J. Ethnopharmacol. 235, 361-374 (2019). HPTLC of colchicine (1) and withaferin A (2) in the polyherbal ayurvedic formulation Peedantak Vati (PV) on silica gel with chloroform - 
methanol - water - formic acid 140:13:2:4. Quantitative determination by absorbance measurement at 218 nm. The hRF values for (1) and (2) were 22 and 28, respectively. LOD and LOQ were 40 and 120 ng/zone for (1) and 120 and 240 ng/zone for (2), respectively.  

J. Ethnopharmacol. 236, 474-483 (2019). HPTLC of asiaticoside and madecassoside in Centella asiatica on silica gel with butanol - ethyl acetate - water 4:1:5. Detection by spraying with anisaldehyde-sulfuric acid reagent, followed by heating at 105 ºC for 5 min. Qualitative identification under UV light at 580 nm.    
 

Phytochem. Anal. 30, 405-414 (2019). HPTLC fingerprint of 70 standard medicinal plants on silica gel with ethyl acetate – ethyl methyl ketone – formic acid 98% – water 5:3:1:1. Derivatization with anisaldehyde, Liebermann–Burchard, 3 % iron(III) chloride (FeCl₃) and phosphomolybdic acid reagent. Detection before and after derivatization under visible light and UV light (254 and 366 nm). A similarity search algorithm based on color (RGB, HSV and Lab) information alone or together with hRF values was built  to assess the fingerprinting of medicinal plants. The method showed better results than principal components analysis (PCA), classification and regression trees (CART) and partial least squares discriminant analysis (PLS‐DA).

J. Liq. Chromatogr. Relat. Technol. 30, 311-319 (2019). HPTLC of marker compounds in Porana sinensis on silica gel with ethyl acetate - methanol - formic acid 12:2:1. DPPH* scavenging activity was performed by spraying with 2.54 mM 2,2′‐diphenyl‐1‐picrylhydrazyl methanol solution. Xanthine oxidase inhibitory activity was detected by dipping into a xanthine oxidase staining solution [agar: 0.1 mg/mL; EDTA (ethylenediaminetetraacetic acid): 1 mM; dipotassium hydrogen phosphate/potassium dihydrogen phosphate: 50 mM; NBT (nitro blue tetrazolium): 0.22 mg/mL; xanthine oxidase: 68 mU/mL], followed by incubation for 10 min at 38 °C in an incubation chamber and dipped again in 3 mM solution of xanthine, followed by a second incubation for 20 min at 38° C.

Phytochem. Anal. 30, 679-686(2019). HPTLC-Acetylcholinesterase bioassay of cherimoya fruits on silica gel with the enzyme substrate 1‐naphthyl acetate (1.5 mg/mL). Enzymatic solution was sprayed (1 U/mL in 50 mM Tris–HCl buffers at pH 7.8), followed by incubation at 37 ºC for 10 min. A Fast Blue B salt aqueous solution (1.0 mg/mL), freshly prepared, was sprayed onto the plate to obtain a purple background. Further analysis by HPTLC-MS allowed the characterization of three potential AChE inhibitors: anonaine, glaucine and xylopine.

J. Food Compos. Anal. 80, 16-32 (2019). Review of methods and techniques for the analysis of adulterants in honey, including TLC and HPTLC. Originally TLC was used to detect honey adulterated with high fructose corn syrup. HPTLC has been used for the detection of sucrose, fructose or glucose in commercial honeys.
 

J. AOAC Int. 102, 776-780 (2019). HPTLC of sibutramine, phenolphthalein, and three PDE5 inhibitors (sildenafil, vardenafil and tadalafil), as well as caffeine, fluoxetine, theophylline, and acetaminophen as adulterants in weight loss products on silica gel with methyl tert-butyl ether – toluene – methanol 8:2:1. Densitometric absorption measurement at 225 nm. The hR_F values of reference substances increased in the following order: fluoxetine, sildenafil, vardenafil, caffeine, theophylline, tadalafil, acetaminophen, phenolphthalein and sibutramine. The method was successfully used for the screening of 12 commercial products. Of those, nine products tested positive for at least one undeclared component.

J. Chromatogr. A 1568, 188-196 (2018). Application of an advantageous combination, the desorption-based direct analysis in real time mass spectrometry (DART-MS) immediately after direct bioautography (DB), i.e., in the presence of microorganisms, bioassay medium and substrate reagent. The method offers a straightforward and efficient mass spectrometric detection of bioactive analytes within the bioautogram. It discriminated microorganism cells and highly polar bioassay medium ingredients which could otherwise stress the MS system. Investigation of DB-DART-MS for bioactive compounds in cosmetics using the Bacillus subtilis and Aliivibrio fischeri bioassays for detection of Gram-positive and Gram-negative antimicrobials, respectively, and the planar yeast estrogen screen for detection of estrogen-effective compounds. Study of the influences of three different bioassay matrices on the analyte response and DB-DART-MS performance on different layers (NP and RP) on the example of parabens in hand creams. Ion suppression was enhanced with increasing culture medium complexity. The mass spectrometric quantification by DB-DART-MS at the ng-level in situ each different bioautogram was verified by comparison to HPTLC-DART-MS. The total paraben content of hand creams 1 and 2 was 0.17–0.20% and 0.30–0.34%, respectively, depending on the method used. It proved that DB-DART-MS is a reliable qantitative bioanalytical hyphenation.