Marine Drugs 19(3), 161 (2021). Samples were a standard mix (tripalmitin, palmitic acid, cholesterol, phosphatidylcholine) and lipid-enriched extracts of zebrafish larvae (Danio rerio, Cyprinidae), that were anesthetized with tricaine after having being treated with 11 extracts of cyanobacteria strains and/or with a green fluorescent lipid analogue of fatty acids (BODIPY-C16, bore-dipyrromethene derivative). HPTLC on nano silica gel in 3 steps: 1) and 2) with chloroform – methanol – water 12:6:1 (twice up to 4 cm); 3) hexane – diethyl ether – acetic acid 160:40:3 (once up to 9 cm). Derivatization of lipids by spraying primuline solution (0.01 % in acetone – water, 3:2). Quantification based on fluorescence peak area intensity, was performed using image software on pictures taken through a green fluorescence imager. Triglycerides were decreased in the case of larvae treated with 2 extracts of Synechocystis strains (Merismopediaceae), but the levels of other lipid classes were not affected. No treatment significantly affected the incorporation of BODIPY-C16 into any of the lipid classes of the larvae.

Food Control. 136, 108840 (2022). HPTLC of flavonoids, coumarins, sesquiterpene lactones and phenolic acids in German Chamomile (Matricaria recutita L.) on silica gel with ethyl acetate - methanol - water - acetic acid 200:25:20:1 and ethyl acetate - toluene 2:1. Detection of flavonoids and phenolic acids by spraying with Natural product reagent. Detection of sesquiterpene lactones by spraying with anisaldehyde sulfuric acid reagent. Qualitative analysis under UV light at 366 nm. Principal component analysis (PCA) was used for reducing data dimensionality and representing samples across principal components.

Food Chem. 408, 135253 (2023). HPTLC of genotoxic compound zones in 33 oils on silica gel with chloroform - ethanol 5:1, up to 20 mm, then with n-hexane - diethyl ether 2:1, up to 40 mm, and finally with n-hexane - toluene 1:2 up to 60 mm. Detection in white light, UV 254 nm and 366 nm. Genotoxicity bioassay by spraying with Salmonella suspension with or without the S9 mixture (fraction from phenobarbital/β-naphthoflavone-induced lyophilized rat liver strain), followed by incubation at 37 °C for 3 h. The plate was dried and FDG (fluorescein di(β-D-galactopyranoside)) was sprayed, followed by incubation at 37 °C for 15 min. Cytotoxicity bioassay by spraying with MTT solution (0.2 % in phosphate buffer) after incubation with the Salmonella culture, followed by analysis under white light. Confirmative detection of aliphates was performed via a reagent sequence on the same plate by spraying with 1) Rhodamine 6B reagent, followed by detection in UV 366 nm and 2) phosphomolybdic acid reagent, followed by heating at 120 °C for 10 min, and documented at white light illumination.

Food Chem. 383, 132597 (2022). HPTLC of protodioscin and escin in Camellia and Fenugreek on RP-18 with methanol - water - formic acid 65:35:1. Detection by spraying with 0.5 % p-anisaldehyde in a sulfuric methanol solution, followed by heating at 150 °C until visualization of zones.

Food Chem. 133784 (2022). Review of enzymatic protein hydrolysis, including standard and conventional techniques and their applications and insights into new strategies of detection and characterization of bioactive peptides. The paper described HPTLC methods coupled with bioassays in effect-directed analysis (EDA) to detect the bioactivities of peptides.

Food Chem. 133992 (2023). HPTLC of kiwi peel extracts on silica gel with ethyl acetate - formic acid - acetic acid - water 100:11:11:26. Detection of phenolics and tanning agents by heating the plate at 100 °C for 2 min, followed by dipping into fast blue B (140 mg fast blue salt B in a mixture of 140 mL methanol, 10 mL water, and 50 mL dichloromethane). Detection of flavanols, phenols and further natural compounds by dipping into a NPA solution (1 g 2-aminoethyl diphenylborinate in 200 mL of ethyl acetate), followed by drying, detection under UV light at 366 nm, followed by dipping into the anisaldehyde reagent (0.5 mL anisaldehyde, 10 mL acetic acid, 85 mL methanol, and 10 mL sulphuric acid), followed by heating at 100 °C for 5 min. Antioxidants were detected by dipping into a DPPH solution (0.4 g 2,2-diphenyl-1-picrylhydrazyl in 200 mL of methanol), followed by incubation in dark for 30 min. 

Food Chem. 132937 (2022). Review of TLC assays, including cellular, enzymatic and chemical, and their application for effect-directed food-related analysis, in the last five years. TLC assays for the analysis of antioxidants and for the detection of enzyme inhibitors were described. Microorganism based TLC assays for the detection of antimicrobials, aflatoxins and estrogenic compounds were also presented. TLC methods for the detection of bisphenol and dioxin-like chemicals were described. 

Food Chem. 131846 (2022). HPTLC of Trifolium medium L. (1) and Trifolium pratense (2) on silica gel with dichloromethane - methanol - ethyl acetate 5:10:11:20 for (1) and chloroform - ethyl acetate 3:2 for (2). The AChE inhibitory activity of individual compounds was examined by spraying with 1) acetyl cholinesterase, followed by incubation at 37 °C for 20 min, 2) 2 mM acetylthiocholine solution and 3) 2 mM 5,5´-dithiobis-(2-nitrobenzoic acid) solution, and visualized after 20 min.