J. Planar Chromatogr. 28, 386-390 (2015). HPTLC of gallic acid (1) and berberine (2) in a polyherbal formulation on silica gel with toulene – ethyl acetate – formic acid – methanol 24:18:8:1 for (1) and ethanol – water – formic acid 90:9:1 for (2). Quantitative determination by absorbance measurement at 273 and 366 nm for (1) and (2), respectively. The hRF values for (1) and (2) were 58 and 76, respectively.

J. Sep. Sci. 38, 4180-4186 (2015). HPTLC of glycyrrhizic acid in the dried root and rhizoma of the plants Glycyrrhiza uralensis Fisch, Glycyrrhiza inflata Bat, and Glycyrrhiza glabra L. on silica gel with ethyl acetate - water - acetic acid - formic acid 14:2:2:1. Detection by spraying with 10 % solution of sulfuric acid in ethanol, followed by heating at 105 °C for 10 min. Quantitative determination by absorbance measurement at 365 nm.

J. AOAC Int. 98, 1226-1233 (2015). HPTLC of three phosphodiesterase type 5 inhibitors, sildenafil (1), vardenafil (2), and tadalafil (3), and eight of their analogs, hydroxyacetildenafil (4), homosildenafil (5), thiohomosildenafil (6), acetildenafil (7), acetaminotadalafil (8), propoxyphenyl (9), hydroxyhomosildenafil (10), hydroxyhomosildenafil (11) and hydroxythiohomosildenafil (12) in finished products on silica gel with TBME - methanol - ammonia 28% 20:2:1. Detection under 254 and 366 nm UV light and by absorbance measurement at 232 nm. LOD was 30 ng/zone for all substances. Confirmation by HPTLC-ESI-MS. The method was successfully_x000D_ applied to screening of 45 commercial lifestyle products. Of those, 31 products tested positive for at least one illegal component (sildenafil, tadalafil, propoxyphenyl, hydroxyhomosildenafil, or dimethylsildenafil).

Food Chem. 210, 200-211 (2016). Review of the principles and applications of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) in food science and related fields. The review described the combination of MALDI-MSI and HPTLC, including the visualization of phospholipids in chicken egg yolk on silica gel with acetonitrile – water 1:1.

by the combination of high-performance thin-layer chromatography with direct bioautography and mass spectrometry. J. of Chromatogr. A 1422, 310-317 (2015). Investigation of two tansy (Tanacetum vulgare L.) essential oils, obtained by steam distillation of the capitula with subsequent liquid-liquid extraction (oil 1) or with use of an auxiliary phase for the trapping of the steam components (oil 2), against Bacillus subtilis F1276, B. subtilis spizizenii (DSM 618), Xanthomonas euvesicatoria, Pseudomonas syringae pv. maculicola, Ralstonia solanacearum strain GMI1000 and Aliivibrio fischeri by using the coupling of HPTLC to direct bioautography (HPTLC-DB). Oil 2 was richer in components and provided more inhibition zones due to the advanced extraction process. Identification of the main bioactive components by scanning HPTLC-direct analysis in real time mass spectrometry (HPTLC-DART-MS) and solid-phase microextraction GC electron impact MS (SPME-GC-EI-MS) as cis- and trans-chrysanthenol as well as trans-chrysanthenyl acetate. The results indicated that cis-chrysanthenol exhibited antibacterial effects against all tested bacteria. Trans-chrysanthenol inhibited B. subtilis, R. solanacearum and A. fischeri, and trans-chrysanthenyl acetate was an inhibitor for X. euvesicatoria, R. solanacearum and A. fischeri. However, the ionization characteristics and the recorded mass spectra showed that DART is a softer ionization technique than EI and more affected by ambient conditions and thus prone to additional oxidation products, although HPTLC-DART-MS resulted in a comparable fragmentation.

seed (Takhmaria). J. Planar Chromatogr. 29, 216-220 (2016). HPTLC of apigenin in the seeds of Ocimum basilicum on silica gel with toluene – acetone – formic acid 5:4:1. Quantitative determination by absorbance measurement at 340 nm. The hRF value of apigenin was 71. Linearity was in the range of 100-600 ng/zone. Intermediate precisions were below 0.5 %. The LOD and LOQ were 4 and 12 ng/zone. Recovery was between 98.5 and 100.6 %.

J. Agric. Food Chem. 64, 1245-1255 (2016). Preparative HPTLC of gangliosides in the frontal lobe of piglets on silica gel with chloroform – methanol – calcium chloride 50:42:11. Fractions were subjected to a series of reactions to obtain sialic acid, glucose, galactose, N-acetyl-galactosamine, and fatty acid derivatives.

CBS 116, 13-15 (2016). HPTLC of nicotine in e-liquids on silica gel with chamber saturation (with filter paper) for 20 min with toluene – acetone –diethylamine 10:10:1 to the migration distance of 70 mm. Detection under UV 254 nm. Quantitative determination by absorbance measurement at 260 nm. Elution of the zones with methanol (with 0.1 % formic acid) into a single quadrupole MS and detection in positive ionization mode. The hRf value of nicotine was 56 and separation from other ingredients (propylene glycol, glycerol, flavors) was good. Visual evaluation of the samples for presence of nicotine was confirmed with MS and UV spectra.