J. Planar Chromatogr. 22, 133-135 (2009). HPTLC of monocrotophos on silica gel with chloroform - acetone 7:3 with chamber saturation. Detection by spraying with 20 % sodium carbonate followed by acidic methanolic iron chloride reagent. Evaluation of purple spots, while organophosphorus, organochlorine, carbamate, and pyrethroid insecticides were not detected as purple spots. Alkaline hydrolysis of monocrotophos yields one molecule each of O,O-dimethylphosphoric acid and N-methylacetoacetamide. After acidiification N-methylacetoacetamide yields the enol form of monomethylamide which reacts with ferric ions to a purple complex. The detection limit for monocrotophos is 0.5 µg/zone.

J. Planar Chromatogr. 21, 343-348 (2008). HPTLC of 12 psychotropic drugs (chloropromazine, perazine, flupentixol, haloperidol, risperidon, alprazolam, midazolam, clomipramine, amitryptyline, doxepin, moclobemide, carbamazepine) on RP-18 in a horizontal chamber. The 10 mobile phases were prepared by mixing different amounts of water and the polar modifiers methanol, dioxane, acetone, acetonitrile, or tetrahydrofuran. Ammonia solution, acetate buffer (pH 4.75), or dodecyl sulfate was added to each of these mixtures. Detection under UV 254 nm.

Rev. Fac. Agron. 21, 155-160 (2004). HPTLC of betaxantin and betacyanin in the roots of Beta vulgaris on cellulose in two one-dimensional developments with 1) isopropanol – ethanol – water – acetic acid 6:7:6:1 and 2) isopropanol – ethanol – water – acetic acid 11:4:4:1. Qualitative identification under UV light. Betaxantin and betacyanin showed maximum absorbances at 537 and 465 nm, respectively. The hRf values of betaxantin and betacyanin were 22 and 34, respectively.

Indian J. Pharma. Sci. 70(6), 831-834 (2008). HPTLC of sumatriptan on silica gel (pre-washed) with methanol - water - glacial acetic acid 40:80:1 with chamber saturation for 10 min. Quantitative determination by absorbance measurement at 230 nm. The hRf value was 64. The method was linear in the range of 200-800 ng/spot.

J. Chinese Trad. Patent Med. 30 (10), 1475-1478 (2008). TLC of Baijian Cichuang suppository extracts on silica gel with 1) n-butanol - glacial acetic acid - water 7:1:2; 2) chloroform - ethyl acetate - formic acid 6:4:1; 3) petroleum ether (60-90 ºC) - diethyl ether - toluene 14:4:3. Detection 1) under UV 365 nm; 2) by spraying with 5 % vanillin in sulfuric acid, followed by heating. Identification by comparison with the standard berberine chloride and other standards of the component drugs.

Abstract No. F-282, 61st (2009). HPTLC of lipids on silica gel in a saturated twin trough chamber with carbon tetrachloride - methanol - acetic acid 270:30:11 at room temperature (25 °C). Quantitative determination by fluorescence measurement at 366 nm. The hRf value of cholesterol was 35. The calibration curve showed good linear relationship with r2=0.999 in the range of 100-600 ng/spot (via peak area).

J. Chromatogr. B 877 (29), 3719-3723 (2009). HPTLC of gemifloxacin mesylate in human plasma, extracted with chloroform - acetic acid 59:1, on silica gel with ethyl acetate – methanol - ammonia 8:4:3. The hRf value of gemifloxacin mesylate was 33. Quantification by densitometry at 254 nm. The calibration curve was established in the range of 50 to 600 ng/spot. Recovery of gemifloxacin mesylate was between 80.0 and 86.2 %. The stability of gemifloxacin mesylate in plasma was confirmed with samples submitted to three cycles of freeze–thawing at -20 °C, and with samples stored on the bench for 12 h.

Abstract No. F-347, 61st IPC (2009). HPTLC of gatifloxacin and ornidazole on silica gel with dichloromethane - methanol - 25 % ammonia 95:10:3. The hRf value was 16 and 60 for gatifloxacin and ornidazole, respectively. Quantitative determination by absorbance measurement at 302 nm. The method was linear in the range of 20-200 ng/band for gatifloxacin and 50-500 ng/band for ornidazole. Recovery was between 100.4 and 101.9 for both drugs.