with 8 mL of 96 % sulfuric acid and 8 mL of 85 % orthophosphoric acid) for 6 s, followed by heating at 140 °C for 30 min. Quantitative determination by absorbance measurement at 720 nm. Linearity was in the range of 100-2100 ng/zone. Intermediate precisions were below 4 % (n=3). The LOD and LOQ were in the range of 18-29 ng/zone and 63-90 ng/zone, respectively. Recovery was in the range of 93.1-108.1 %.

Pharmacogn. Mag. 19, 917-925 (2023). HPTLC profiling of Guggulutiktam Kashayam on silica gel with toluene - ethyl acetate - methanol 7:3:1. Detection by spraying with anisaldehyde sulphuric acid reagent. Qualitative identification under UV light at 254 and 366 nm. 

Pharmacogn. Mag. DOI: 10.1177/09731296231189619 (2023). HPTLC of naringenin (1), ferulic acid (2) and caffeic acid (3) in Nyctanthes arbor-tristris on silica gel with toluene - ethyl acetate - formic acid 6:4:1. Quantitative determination by absorbance measurement at 371 nm. Linearity was in the range of 100-4000 ng/zone. Intermediate precisions were below 3 % (n=3). The LOD and LOQ were 30 and 45 ng/zone for (1), 15 and 90 ng/zone for (2) and 17 and 52 ng/zone for (3), respectively. Recovery was in the range of 86.7-96.3 % for (1), 101.6-102.7 % for (2) and 100.8-101.8 % for (3).

J. Sep. Sci. 46, 2300582 (2023). HPTLC of berberine in Berberis vulgaris, Berberis
aquifolium, and Hydrastis canadensis on silica gel with n-propanol - formic acid - water 95:1:4 (1) and 90:1:9 (2). Detection of (1) by spraying with dragendorff reagent, followed by drying at 100 °C. Qualitative identification under UV light at 254 and 366 nm. The hRF values for berberine in different plants were between 44 and 49.

J. Sep. Sci. 46, 2300198 (2023). HPTLC of asphaltenes-containing petroleum materials: saturated, aromatics, resins, asphaltenes group-type composition on silica-coated quartz rods with a reverse order of the subsequent elution steps, where the solvent polarity was simultaneously reduced and the chromatogram development distance increased in the following order: dichloromethane - methanol 19:1, 3 cm; toluene, 6 cm; and n-hexane, 10 cm. After each development step, the frame was placed in an oven at 70 °C until completely dried and placed in a desiccator for 10 min. Chromatograms were obtained based on the electrometer analog signal generated during the mechanical movement of individual rods in the air-hydrogen flame of the TLC–FID analyzer. 

J. Ethnopharmacol. 320, 117404 (2024). HPTLC of lupeol and stigmasterol in the stem bark of Ficus benghalensis, Ficus glomerata, Ficus religiosa, Ficus infectoria, and Albizia lebbeck on silica gel with toluene - ethyl acetate - methanol - formic acid 38:5:5:20:1. Detection by spraying with anisaldehyde sulfuric acid. Qualitative identification under UV light at 525 nm.

J. Ethnopharmacol. 319, 117085 (2024). HPTLC of betaine in Sida cordifolia on silica gel with methanol - ammonia 3:1. Detection by dipping into modified Dragendroff’s reagent, followed by heating at 120 °C for 20 min. Qualitative analysis under UV light at 540 nm.

J. Ethnopharmacol. 320, 117387 (2024). Review of the anti-infective properties of Pluchea indica, including HPTLC methods for the analysis of molecules present in different parts of the plant, such as thalictoside.