J. Pharm. Biomed. Anal. 37, 817-821 (2005). CO2 extracts of Harpagophytum procumbens root was evaluated by HPLC and HPTLC for harpagoside contents. HPTLC on silica gel with ethyl acetate - methanol - water 77:15:8 in saturated ADC chamber. Detection by dipping into anisaldehyde reagent followed by drying at 120 °C for 5 min. Quantitative determination by absorbance measurement at 509 nm. The linearity range was 0.04-0.40 mg/mL. The HPTLC method was less time consuming than HPLC, needing almost no sample pre-treatment. 15 different CO2-extracts of the plant were analysed.

J. Planar Chromatogr. 18, 282-284 (2005). TLC and HPTLC of horseradish and mustard samples on silica gel with iso-propanol - 25 % ammonia 9:1 containing different volumes of water (1, 2, 3, or 5 parts). After development the compounds were visualized under UV light at 254 nm or by exposure to iodine vapor.

J. Pharm. Biomed. Anal. 39, 801-804 (2005). HPTLC on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 265 nm. The hRf values of stavudine (SV), lamivudine (LV) and nevirapine (NV) were 25, 67 and 87 respectively. The method was linear within the range of 0.01-0.06 mg/mL, 0.05-0.30 mg/mL and 0.06-0.40 mg/mL for SV, LV, and NV respectively, recovery rates were between 98.2 and 99.9 %. The HPTLC method was compared with the UV and HPLC methods. Accuracy, precision and ruggedness of the method were established.

Indian J. Pharm Sciences 67 (6), 687-690 (2005). HPTLC of sparfloxacin extracted with dichloromethane from plasma. A standard solution was prepared in methanol - dichloromethane 1:1. HPTLC on silica gel with chloroform - toluene - methanol - diethylamine 44:15:2:1. Quantitative determination by absorbance measurement at 301 nm. The method had a linearity range of 80-200 ng/spot with an average recovery of 89.17 %.

J. Planar Chromatogr. 19, 228-232 (2006). HPTLC of pantoprazole sodium sesquihydrate (5-difluoromethoxy-2-[(3,4-dimethoxy-2-pyridinyl)methyl]sulfinyl-1H-benzimidazole) on silica gel after prewashing with methanol in a twin-trough chamber presaturated for 10 min with methanol - water - ammonium acetate 8:2:1. Quantitation by densitometric scanning at 290 nm. The method was validated for linearity, sensitivity, precision, accuracy, specificity, system suitability, ruggedness and robustness, and repeatability.

Indian J. Pharm Sciences 67 (6), 762-764 (2005). HPTLC of fenofibrate in methanolic capsule extracts on silica gel with toluene - chloroform 7:3 with chamber saturation for 10 min. Quantitative determination by absorbance measurement at 296 nm. Linearity range was 1.2-3.8 g with recovery of 101.43 %. The proposed validated method was stability indicating and useful for routine analysis.

J. Planar Chromatogr. 19, 238-240 (2006). HPTLC of plant extracts, using pellitorine and dihydropiperlongumine as markers, on silica gel in a twin-trough chamber saturated with the mobile phase hexane - ethyl acetate 3:1. Quantitation by densitometry in absorbance/reflectance mode at 260 nm.

Acta Chrom. 6, 7-12 (1996). HPTLC of fructose, glucose and sucrose on silica gel with concentration zone. Impregnation of the layer (except concentration zone) by spraying with 0.1 M sodium bisulfate solution, followed by drying and spraying with citrate buffer of pH 4.8. Three-fold development with acetonitrile - deionized water 17:3 after chamber saturation. Visualization by spraying with 1-naphthol - sulfuric acid reagent and heating at 110 °C. Quantification by densitometry at 515 nm.