CBS 95, 10-13 (2005). HPTLC of five sulfonamides on silica gel with dichloroethane - methanol - 2-propanol - ammonia 25:5:5:1 in the twin trough chamber and ADC 2 with varied chamber saturation. Densitometric evaluation by absorbance measurement at 254 nm. Comparison of chamber saturation in conventional twin trough chamber and Automatic Development Chamber ADC 2 respectively. Reproducibility of Rf-values is better in ADC 2 due to higher quality in chamber saturation and less manual operations.

Lipophilicity of the compounds. J. Planar Chromatogr. 18, 92-97 (2005). TLC of newly synthesized uracil derivatives on silica gel with benzene - methanol, benzene - acetonitrile, benzene - isopropanol, and HPTLC on RP-18 with water - methanol and water - acetonitrile. The mechanism of retention on different TLC supports was investigated and the retention constants determined for the uracils are discussed in terms of the physicochemical properties of both the solutes and stationary and mobile phases. Detection under UV light at 254 nm.

J. Planar Chromatogr. 18, 372-376 (2005). HPTLC of sildenafil in four commercial products and three aphrodisiac herbal preparations on silica gel after pre-saturation with chloroform - methanol - diethylamine 90:10:1. Quantitative determination by absorbance measurement at 305 nm. Recovery was 100.6 and 98.2 % for pure and spiked samples.

J. AOAC Int. 88, 1525-1529 (2005). HPTLC of trandolapril and verapamil in 2-component mixtures and in their combination capsules on silica gel in horizontal chambers with ethyl acetate - ethanol - acetic acid 16:4:1. Quantitative determination by densitometric measurement at 215 nm. Detection and quantitation limits were found to be 1.25 and 3.75 µg/spot for TRA and 0.15 and 0.45 µg/spot for VER, respectively.

J. Chromatogr. B 823 (2), 152-157 (2005). HPTLC of lamotrigine (extracted from serum by ethyl acetate) on silica with toluene - acetone - ammonia 14:6:1. Densitometric measurement at 312 nm, hRf of lamotrigine at 54. The analytical method has excellent linearity (r = 0.998) in the range of 20–300 ng/spot. This assay range is adequate for analyzing human serum, as it corresponds to lamotrigine concentrations measured in human serum from epileptic patients. The method was validated for sensitivity, selectivity, extraction efficiency, accuracy and intra and inter-day reproducibility. The limit of detection and limit of quantification were found to be 6.4 and 10.2 ng, respectively. Good accuracy and high precision (CV) is reported, i.e. in the range of 92.1-97.1 % and 0.5-2.6 % respectively. The method was applied for determination of serum lamotrigine levels in epileptic patients and in pharmacokinetic study of lamotrigine administered orally to rabbits.

Abstract DP-15, IPC (2005). HPTLC of forskolin in herbal extract and opthalmic preparation (prepared from methanolic extracts of Coleus forskohlii roots) on silica gel with toluene - ethyl acetate 17:3. Quantitative determination by absorbance measurement at 292 nm. The method was found to be reproducible, accurate and precise.

J. Planar Chromatogr. 19, 167-170 (2006). HPTLC of standards (cholesterol, oleic acid, triolein, methyl oleate, and cholesteryl oleate as marker compounds for free sterols, free fatty acids, triacylglycerols, methyl esters, and steryl esters, respectively, as well as cholesterol, phosphatidylethanolamine, phosphatidylcholine, and lysophosphatidylcholine for the analysis of polar lipids) and prepared samples of leeches on silica gel (with preadsorbent zone and prescored lanes) in a pre-saturated twin-trough chamber with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 (for neutral lipids) and hexane - petroleum ether - diethyl ether - glacial acetic acid 50:25:5:1 (for steryl esters). Visualization by spraying with 5 % ethanolic phosphomolybdic acid solution and heating for 10 min at 110 °C. HPTLC of polar lipids on equal layer with chloroform - methanol - water 65:25:4. Visualization by spraying with 5% aqueous cupric sulfate solution and heating for 10 min at 140 °C. Quantification of neutral lipids at 610 nm and of polar lipids at 370 nm.

Talanta 60 (5), 945-953 (2003). TLC of linezolid from its alkaline degradation product on silica gel with isobutanol - ammonia 9:1. Quantitative determination by densitometric measurement at 244 nm. The proposed method and two other methods (based on spectrophotometry) were successfully applied to the determination of the drug in bulk powder, in laboratory prepared mixtures with its degradation product and in commercial tablets.