Indian Drugs 43 (12), 989-982 (2006). HPTLC of lupeol in a methanolic extract of powdered Vernonia cinerea L. on silica gel with dichloromethane - toluene - acetone - methanol 30:50:5:3. Detection by spraying with anisaldehyde reagent. Quantitative determination by absorbance measurement at 581 nm. The concentration of lupeol in Vernonia cinerea L. was found to be 2.01 µg. The method was validated and can be used for routine quality control of Vernonia cinerea L. including quantitation of lupeol.

CBS 97, 2-5 (2006). HPTLC of gangliosides on silica gel with chloroform - methanol - water 24:17:4 and addition of 2 mM CaCl2, after chamber saturation with filter paper for 3 h, over 80 mm, followed by drying for 5 min at room temperature. Detection by dipping in orcin solution (0.3 % (w/v) in 3 M H2SO4) followed by heating at 100 °C for 3 min. Alternative detection of GM3-bands by derivatization with primulin (0.02 % (w/v) in aceton - water 4:1). Quantitative determination by direct IR-MALDI-o-TOF-analysis. The limit of detection for GM3 was about 50 ng/zone.

Food Chem. 91, 757-764 (2005). HPTLC of dihydrosanguinarine (1), after its conversion to sanguinarine (2) as an index of argemone oil adulteration in edible mustard oil, on silica gel with hexane – acetone – methanol 16:3:1. The plate was irradiated under long wave UV light for 15 min to oxidize (1) to (2). Quantitative determination by absorbance measurement at 366 nm. The hRf values for (1) and (2) were 82 and 36, respectively. Linearity was between 5 and 300 ng/zone for (2). The limit of detection and quantification was 1 and 3 ng/zone. Recovery was between 79 and 82 %.

Indian Drugs 44(10), 734 (2007). HPTLC of valdecoxib on silica gel with toluene - ethyl acetate 1:1. Quantitative evaluation by densitometry at 262 nm. Valdecoxib was well separated from rofecoxib. Linearity was between 800 and 1000 ng/zone. Recovery was 98.9 %.

Br. by High-Performance Thin-Layer Chromatography. J. Planar Chromatogr. 20, 117-120 (2007). HPTLC of ursolic acid (3beta-hydroxyuro-12-enoic acid) on silica gel prewashed with methanol in a twin-trough chamber with toluene - ethyl acetate - triethylamine - methanol 7:2:1:1. After derivatization with Liebermann-Burchard reagent the chromatograms were evaluated densitometrically at 366 nm in the fluorescence mode.

J. Sep. Sci. 30, 2167-2172 (2007). HPTLC of clozapine in human serum on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 290 nm. Linearity was between 10 and 100 ng/zone. The intra-assay variation was between 2.10 and 3.33 % (n=5) and inter-assay variation was between 2.67 and 4.44 % (n=9). The limits of detection and quantification were 0.03 and 0.05 ng/µL, respectively. Recovery was between 97.0 and 99.0 %, and selectivity regarding matrix was given.

Indian J. Pharm. Sci. 69(4), 594 (2007). HPTLC of nebivolol HCl in tablet dosage form, on silica gel with ethyl acetate - toluene - methanol - ammonia 10:60:20:1. Quantitative evaluation by densitometry at 280 nm. The hRf value was 33. Linearity was in the range of 100 - 600 ng/mL. Limit of detection and quantification was 30 and 100 ng/zone, respectively. The method was compared with an RP-HPLC method in respect of different analytical parameters. The RP-HPLC and the HPTLC method were found comparable, but the HPTLC method was more sensitive.

J. Planar Chromatogr. 20, 451-456 (2007). HPTLC of levetiracetam and oxcarbazepine on silica gel, prewashed with methanol, with toluene - acetone - methanol 3:1:1 in a twin-trough chamber saturated for 20 min at 25 °C. Densitometric evaluation in absorbance mode at 200 and 261 nm.