J. Planar Chromatogr. 21, 233-236 (2008). Connection of two-dimensional chromatography with multiple gradient development. 2D HPTLC of anthraquinone derivatives (1,8-dihydroxyanthraquinone, franguloemodin A, aloeemodin, rhein, frangulin A, aloin, sennoside B) on silica gel. Bandwise application at the right and left corner of the plate, then development by use of multiple gradient development [G. Matysik, Chromatographia 43, 39-41 (2004)], e.g. for step 1 hexane - dichloromethane 1:1, for step 2 (hexane - dichloromethane - ethyl acetate - 80 % formic acid 50:40:10:1) and for step 3 hexane - dichloromethane - ethyl acetate - methanol - formic acid 40:40:20:5:1. After drying the plate was developed from the left and right edge with hexane - dichloromethane - formic acid 60:40:1 for step 1 and with hexane - dichloromethane - ethyl acetate - formic acid 60:35:5:1 for step 2. Quantitative determination by absorbance measurement at 440 nm. Deteciton of anthranoids in daylight and after derivatization with 10 % potassium hydroxide in methanol.

Phytochem. Anal. 18, 209-212 (2007). Separation of extracts of Solanum diflorum and Setaria parviflora by TLC on silica gel, cellulose, and RP-18 and by HPTLC on cyano and diol phase with hexane - ethyl acetate 1:1 and n-butanol - formic acid - water 5:1:4 (upper phase). Detection after distribution of ß-glucuronidase staining solution (52.5 mg agar and 0.9 mL 0.5 % iron(III) chloride solution). After solidification of the staining solution, the TLC plate was incubated for 120 min at 37 °C and immersed in a 0.2 % solution of esculin. Autography showed enzyme inhibition zones with hRf of 14 (Solanum diflorum) and 46 (Setaria parviflora).

J. Chinese Trad. Patent Med. 30 (11), 1635-1638 (2008). TLC of Sanjin Ganmao pill extracts on silica gel with 1) chloroform - methanol 17:3; 2) chloroform - methanol - formic acid 35:5:2; 3) chloroform - methanol - formic acid 90:10:1. Detection 1) by spraying with 10 % sulfuric acid in ethanol and heating; 2) by exposure to ammonia vapor. Identification by comparison with the standard hyperin and other standards of the component drugs.

J. Planar Chromatogr. 22, 395-398 (2009). HPTLC of coenzyme Q10, cholesterol and biological extracts on silica gel with petroleum ether - diethyl ether - acetic acid 85:15:1. Detection by dipping into 5 % phosphomolybdic acid in ethanol for 10 s followed by heating for 10 min at 110 °C. Quantitative evaluation in visible light.

Abstract No. 9402, IHCB (2009). HPLC and HPTLC methods were developed for the simultaneous estimation of vasicine and vasicinone in Sida cordifolia and Sida acuta roots. HPTLC of vasicine and vasicinone on silica gel with ethyl acetate - methanol - ammonia 40:10:1. Quantitative determination by absorbance measurement at 300 nm. Linearity was 320-960 ng/spot (vasicine) and 80-400 ng/spot (vasicinone). Linearity by HPLC was 4-20 µg/mL. The HPTLC method was more suitable because of high throughput and low analysis time.

60th Indian Phamraceutical Congress PA-222 (2008). HPTLC of paracetamol, aceclofenac and rabeprazole on silica gel with ethyl acetate - methanol - glacial acetic acid 90:10:1. Quantitative determination by absorbance measurement at 275 nm. Linearity was in the range of 100-500 µg/mL, 20-100 µg/mL and 2-10 µg/mL for paracetamol, aceclofenac and rabeprazole respectively. Recovery was in the range of 98.9-100.1 % for all three compounds.

J. AOAC Int. 92, 410-418 (2009). HPTLC of agrimoniin, pedunculagin, ellagic acid, gallic acid and catechin and plant extracts on silica gel, RP-18 and amino phase in a horizontal chamber. The best resolution and selectivity were achieved with diisopropyl ether - acetone - formic acid - water 4:3:2:1, tetrahydrofuran - acetonitrile - water 3:1:6, and acetone - formic acid 3:2. Polyphenols were detected under UV light at 254 nm and in visible light after spraying with 1 % methanolic iron(III) chloride or bis-diazotized sulfanilamide and after treatment with a vanillin-hydrochloric acid reagent.

60th Indian Pharmaceutical congress PA-234 (2008). HPTLC of omeprazole on silica gel with chloroform - methanol 9:1. The hRf value was 39. Quantitative determination by absorbance measurement at 302 nm. The method was linear in the range of 5-3000 ng/spot, recovery was 99.5 %. The method was suitable for routine quality control of formulations.