CBS 104, 2-4 (2010). HPTLC of beta-boswellic acid (BA), acetyl-beta-boswellic acid (ABA), 11-keto-beta-boswellic acid (KBA), and acetyl-11-keto-beta-boswellic acid (AKBA) in Boswellia serrata extracts on silica gel with n-hexane - ethyl acetate - glacial acetic acid 16:5:1 in a twin-trough chamber saturated for 15 min. Quantitative determination by absorption measurement at 254 nm for KBA and AKBA, and at 560 nm for BA and ABA after derivatization by manual dipping in anisaldehyde reagent follwed by heating at 110 °C for 5 min. Results were compared with results from the HPLC analysis. By HPLC (injection volume 20 µL) the limits of detection for KBA and AKBA were 6-8 ng, and for BA and ABA 60-80 ng. By HPTLC (application volume 2 µL) the limits of detection for KBA and AKBA were 150 ng, and for BA and ABA 100 ng. Precision (% RSD of boswellic acids) by HPLC was between 6 and 18 % and by HPTLC below 2 % for AKBA and KBA, and around 10 % for BA and ABA after derivatization. Comparison of quantification of boswellic acid in extracts by either HPLC or HPTLC showed that both methods gave identical results. HPTLC is the method of choice for routine analysis because of lower solvent consumption and fast analysis time.

Encyclopedia of Chromatography Third Edition 1, 234-237 (2009). The author describes the combination of TLC or HPTLC with bioluminiscence detection for the effect-directed analysis (EDA) of bioactive zones on the layer. Specifically, bioactivity screening of complex mixtures by separation on HPTLC plates followed by dipping into a solution of the bioluminescent bacteria Vibrio fischeri is described.

Der Pharma Chemica 2(5), 126-132 (2010). HPTLC of drotaverine hydrochloride (DRO) and diclofenac potassium (DFK) in bulk and pharmaceutical dosage form on silica gel with toluene - ethyl acetate - methanol 1:4:1. Densitometric evaluation at at 298 nm. The hRf value was 28 ± 5 for DRO and 51 ± 5 for DFK. The linearity was in the range of 160-1280 ng/band and 100-800 ng/band. The recovery was in the range of 99.8-101.2 % for DRO and 98.3-101.4 % for DFK.

Biochem. Biophys. Res. Commun. 399, 716-720 (2010). HPTLC of globotriaosylceramide (Gb3) in mouse tissues on silica gel with chloroform - methanol - water 65:25:4. Detection by HPTLC-immunostaining with an anti-Gb3 monoclonal antibody. Quantification by densitometry with a luminescent image analyzer. The limit of detection of Gb3 was 50 ng/zone.

Analytical Science 25(1), 57-62 (2009). HPTLC of minocycline on silica gel plates previously treated with 10 % EDTA solution of pH 9.0 with methanol - acetonitrile - isopropyl alcohol - water 10:8:1:1. The hRf value was 32. Densitometric evaluation at 345 nm. The method was linear in the range of 100-1200 ng/band for all biological samples. The average recovery was 95.1 %. The method was found suitable for estimation of the drug in different biological fluids (human plasma, saliva, gingival fluid) and can be used for pharmacokinetic studies.

J. Liq. Chromatogr. Relat. Technol. 33, 957-971 (2010). HPTLC of pyritinol on silica gel with dichloromethane - methanol - formic acid 9:1:1 in a twin trough chamber. Quantitative determination by absorbance measurement at 300 nm. Repeatability and intermediate precision in matrix were 0.4 % and 3.0 %, respectively. Recoveries of spiked samples at three levels ranged from 98.5 to 101.9 % with intermediate precisions of RSD 3.7 to 4.7 %. The limit of detection and quantification was 0.6 and 2.0 µg/mL (6 and 20 ng/band), respectively. Selectivity was evaluated determining the peak purity by UV-spectrophotometry and showed correlation coefficients (r) > 0.9997. Additionally the selectivity was proven by mass spectrometry. Mass spectra showed only the analyte signals which indicated a highly satisfying selectivity.

J. Sep. Sci. 33, 2206-2210 (2010). HPTLC of fluoxetine on silica gel with toluene - glacial acetic acid 4:5. Derivatization of fluoxetine with 4-dimethylamino-azobenzene-4-sulfonyl chloride. Quantitative determination by absorbance measurement at 272 nm. The hRF of fluoxetine was 45. Linearity was between 0.05-0.35 ng/µL. Detection and quantification limits were 230 and 700 ng/µL, respectively. The intra-day and inter-day precisions showed a %RSD lower than 3.9 %. Recovery (by standard addition) was 94.7 %. The method was designed to cover the usual serum concentration level of patients taking the drug.

J. of Beihua Univ. (Natural Sci.), 11 (5), 420-423 (2010). TLC on silica gel with 1) petroleum ether (60-90 ºC) - ethyl acetate 4:1 and 2) chloroform - propanone - n-hexane - acetic acid 80:40:2:5. Detection under UV 365 nm. Derivatization by spraying with 10 % vanillin in sulfuric acid and heating at 105 ºC until the zones were visualized. Identification by comparison with the standards of the components in the individual composition drug.