J. Planar Chromatogr. 29, 38-44 (2016). HPTLC of tropane alkaloids (atropine sulfate, homoatropine, L-hyoscyamine, scopolamine hydrobromide, tropine and scopolamine oxide) in Datura innoxia Mill. on silica gel with methanol – buffer pH=9 1:1. The electric field gradient for the electrochromatography was 1.2 kV/cm. Detection by spraying with Dragendorff reagent. The electrostatic field was referred to be an important factor in broadening the applicability of conventional TLC systems.

J. Planar Chromatogr. 29, 310-317 (2016). HPTLC fingerprinting of two types of German propolis on silica gel with n-hexane – ethyl acetate – glacial acetic acid 5:3:1 under acidic preconditioning. Detection and documentation under UV 366 nm. Image analysis by converting chromatogram images to numerical data sets to form a data matrix. The best preprocessing was obtained by a combination of the median filter, correlation optimized warping, standard normal variate, and mean centering/autoscaling procedure.

J. of Chromatogr. Sci. 53 (2), 338-344 (2014). Presentation of a method for the simultaneous quantification of three glycosidic isoflavones (daidzin, genistin and glycitin) in soybean (Glycine max L.) by HPTLC on silica gel with toluene – ethyl acetate – formic acid – acetic acid 2:16:2:1. The hRf values of daidzin (1), genistin (2) and glycitin (3) were 39, 51 and 32, respectively. Detection and quantification by densitometry at 260 nm. Validation in accordance with the ICH guidelines with the results for precision of ≤2.1 %, ≤0.7 % and ≤0.1 %, recovery of 95.9-106.7 %, 86.9-106.6 % and 98.5-105.6 %, LOD of 3, 19 and 4 µg/mL and LOQ of 9, 59 and 11 µg/mL for the glycosidic forms of (1), (2), and (3). The method was used for the analysis of the soybean variety Kh-09 bragg which showed high amounts of glycosidic isoflavones: 278, 598 and 109 µg/g for (1), (2), and (3). After fermentation with Bacillus subtilis, the concentration of glycosidic isoflavones significantly decreased while those of the aglycone isoflavones increased.

Pharm. Res. 33, 1587-1601 (2016). HPTLC study of the cleavability of the disulfide bond in a conjugate siRNA-S-S-PE (incubated in 10 mM glutathione at 37 °C for 4 h) on silica gel with chloroform – methanol 4:1. Detection by spraying with molybdenum blue dye for the cleaved phospholipids. This test confirmed the potential utility of this system for siRNA delivery in vivo.

J. Planar Chromatogr. 29, 140-144 (2016). HPTLC of atovaquone (1) and proguanil (2) in tablets on silica gel with toluene – methanol – glacial acetic acid 40:10:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) and (2) were 80 and 22, respectively. Linearity was in the range of 200-2000 ng/zone for (1) and 100-1000 ng/zone for (2). Intermediate precisions were below 1 %. The LOD and LOQ were 41 and 123 ng/zone for (1) and 31 and 94 ng/zone for (2), respectively. Recovery was in the range of 99.5-100.7 % for (1) and 100.1-100.8 % for (2).

J. Liq. Chromatogr. Relat. Technol. 39, 271-276 (2016). HPTLC of ziprasidone and its impurities on silica gel with toluene – methanol – glacial acetic acid 15:1:1. Quantitative determination by absorbance measurement at 250 and 320 nm. The hRF value was 42 for ziprasidone and 19, 28, 31, 58 and 70 for its impurities. LOQ of impurities was 25 ng/zone. Recovery was between 94.9 and 106.7 %. See also J. Planar Chromatogr. 29, 239-246 (2016).

J. Liq. Chromatogr. Relat. Technol. 39, 674-681 (2016). HPTLC of a group of thiouracil azo dyes on RP-18 with different mixtures of water and ethanol, isopropanol, dioxane, or tetrahydrofurane. Detection under UV light at 254 nm. Compounds lipophilicity was examined as key molecular descriptor of their potential biological activity.

J. Liq. Chromatogr. Relat. Technol. 39, 607-612 (2016). HPTLC of glucose, fructose, xylose, rhamnose, arabinose, and galacturonic acid in Adansonia digitata dry fruit pulp on diol phase with a 15-step gradient based on acetone - acetonitrile 1:1 and water mixtures for enzymatic degradation products. Monosaccharide moieties were separated on silica gel with acetonitrile - acetic acid - water 63:33:5 to a developing distance of 80 mm. Detection by dipping into 10 % sulfuric acid in ethanol. Quantitative determination by absorbance measurement at 400 nm. The hRF values for rhamnose and glucose were 69 and 52, respectively. The HPTLC metehod could rapidly analyze complex mixtures containing a broad variety of monosaccharides that overlapped in a HPLC method.