60th Indian Pharmaceutical Congress PA-227 (2008). HPTLC of oxcarbazepine (in acetonitrile extracts of human plasma) on silica gel with ethyl acetate - toluene - methanol 7:2:1. The hRf values of oxcarbazepine and the internal standard chlorzoxazone were 54 and 86, respectively. The linearity range was 10-300 ng/mL, recovery from plasma was 75.2 %.

Abstract No. 9933, IHCB (2009). HPTLC of colchicines in Gloriosa superba (collected from different parts of India) on silica gel with ethyl acetate - methanol 200:27. The hRf value of colchicine was 29. Quantitative determination by absorbance measurement at 350 nm. The method was linear in the range of 50-1000 ng/spot. The sample collected from Kerala was found to contain highest level of colchicines (0.24 %).

Chromatographia 70 (7-8), 1277-1282 (2009). HPTLC of preservatives on three stationary phases of different polarity: RP-18, RP-18W and cyano phase, with methanol – water mixtures in different volume proportions. The resulting RM values showed a linear decrease with increasing methanol concentration of the mobile phase (determination coefficients for all stationary phases were >0.98). The retention behavior of the preservatives on RP phase is in good agreement with their polarity. Principal component analysis showed that for all three stationary phases the same lipophilic interactions take place.

CBS 104, 13-15 (2010). HPTLC of aloeverose, glucose and galactose in aloe vera products on silica gel (impregnated with NaH2PO4) with acetone - iso-propanol - 0.1 M formic acid 2:2:1 (double development for products with high glucose content). Detection by immersion in 4-aminobenzoic acid reagent (1 g 4-aminobenzoic acid in 36 mL acetic acid, with 40 mL water, 2 mL 85 % phosphoric acid and 120 mL acetone), followed by heating at 100 °C for 10 min. Densitometric evaluation by fluorescence measurement at 366 nm. The limit of quantification was approx. 3 % in aloe vera products. RSD for polynomial calibration is 2.1 % for mannose in the range of 8-80 ng/band and 4.0 % using matrix calibration.

J. Planar Chromatogr. 23, 23-27 (2010). HPTLC of ziprasidone (5-[2-[4-(1,2-benzothiazol-3-yl)piperazin-1-yl]ethyl]-6-chloro-1,3-dihydroindol-2-one) on silica gel with hexane - dioxane - propylamine 5:45:2 up to 9 cm (under saturated conditions) and on RP8 with tetrahydrofuran - phosphate buffer (pH 9.0) 1:1 up to 4.5 cm (under unsaturated conditions), both in horizontal chambers. Quantitative determination by videodensitometry at 254 nm. Calibration was linear in the range 0.2-1.2 and 0.1-1.1 µg/spot ziprasidone for NP-HPTLC and RP-HPTLC, respectively. The intra-day precisions for 0.4-1.2 µg/spot on NP-HPTLC was 2.0 to 5.2 % and on RP-HPTLC 4.0 to 6.1 %; the respective inter-day precision for NP-HPTLC was 2.0 to 6.7 % and for RP-HPTLC 4.1 to 7.1 %. LOD/LOQ on NP-HPTLC was 0.03/0.09 µg/spot; using RP-HPTLC, LOD/LOQ was 0.02/0.06 µg/spot. The specificity of the methods was confirmed by comparison of hRf values (74 +/- 2 in NP-HPTLC and 36 +/- 1 in RP-HPTLC, n=12).

J. Ethnopharmacol. 130, 267-271 (2010). HPTLC of evodione and leptonol from the leaves and influorescences of Melicope lunu-ankenda on silica gel with chloroform - methanol 1:1. Detection by spraying with anisaldehyde - sulfuric acid reagent, followed by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 580 nm.

Abstract No. C-37, 61st IPC (2009). HPTLC of methanolic leaf extracts of Aloe vera (after purification with petroleum ether (60-80 °C)) on silica gel with toluene - ethyl acetate - glacial acetic acid - methanol 4:18:1:4. Under UV 254 nm six bands with hRf values of 12, 26, 34, 44, 62, and 84 were observed. These bands correspond to well known constituents of Aloe vera: aloeresin hRf 25, barbaloin hRf 33, aloe emodin hRf 43, emodin hRf 63. The bands with hRf values of 12 and 84 could not be identified. The reported finger print profiling can serve as potential technique for authentification and batch to batch consistency of herbal drugs.

Talanta, 80 (3), 1045-1051 (2010). Review on chromatographic methods for synthetic food dyes, including the following techniques: TLC, HPTLC, traditional column chromatography, HPLC, ion-pair chromatography, RP HPLC, and high performance ion chromatography, demonstrated by using examples in different conditions for each technique.