J. Liq. Chromatogr. Relat. Technol. 41, 324-328 (2018). TLC minilab protocols were transferred to HPTLC validated methods for the determination of atenolol (1), chloramphenicol (2), furosemide (3), glibenclamide (4), penicillin V potassium (5), and praziquantel (6) on silica gel with methanol – ammonia 100:1 for (1), ethyl acetate – toluene – methanol 15:5:3 for (2), toluene – ethyl acetate – acetic acid 17:13:1 for (3), ethyl acetate – methanol – toluene – ammonia 11:7:1:1 for (4), ethyl acetate – methanol – acetic acid 20:10:1 for (5) and acetone – toluene 14:7 for (6). Qualitative evaluation under UV 254 nm. The hRf values for (1) to (6) were 35, 52, 30, 61, 48 and 65, respectively.

J. Planar Chromatogr. 31, 497-504 (2018). HPTLC of chloroacetamide derivatives on RP-18 with ethanol and tetrahydrofuran as organic modifiers with water. Lipophilicity was examined by cluster analysis and principal component analysis. The obtained chromatographic parameters of the examined acetamides were correlated with the standard measure of lipophilicity, log P.

germplasms from Western Himalayas. Pharmacogn. Mag. 14, 141-146 (2018). HPTLC of precocene I (1) and precocene II (2) in the germoplasms of Ageratum conyzoides on silica gel with toluene – ethyl acetate 49:1. Quantitative determination by absorbance measurement at 300 nm. The hRF values (1) and (2) were 60 and 23, respectively. Linearity was between 100 and 500 ng/zone for both (1) and (2). LOD and LOQ were 5 and 16 μg/mL for both (1) and (2). The intermediate precision was <5 % (n=3). Average recovery was 99.7 % for (1) and 102.9 % for (2).

Pharmacogn. Mag. 14, 45-51 (2018). HPTLC of stigmasterol in Monochoria vaginalis and Monochoria hastata on silica gel with chloroform – methanol 4:1. Detection by spraying with anisaldehyde-sulfuric acid reagent, followed by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 540 nm. The hRF value for stigmasterol was 30. Linearity was between 1000 and 5000 ng/zone. LOD and LOQ were 80 and 200 ng/zone. The intermediate precision was <3 % (n=3). Average recovery was 99.8 %.

J. Agric. Food. Chem. 66, 13378-13385 (2018). HPTLC of 2,4-dichlorophenoxyacetic acid (2,4-D) metabolites in the leaves of wild radish (Raphanus raphanistrum L., Brassicaceae) on silica gel with toluene – 2-butanone – acetic acid 45:55:3. Qualitative identification using [14C]-2,4-D-treated plants. The hRF values for 2,4-D and metabolite 3 were 50 and 32, respectively.

J. AOAC Int. 101, 1031-1041 (2018). HPTLC of zofenopril calcium (1) and hydrochlorothiazide (2) in the presence of the hydrochlorothiazide impurities chlorothiazide (3) and salamide (4) on silica gel with ethyl acetate – glacial_x000D_ acetic acid – triethylamine 100:1:1. Quantitative determination by absorbance measurement at 270 nm. The hRF values for (1) to (4) were 34, 44, 25 and 64, respectively. Linearity ranged between 0.5-10.0 μg/zone for (1), 0.2-4.0 μg/zone for (2), 0.05-1.4 μg/zone for (3) and 0.05-1.0 μg/zone for (4). LOD and LOQ were 20 and 50 ng/zone for (3) and (4). The intermediate precision was <2 % (n=3). Average recovery was 100.0 % for (1), 99.6 % for (2), 99.5 % for (3) and 99.2 % for (4).

CBS 85, 9 (2000). HPTLC of pot fragment extracts on RP-18 plates developed twice at 4 °C with first methanol - acetonitrile - THF 8:2:1 over 20 mm and second methanol - acetonitrile - THF 18:2:1 over 80 mm after pre-chromatographic derivatization with 1 % N,N’-dicyclohexylcarbodiimide in dichloromethane followed by dansyl semicadaverine in dichloromethane. After drying the plate was dipped in 4 % Triton X100 in hexane. Evaluation at 366/>400 nm.

Analysis of choline esterase inhibitors. CBS 83, 4-5 (1999) HPTLC-AMD of choline esterase inhibitors on silica gel with a 15-step gradient from methanol via dichloromethane to hexane. Detection by spraying with butyryl choline esterase solution followed by incubation at 37 °C for 30 min, and spraying with fast blue salt mixed with alpha-naphthyl acetate followed by incubation at 37 °C for 5 min. Quantitative determination by absorbance measurement at 254 nm.