J. Ethnopharmacol. 289, 115035 (2022). HPTLC of Unani pharmacopoeial preparations Itrifal Hakim Ali (1) and Habb-e-Kalaf (2) on silica gel with toluene - ethyl acetate 9:1 for (1) and toluene - ethyl acetate 4:1. Detection under UV light at 254 and 366 nm. 

J. Ethnopharmacol. 295, 115327 (2022). Review of modern applications for the analysis of Rauvolfia species, including traditional uses, phytochemistry, quality control, pharmacological properties, as well as clinical evidence that may be useful in the drug discovery process. The paper described qualitative and quantitative methods, including HPTLC methods for the analysis of targeted and non-targeted compounds in different extracts of plant parts of Rauvolfia species.

J. Ethnopharmacol. 289, 115035 (2022). HPTLC of stigmasterol and some polyphenolic compounds in the rhizome of Cyperus tegetum on silica gel with chloroform - ethyl acetate - formic acid 5:4:1 and toluene - methanol 9:1. Detection by spraying with p-anisaldehyde reagent in a cold solution of methanol - glacial acetic acid - phuric acid 17:2:1, followed by heating at 100 - 105 °C for 5-10 min.

J. Ethnopharmacol. 305, 116004 (2023). Review of the ethnobotany, phytochemistry, and biological activities of the medicinally important Prunus africana. The paper described various TLC and HPTLC methods to isolate and analyse P. africana extracts, including the identification of myristic acid, palmitic acid, linoleic acid, oleic acid, stearic acid, arachidonic acid, n-docosonal, behenic acid, lignoceric acid, β-sitosterol, and ursolic acid.

Phytochem. Anal. 33, 564-576 (2022). HPTLC of flavonoids (1), anthocyanin (2) and antioxidant structures in sweet cherry (Prunus avium) on silica gel with ethyl acetate - dichloromethane - formic acid - acetic acid - water 100:25:10:10:11 for (1) and ethyl acetate - formic acid - acetic acid - water 100:11:11:26 for (2). Detection by spraying with natural product reagent/polyethylene glycol 400 (NP/PEG) solution or 0.1 % methanolic DPPH solution. Qualitative analysis under UV light at 366 nm. 

Phytochem. Anal. 33, 1177-1189 (2022). HPTLC of 35 species from four genera
(Combretum, Pteleopsis, Quisqualis, Terminalia) of Combretaceae on silica gel with ethyl acetate - formic acid - water 50:3:3. Detection by spraying with 10 % methanolic sulfuric acid and visualization under white light. Detection of flavonoids by spraying with Natural Product Reagent and examined under UV light at 366 nm. For bioautography analysis, the developed plate was sterilized under UV light for 1 h, then overlaid with tryptone soya agar containing the appropriate bacterial culture: Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 11778), Escherichia coli (ATCC 8739), and Salmonella typhimurium (ATCC 14028). Active compounds were identified by mass spectrometry. 

Phytochem. Anal. 34, 5-29 (2023). Review of the plant resources, chemical ingredients, and biological activities of Euodiae fructus, focusing on the chromatographic and mass spectrometric technologies used for analysis of Euodiae fructus. The paper described TLC and HPTLC methods for the analysis of different analytes such as rutaecarpine and evodiamine and their application in traditional chinese medicine research. 

Phytochem. Anal. 33, 1018-1027 (2022). HPTLC of an α-amylase inhibitor from the aerial part of Asparagus racemosus Willd on silica gel with n-hexane - ethyl acetate - acetic acid 20:10:1. Detection of free-radicals scavenging active compounds by spraying with 0.2 % DPPH• solution in methanol, followed by incubation for 30 min in the dark at room temperature. The antioxidant activity was measured by adding the area of the bright yellow zones and expressing it to gallic acid equivalents (GAEs), using gallic acid (1–15 μg/μL) to construct a calibration curve as the model analyte. Biochemical analysis by dipping into freshly prepared α-amylase solution, followed by incubation at 37 °C for 40 min in a humid chamber. After incubation, the plate was immersed in the substrate solution (2 % starch solution), followed by incubation for another 15 min to allow the enzyme-substrate reaction to occur. Detection by spraying with Gram's iodine solution. By comparing the area of blue bands obtained by samples and standard acarbose the inhibitory activity was measured. Linearity was in the range of 1-7 μg/zone for acarbose and 1-15 μg/zone for gallic acid. The LOD and LOQ were 3 and 9 μg for both acarbose and gallic acid. Further analysis by total reflectance-Fourier-transform infrared (ATR-FTIR) and nuclear magnetic resonance (NMR) spectroscopy.