Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      110 077
      Rapid isolation and HPTLC validated method for the quantification of echioidinin-5-O-beta-D-glucopyranoside in Andrographis echioides
      B. GHULE*, P. YEOLE (*Institute of Pharmaceutical Education and Research, Borgaon (Meghe), Wardha-442001, Maharashtra State, India, ghulebv@rediffmail.com)

      J. Planar Chromatogr. 25, 575-580 (2012). HPTLC of echioidinin-5-O-beta-D-glucopyranoside in Andrographis echioides on silica gel with chloroform - methanol 31:9. Quantitative determination by absorbance measurement at 254 nm. Linearity was in the range of 200-1400 ng/zone. Limits of detection and quantification were found to be 54 and 68 ng/zone, respectively. Recovery (by standard addition) was 96.7 %.

      Classification: 32e
      110 115
      Hyphenated high-performance thin-layer chromatography for profiling of some indian natural efficiency enhancers
      KShipra MISRA, R. TULSAWANI, R. SHYAM, D. MEENA, Gertrud MORLOCK* (*Institute of Food Chemistry, University of Hohenheim, Garbenstrasse 28, 70599, Stuttgart, and Justus-Liebig-University of Giessen, Institute of Nutritional Science, IFZ, Heinrich-Buff-Ring 26, 35392 Gieben, Germany, gerda.morlock@uni-hohenheim.de)

      J. Liq. Chromatogr. Relat. Technol. 35, 1429-1443 (2012). Comprehensive HPTLC profiling of sugars, sugar alcohols, amino acids, flavonoids, and phenolic acids as well as further bioactive and antioxidative compounds in the Indian natural efficiency enhancer herbs Hippophae rhamnoides, Valeriana wallichii, Triticum aestivum, and fungus Cordyceps sinensis. HPTLC on silica gel with different mobile phases: for sugars, n-butanol–- isopropanol – acetic acid – boric acid solution 6:14:1:3, for amino acids, n-butanol – ammonia (25%) – pyridine – water 39:10:34:26, and for sugar alcohols, ethanol – ethyl acetate – water 7:2:1. Quantitative determination of sugars by absorbance measurement at 370 nm after derivatization with aniline diphenylamine o-phosphoric acid reagent (mixture of 70 mL aniline solution and 70 mL diphenylamine solution, 2 % in acetone each, and 10 mL o-phosphoric acid, 85%). Unknown marker compounds were characterized by HPTLC-attenuated total reflection fourier transform infrared spectroscopy (HPTLC-ATR FTIR) and HPTLC- electrospray ionization mass spectrometry (HPTLC-ESI-MS).

      Classification: 32e
      110 138
      Comparison of TLC and HPLC fingerprints of phenolic acids and flavonoids fractions derived from selected Sage (Salvia) species
      M. SAJEWICZ, D. STASZEK, M. HAJNOS, Teresa KOWALSKA* (*Institute of Chemistry, University of Silesia, 9 Szkolna Street, 40-006, Katowice, Poland, teresa.kowalska@vs.edu.pl)

      J. Liq. Chromatogr. Relat. Technol. 35, 1388-1403 (2012). HPTLC fingerprint of phenolic acids and flavonoids in 23 different sage species on silica gel with different mobile phases. When compared with spectrophotometric and HPLC/DAD methods, the HPTLC approach was a sufficient alternative by quickly providing a valuable complementary fingerprint material. HPTLC of 1) free phenolic acids and phenolic acids fractions derived from acid and basic hydrolysis, with benzene – ethyl acetate – formic acid 6:3:1; 2) Flavonoid aglycons with toluene – ethyl acetate –- formic acid 12:6:1; 3) basic flavonoid glycosides and acidic flavonoid glycosides fractions with ethyl acetate – water – formic acid – acetic acid 100:26:11:11. Detection under UV light at 366 nm.

      Classification: 32e
      111 005
      Localization in adsorption chromatography
      L. SNYDER (LC Resources, 26 Silverwood Ct., Orinda, CA 94563, USA, snyder0036@comcast.net)

      J. Planar Chromatogr. 25, 184-189 (2012). The author reviewed the effects of the localization of molecules of solute or solvent on the adsorbent surface in adsorption chromatography, particularly on sample retention and resolution.

      Classification: 2c
      111 025
      Lipophilicity of oils and fats estimated by TLC
      R. NASCU, C. SARBU* (*Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, Arany Janos Str. No. 11, Cluj Napoca, 400028, Romania, csarbu@chem.ubbcluj.ro)

      J. Sep. Sci. 36, 1317-1326 (2013). HPTLC of 1. aflatoxine G1; 2. ochratoxine A; 3. zearalenone; 4. quinine; 5. cinconine, 6. hydroquinine; 7. o-(4-chlorobenzoyl) hydroquinine; 8. hydroquinine 4-methyl-2-quinolyl ether; 9. (DHQ)2 Phal; 10. (DHQ)2 AQN and 11. camptothecin on RP-18, RP-8, and RP-2 with 85 to 95 % methanol in steps of 2.5 %. In the case of the other stationary phases, the methanol fraction modification was of 5 %. The methanol fraction ranged from 75 to 95 % for RP-18 W, from 35 to 55 % for amino phase, from 50 to 70 % for diol phase, and finally from 65 to 85 % for cyano phase. Qualitative identification by absorbance measurement at 254 and 366 nm. The lipophilicity indices indicated that the mRM appears to be the most suited descriptor.

      Classification: 11c
      111 044
      Chromatographic behavior and separation of pesticides on thin silica gel layers impregnated with cationic micelles
      A. MOHAMMAD*, A. AMIN, A. MOHEMAN (*Department of Applied Chemistry, Aligarh Muslim University, Aligarh, India, alimohammad08@gmail.com)

      J. Planar Chromatogr. 25, 101-107 (2012). HPTLC of mixtures of the pesticides glyphosate, acephate, chlorpyrifos, malathion/methyl parathion, and isoproturon on silica gel impregnated with 0.01 % CTAB (Ncetyl-N,N,N-trimethyl ammonium bromide) and developed with hexane - acetone 1:1. LOD of the pesticides was aproximately 20 µg/band. The method can also be applied for fast determination of pesticides in cereals, vegetables and fruit grains.

      Classification: 29f
      111 068
      Development and validation of HPTLC method for simultaneous estimation of propranolol hydrochloride and flunarizine dihydrochloride in combined tablet dosage form
      A. DOSHI*, B. PATEL, C. PATEL (*Department of Quality Assurance, Shri Sarvajanik Pharmacy College, Mehsana, Gujarat, India, doshi007.1989@gmail.com)

      J. Planar Chromatogr. 26, 62-66 (2013). HPTLC of propranolol hydrochloride (1) and flunarizine dihydrochloride (2) on silica gel with methanol - chloroform - toluene 6:14:14+1 drop glacial acetic acid. Quantitative determination by absorbance measurement at 260 nm. The hRf values of (1) and (2) were 35 and 64, respectively. Linearity was in the range of 3000-15000 ng/zone and 750-3700 ng/zone for (1) and (2), respectively. Intermediate precision was below 2 %.

      Classification: 32a
      111 106
      Quantitative estimation of lupeol and stigmasterol in Costus igneus by high-performance thin-layer chromatography
      K. MANJULA, K. RAJENDRAN*, T. EEVERA, S. KUMARAN (*Department of Biotechnology, Periyar Maniammai University, Tamil Nadu-613 403, India, nkraj64@yahoo.co.uk)

      J. Liq. Chromatogr. Relat. Technol. 36, 197-212 (2013). HPTLC of lupeol (1) and stigmasterol (2) in the stems of Costus igneus on silica gel with n-hexane - ethyl acetate 4:1 for (1) and toluene - acetone - acetic acid 89:9:2 for (2). Detection by dipping in a solution of 0.5 mL p-anisaldehyde in 50 mL glacial acetic acid and 1 mL of 97 % sulfuric acid, followed by heating at 105 °C. Quantitative determination by absorbance measurement at 538 nm. The hRf values of (1) and (2) were 55 and 58, respectively. Linearity was in the range of 5-10 µg/zone for (1) and 1-6 µg/zone for (2). LOD and LOQ were 131 and 430 ng/zone for (1) and 80 and 212 ng/zone for (2). Intermediate precision was below 2.9 %. Average recovery (by standard addition) for (1) and (2) was 100.2 % and 99.9 %, respectively.

      Classification: 32e
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