Ind. J. Pharma. Sci. 71(4), 451-454 (2009). HPTLC of clotrimazole in bulk drug and tablet dosage form on silica gel with cyclo hexane - toluene - methanol - triethyl amine 80:20:5:2. Quantitative determination by absorbance measurement at 262 nm. The calibration curve was linear between 200 to 1000 ng/spot for clotrimazole. The limit of detection and limit of quantification for clotrimazole were 50 ng/spot and 200 ng/spot, respectively.

Abstract No. 9185, IHCB (2009). HPTLC of hydro alcoholic extracts of Berberis aristata on silica gel with benzene - ethyl acetate - diethyl amine 6:3:1. Detection by spraying with AlCl3 reagent (for estimation of flavonoids) or with Folin Ciocalteu reagent (for total phenolic content). The fingerprint profile was optimized using two different mobile phases: n-butanol - acetic acid - water 14:1:5 and n-propanol - formic acid - water 90:1:9. The extract showed 6 different spots and was found to contain 13.5 % w/w of berberin.

Abstract No. F-275, 61st IPC (2009). HPTLC of ivabradine HCl on silica gel with methanol - chloroform 1:1. The hRf value was 59. Quantitative determination by absorbance measurement at 285 nm. Linearity was in the range of 100-800 ng/spot with r2=0.9989 (via peak area).

J. Planar Chromatogr. 22, 273-276 (2009). HPTLC of bicalutamide and leflunomide (as internal standard) on silica gel with toluene - ethyl acetate 4:5 in a twin trough chamber saturated for 30 min at room temperature. Quantitative determination by absorbance measurement at 273 nm. The limit of detection and quantification was 50 and 200 ng/band, respectively.

J. Chromatogr. A 1217 (23), 3702-3706 (2010). Evaluation of the chromatographic behavior of some artificial and natural sweeteners by HPTLC on RP18, RP18W, RP8, cyano and amino phases with mixtures of acetonitrile - water in different volume proportions. The lipophilicity is given through chromatographic descriptors such as RM0, mean of RM (mRM), and scores of RM values corresponding to the first principal component (PC1/RM). In addition, scores and loadings resulting from covariance matrix of retention data provide new information about similarity and differences of investigated compounds and between the stationary phase and the mobile phases. The experimental lipophilicity indices estimated from retention data were correlated with computed values, via computer software and internet module. Results were in accordance at a highly significant statistical level.

International Seminar on Herbal Drug Research, PN-028 (2009). HPTLC of curcumin and 3-acetyl-11-keto-a-boswellic acid in the herbal product Rheumax (contains Curcuma longa, Boswellia serrata, Tinospora cordifolia and Vitex negundo) on silica gel with chloroform - methanol 37:3 for curcumin and n-hexane - ethyl acetate 1:1 for the acid. Quantitative determination by absorbance measurement at 430 nm for curcumin and 254 nm for the acid. The method was linear in the range of 100-500 ng/band for curcumin and 1500-4000 ng/band for the acid.

Abstract No. C-372, 61st IPC (2009). HPTLC of withanolide A in extracts and spansules dosage form on silica gel with toluene - ethyl acetate - formic acid 8:6:1. Densitometric evaluation at 254 nm. Withanolide A was well separated with an hRf value of 14. The linearity range was 40-200 ng/band.

J. Planar Chromatogr. 23, 208-211 (2010). HPTLC of prednisolone (and hydrocortisone as impurity) on silica gel (prewashed with methanol) with chloroform - methanol 19:1 in a twin-trough chamber previously saturated for 30 min. Quantitative determination by scanning densitometry at 250 nm. Linearity was in the range of 2-10 µg/band (r = 0.9967, calculated via peak area). LOD and LOQ was 200 and 600 ng/spot, respectively. Recovery was 100.0 % for prednisolone. Repeatability was 0.74 % and the inter-day (n = 6) and intra-day (n = 12) precision was 2.6 and 2. 7 %, respectively.