Abstract No. 9154, IHCB (2009). Standardization of Coccinia grandis extract by HPTLC of taraxerol on silica gel with n-hexane - ethyl acetate 9:1 in a twin trough chamber. Detection by spraying with anisaldehyde reagent, followed by heating at 105 °C. Quantitative determination by absorbance measurement at 420 nm. The dichloromethane extract of the plant contained 3.7 % (w/w) of taraxerol.

J. Chinese Trad. Patent Med., 30 (11), 1638-1642 (2008). TLC of Shujin Huoxue capsule extracts on silica gel with 1) petroleum ether (60-90 ºC) - ethyl formate - formic acid 15:5:1; 2) cyclohexane - ethyl acetate 9:1; 3) toluene - acetone - ethanol - ammonia 16:12:1:4. Detection 1) by exposure to iodine vapor; 2) under UV 365 nm; 3) under UV 254 nm. Identification by comparison with the standards of the component drugs. Quantification of paeoniflorin by HPLC.

J. Planar Chromatogr. 22, 363-366 (2009). HPTLC of 5-hydroxymethylfurfural in seven Turkish fruit wines and three Turkish vinegars on silca gel with toluene - ethyl acetate - 90 % formic acid 6:3:1 in a twin trough chamber saturated for 20 min. Quantitative determination by absorbance measurement at 286 nm. The limit of detection and quantification was 0.05 and 0.13 ng/mL, respectively.

J. AOAC Int. 92, 699-702 (2009). HPTLC of a dinitrophenyl-lysine derivative (produced by incubation with 1-fluoro-2,4-dinitrobenzene and hydolyzation with hydrochloric acid) on silica gel (prewashed with methanol) with n-propanol - 25 % ammonia 7:3 in a twin trough chamber. Quantitative determination by absorbance measurement at 360 nm. The method was linear (r2 = 0.9991) in the range from 12.5 to 125.0 ng/band. Repeatability (%RSD) and intermediate precision (%RSD) in matrix were 0.8 % and 3.6 %, respectively. Recoveries of spiked samples at two levels ranged from 72 to 85 % with RSD from 3 to 8%. This method is a reliable, high throughput and low cost analytical method for the salmon feed industry.

Abstract No. F-276, 61st IPC (2009). HPTLC of buclizine hydrochloride on silica gel with methanol - chloroform - ammonia 8:1:1 %. Quantitative determination by absorbance measurement at 234 nm. The calibration curve was linear in the range of 100-700 ng/spot. The limit of detection and limit of quantification were 20 and 100 ng/spot, respectively.

60th Indian Pharmaceutical Congress PA-226, (2008). HPTLC of Neem (active ingredient of Nimbidin capsules) on silica gel with chloroform - ethyl acetate 4:1 containing 1 % of acetic acid. Quantitative determination by absorbance measurement at 254 nm. The chromatogram showed two active constituents in Neem with hRf values of 33 and 55. Linearity was in the range of 2-20 µg. The method was suitable for estimation of different constituents of Neem in herbal formulations.

J. Pharm. Biomed. Anal. 47, 790-794 (2008). HPTLC of the steviolbioside, stevioside and rebaudioside A in Stevia rebaudiana leaves on silica gel with ethyl acetate - ethanol - water 20:5:3. Detection by spraying with acetic anyhdride - sulphuric acid - ethanol 1:1:10 reagent. Quantitative determination by absorbance measurement at 510 nm. Linearity was in the range of 160-960 ng/spot for steviolbioside, 1-6 µg/spot for stevioside and 0.5-3 µg/spot for rebaudioside A with good correlation coefficients (0.998-0.999). The method was used for the assay of steviol glycosides in S. rebaudiana leaves collected from ten different locations.

Abstract No. F-283, 61st (2009). HPTLC of naproxen sodium on silica gel with toluene - ethyl acetate - acetic acid 15:3:2. The hRf value was 63. Quantitative determination by absorbance measurement at 230 nm. The method was linear in the range of 100-500 ng/band.