J. Planar Chromatogr. 21, 39-42 (2008). HPTLC of neutral lipids and ubiquinone on silica gel with petroleum ether - diethyl ether - acetic acid 80:20:1. Detection with phosphomolybdic acid reagent. Quantitative determination by densitometry at 610 nm. Specific detection reagents were used to confirm the identity of particular lipid classes. The studies confirmed the presence of free sterols, free fatty acids, methyl esters, hydrocarbons and ubiquinone, and triacylglycerols.

by HPTLC. J. Planar Chromatogr. 21, 183-186 (2008). HPTLC of dopamine on silica gel with n-butanol - acetic acid - water 7:2:1. Detection and quantification by densitometry at 280 nm.

J. AOAC Int. 91, 13-20 (2008). Comprehensive proposal for the validation of qualitative HPTLC methods. The steps of the validation process (method selection and optimization, stability, specificity, precision, and robustness) are illustrated with examples of identification methods: green tea leaf, ginseng root, eleuthero root, echinacea root, black cohosh rhizome, licorice root, kava root, milk thistle aerial parts, feverfew aerial parts, and ginger root. The validation protocol is a key element for structuring, managing and documenting the validation process. HPTLC is suitable for reliable identification of botanicals because it can provide chromatographic fingerprints that can be visualized and stored as electronic images. Reproducibility is improved if suitable instrumentation is used, a standardized HPTLC methodology is implemented, and methods have been validated.

CBS 99, 9-11 (2007). HPTLC of petrochemical samples on silica gel pre- or post-chromatographically impregnated by dipping in methanolic berberine (60 mg/L) or coralyne (6 or 12 mg/L) solutions. Development in horizontal developing chamber with dichloromethane (saturated hydrocarbons), n-hexane (heavy gas oil), or petroleum ether - diethyl ether - acetic acid 80:20:1 (cholesterol). Quantitative determination by fluorescence measurement of berberine at 365/>450 nm and coralyne at 410/>450 nm. Linearity for alkenes was between 50 and 1500 ng and for naphtenes between 600 and 2400 ng.

Ind. J. Pharm. Sci. 69 (6) 841 - 844 (2007). HPTLC of artesunate on silica gel with toluene - ethyl acetate - acetic acid 20:80:2. Detection by treatment with vanillin reagent (1 % vanillin in 5 % ethanolic sulphuric acid) leads to pink zones which are stable for more than a day. Quantitative determination by absorbance measurement at 520 nm. The hRf value for artesunate was 44. Linearity was between 100 and 600 ng per spot. Recovery (by standard addition method) was 98.9 - 99.9 % for tablets and injections.

Chromatographia 68 (9-10), 855-859 (2008). HPTLC of moclobemide on silica gel with benzene – methanol – 40 % ammonia 70:30:1. Quantification by absorbance measurement at 238 nm. The degradation products reached under acidic, basic, and oxidising conditions were well resolved from the pure drug. Linearity was in the range of 50–600 ng/band, with a determination coefficient r2 of 0.9967 ± 0.51. LOD and LOQ, determined experimentally, were 10 and 30 ng/band, respectively. The method was used to investigate the kinetics of alkaline degradation, the Arrhenius plot was constructed and the activation energy calculated.

J. AOAC Int. 91, 1210-1217 (2008). HPTLC of monocrotophos, quinalphos, triazophos, parathion-methyl, isophenphos-methyl, temephos, parathion, phoxim, and chlorpyrifos on silica gel with automated multiple development. HPTLC of phoxim and chlorpyrifos on silica gel with dichloromethane - hexane 1:1 in a twin-trough chamber. Quantitative determination by absorbance measurement at 254 nm.

60th Indian Pharmaceutical Congress PA-202 (2008). HPTLC of 6-gingerol and 3-acetyl-11-keto-beta-boswellic acid on silica gel with n-hexane - ethyl actate 7:3 in a chamber saturated at ambient temperature. Quantitative determination by absorbance measurement at 254 nm. The hRf values were 48 and 58 for 3-acetyl-11-keto-beta-boswellic acid and 6-gingerol respectively. The recovery was 98.7-100.8 % for both compounds. The chromatographic conditions were suitable for routine analysis.