CBS 81, 14-15 (1998). HPTLC-AMD of extracts on silica gel with 16-step gradient based on methanol containing 0.1 % acetic acid via dichloromethane to n-hexane. Quantification by absorbance measurement at 255, 260, 290 and 310 nm (multi wavelength scan). Precision is determined to be 3%.

CBS 93, 14-15 (2004). HPTLC of heterocyclic aromatic amines on silica gel WRF (prewashed with methanol by chromatography followed by drying at 120 °C for 30 min) with a 6 step gradient with diethyl ether, methanol and chloroform, with alkaline conditioning via gas phase over max. 60 mm. Quantitative determination by multi-wavelength scan in absorbance mode at 252, 262, 316 nm and fluorescence measurement at 366/>400 nm. Polynomial evaluation via peak area and height. Limit of quantitation is between 1-45 ng absolute on the plate. Online coupling with MS by use of a new extractor developed by Luftmann.

Indian Drugs 41 (3), 160-164 (2004). Combinations of ofloxacin and tinidazole (1:3) are available as tablet dosage forms. Stability indicating HPTLC of ofloxacin and tinidazole on silica gel with dioxan - ethyl acetate - toluene - acetic acid - water 5.5:5:3.2:2:2 . Quantification by absorbance measurement at 307 nm. The Rf value of ofloxacin and tinidazole was found to be 0.12 and 0.85 respectively. Recovery was 99.85 % and 99.45 % for ofloxacin and tinidazole respectively. The proposed method was quantitatively evaluated in terms of calibration of concentration range, stability, and accuracy. It can be adopted for routine analysis of the formulation.

J. Planar Chromatogr. 17, 207-212 (2004). HPTLC of malvidin 3-glucoside, cyanidin 3-glucoside, delphinidin 3-glucoside, peonidin 3-glucoside,and petunidin 3-glucoside on silica gel with ethyl acetate - formic acid - twice distilled water 17:2:3 in an unsaturated twin trough chamber. After drying detection with methanolic 2,2-diphenyl-1-picrylhydrazyl reagent. Quantitative determination by videodensitometry.

J. Planar Chromatogr. 17, 169-172 (2004). HPTLC of omeprazole and pantoprazole and their impurities omeprazole sulfone and N-methylpantoprazole on silica gel with chloroform - 2-propanol - 25 % ammonia - acetonitrile (108:12:3:40). Detection under UV light at 254 nm. Quantitation of omeprazole and omeprazole sulfone at 300 nm and of pantoprazole and N-methylpantoprazole at 295 nm in reflectance-absorbance mode. Regression coefficients (r > 0.998), recovery (90.7 - 120.0 %), and detection limits (0.025 - 0.05 %) were validated and found to be satisfactory.

J. Chinese Trad. and Herb. Drugs (Zhongcaoyao), 36 (2), 277-280 (2005). HPTLC on silica gel with toluene - ethyl acetate - water - formic acid 20:10:1:1. Detection under UV 365 nm. Identification of volatile oil by fingerprint technique, combined with microscopy and a chemical method. Quantification of aristolochic acid A by HPLC.

J. Planar Chromatogr. 18, 112-117 (2005). Description of a method for optimization of a TLC separation based on use of a genetic algorithm. The procedure was tested by optimization of the reversed-phase HPTLC separation of a mixture of six pesticides; satisfactory results were obtained. The genetic algorithm was compared with the simplex method.

J. Planar Chromatogr. 18, 167-170 (2005). TLC and HPTLC of saponins from 70 species of Acer on silica gel in a horizontal chamber with chloroform - methanol - formic acid - water 200:80:20:19. Detection by spraying with 10 % sulfuric acid in ethanol or anisaldehyde - sulfuric acid reagent, followed by heating at 110 °C for 5 min, and evaluation in visible and UV light at 366 nm. Detection also by spraying with water or blood reagent.