J. Chromatogr. A 1155 (1), 112-123 (2007). Presentation of a novel strategy for the fractionation of complex peptide mixtures using two-dimensional planar electrochromatography/thin-layer chromatography (2D PEC/TLC). It was found that phosphopeptides migrate more slowly in the first dimension, based upon their anionic phosphate residues, and certain predominantly acidic phosphopeptides even migrate in the opposite direction, relative to the bulk of the peptides. Further distinguishing phosphopeptides based upon hydrophilicity in the second dimension, which permits a restricted region of the plate to be directly interrogated for the presence of phosphopeptides by MS. Discussion of peptide sequencing and identification of phosphopeptide from the plates by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)-MS and tandem MS.

J. Planar Chromatogr. 20, 131-133 (2007). HPTLC of camptothecin (4-ethyl-4-hydroxy-1H-pyrano[3’,4’:6,7]indolizino[1,2b]quinoline-3,14(4H,12H)dione) on silica gel with toluene - acetonitrile - glacial acetic acid 65:35:1. Detection and quantitation were performed by densitometric scanning in fluorescence mode at 370 nm.

59th Indian Pharmaceutical congress C-299, 296, (2007). The gastroprotective drug Gastse was prepared by mixing standardized alcoholic extracts of Capsicum annum, Chelidonium majus, Strychnos nux vomica, and Arsenicum album. HPTLC of Gastse on silica gel with n-hexane - ethyl acatate 52:6. Evaluation at UV 254 nm and 366 nm. Densitometric determination at 200 nm and 400 nm.

Chromatographia 67 (1-2), 101-107 (2008). HPTLC of simvastatin and ezetimibe on silica gel with n-hexane – acetone 3:2. Quantification by densitometry in absorbance mode at 234 nm. The hRf value of simvastatin was 39 and of ezetimibe 50. Linearity was between 200 and 1600 ng/spot with correlation coefficients r2 = 0.9917 for simvastatin and r2 = 0.9927 for ezetimibe. Limits of detection and quantitation were 25 and 150 ng per band, respectively. For investigation of stability simvastatin and ezetimibe were subjected to acid, pH 6.8 phosphate buffer, oxidation, dry heat, and wet heat. The degradation products were well resolved from the pure drug, therefore the method could be effectively used for stability-indicating analysis.

59th Indian Pharmaceutical Congress C-220, 277, (2007). HPTLC of gallic acid in fruits of Emblica officinalis, on silica gel with touene - ethyl acetate - acetic acid - formic acid 4:9:4:3. Densitometry at 277 nm for quantitative evaluation. The method was linear in the range of 10 and 1000 ng/mL, with an average recovery of 101.4 %. The method was found suitable for routine quality check-up of Emblica officinalis.

59th Indian Pharmaceutical Congress C-66, 240, (2007). Evaluation of Arjunakhseerpak and Arjunaristha, two ayurvedic formulations used for cardiovascular system, which contain Terminalia arjuna as the main ingredient. Simultaneous HPTLC of arjungenin and arjunolic acid from Terminalia arjuna on silica gel with toluene - ethyl acetate - acetic acid 10:10:1. Detection by spraying with 10 % sulphuric acid followed by heating. Densitometric evaluation at 366 nm. The UV spectra of the sandards arjungenin and arjunolic acid was found to be similar to that of arjungenin and arjunolic acid in the formulation. The method was found suitable for standardization of arjuna formulations.

J. Planar Chromatogr. 20, 139-140 (2007). HPTLC of eugenol (4-allyl-2-methoxyphenol) on silica gel with heptane - ethyl acetate 3:2 in normal, unsaturated chambers. Visualization under UV light at 254 nm.

J. Planar Chromatogr. 20, 73-74 (2007). HPTLC of hedychenone (a trimethyldecalin terpene) on silica gel with n-hexane - ethyl acetate 4:1 in a saturated twin-trough chamber. After development the plates were dried at 105 °C for 20 min. Densitometric evaluation in absorbance mode at 254 nm.