Asian J. Chem. 19(6), 4183-4187 (2007). HPTLC of atenolol and indapamide in tablet formulation on silica gel with toluene - ethanol - acetone - acetic acid 70:25:30:3. Quantitative determination by absorbance measurement at 266 nm. The hRf value of atenolol and indapamide was 21 and 74, respectively. The linearity range was 3.8-10.9 ng/spot and 0.2-0.6 ng/spot for atenolol and indapamide respectively. The recovery was in the range of 98.7-100.1 % for both compounds.

60th Indian Pharmaceutical Congress PA-200, (2008). HPTLC of fluoxetine HCl and olanzapine on silica gel with acetone - methanol - triethylamine 10:6:1. Quantitative determination by absorbance measurement at 235 nm. The method was linear in the range of 300-1000 ng/spot for fluoxetine HCl and 50-500 ng/spot for olanzapine respectively. The method was suitable for quality control of combined dosage form.

Asian J. Chem. 19(5), 3627-3632 (2007). TLC of simvastatin and ezetimibe on silica gel with ethyl acetate - chloroform 4:1. Quantitative determination by absorbance measurement at 220 nm. The hRf value of simvastatin was 76 and of ezetimibe 89. Linearity was between 600 and 1400 µg/mL for simvastatin and ezetimibe. The recovery (by standard addition method) was in the range of 99.7 and 99.6 % for both drugs. The proposed method is precise, accurate and can be used for routine analysis of simvastatin and ezetimibe in tablets.

Anal. Chem. 75, 118-125 (2003). Online TLC separation and electrospray mass spectrometry (TLC/ESI-MS) by direct linking of a commercial overpressure TLC instrument, OPLC 50, and a Q-TOF mass spectrometer. Separation on silica gel with dichlormethane - methanol - water 60:35:8. A sensitivity of 5 pmol of glycosphingolipid was readily demonstrated for TLC/ESI-MS and 20 pmol for TLC/ESI-MS/MS production scanning to derive the saccharide sequence and long chain base/fatty acid composition of the ceramide. Initial preconditioning of TLC plates is necessary to achieve high sensitivity detection by reducing chemical background noise. Plates can be used repeatedly (at least 10 times) for analysis, although this may result in a minor reduction in TLC resolution. Following solvent development, separated components on the TLC plates can be detected in the conventional way by nondestructive staining or UV absorption or fluorescence and can be stored for on-line TLC/ESI-MS analysis at a later stage without reduction in mass spectrometric detection sensitivity and chromatographic resolution. Aspects for further improvement of OPLC instrumentation include use of narrower TLC plate dimensions and refined design of the eluate exit system.

The effect of a complex biological matrix on quantitative performance. J. Planar Chromatogr. 22, 9-14 (2009). HPTLC-OPLC of fructose, glucose, galactose, sucrose, lactose, 1-kestose, raffinose, nystose, and fructosil-nystose on silica gel with acetonitrile - water 17:3. After development and drying, the separated bands were derivatized by a thermal in-situ reaction on a hot plate. The plates were immersed in lead(IV) acetate-dichlorofluorescein reagent for 9 s and heated at 105 °C for 3 min. Quantitative determination by fluorescence measurement at 313 nm.

J. Agric. Food Chem. 56, 8050-8057 (2008). HPTLC of [2-14C]-cymoxanil in the cultures (medium and mycelium) and in the cell-free extracts of Botrytis cinerea on silica gel with hexane - ethyl acetate - acetic acid 70:30:1. The most polar products were separated on RP-18 (impregnated with a methanolic solution of tetrabutylammonium bromide 70 mM and dried at 80 °C for 5 min) with phosphate buffer (0.01 M, pH 6) – methanol 11:9. Detection by autoradiography. The hRf value of cymoxanil was 35 on silica gel. Different metabolites were detected using both stationary phases.

Indian Drugs 46(2), 150-153 (2009). HPTLC of hydrochlorthiazide (HZ) in combination with quinapril (QP) or candensartan (CD) on silica gel with toluene - ethyl acetate - glacial acetic acid 2:12:1 (for HZ and QP), or 20:50:1 (for HZ and CD), with chamber saturation for 30 min at room temperature. Quantitative determination by absorbance measurement at 273 nm. The method was linear in the range of 480-960 (HZ), 400-800 (QP) and 50-250 ng/spot (CD).

J. Sep. Sci. 32, 1454-1458 (2009). HPTLC of carbamazepine in human serum on silica with ethyl acetate – toluene – methanol – glacial acetic acid 10:8:1:1. Detection by dipping in a solution of perchloric acid 60 % – ethanol – water 1:1:1, followed by heating at 120-150 °C for 5-10 min. Quantitative determination by fluorescence measurement at 366/>400 nm. The accuracy and precision did not exceed 3.2 % RSD at any level. The hRf value of carbamazepine was 55 and selectivity regarding matrix was given. Linearity was between 3 and 20 ng/µL. The intra-assay and inter-assay precision, expressed as the RSD, were in the range of 0.4 - 1.2 % (n = 3) and 2.2 - 3.2 % (n = 9), respectively. The limit of detection and quantification was 0.19 and 0.57 ng, respectively. Recovery (by standard addition) was between 99.0 and 102.0 %.