Phytochem. Analysis 17, 409-413 (2006). HPTLC of gymnemic acid as of gymnemagenin from callus cultures of Gymnema sylvestre on silica gel with chloroform – methanol 4:1. Quantitative determination by absorbance measurement at 205 nm. Linearity of determination of gymnemagenin is between 2 and 10 µg and its average percentage recovery from leave callus extracts is 98.6 - 99.2 %.

J. Chromatogr. A 1127(1-2), 273-277 (2006). HPTLC of sucrose esters from the surface of leaves of Nicotiana tabacum L. on silica gel with n-hexane – ethyl acetate 1:3. Detection by spraying with aniline-diphenylamine reagent. Identification by off-line TLC-MS. Quantitative determination of sucrose esters–insecticides by indirect estimation through TLC of sucrose obtained after alkaline hydrolysis. Application of the method in the screening of sucrose esters in plant extracts in laboratory and field experiments.

J. Liq. Chromatogr. Relat. Technol. 28, 2597-2606 (2005). HPTLC of free sterol, and free fatty acids (cholesterol, triacylglycerol and methyl esters) on silica gel (prewashed with dichloromethane - methanol 1:1) with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 in a twin-trough chamber saturated for 15 min. Determination of steryl esters with n-hexane - petroleum ether - diethyl ether - glacial acetic acid 50:25:5:1. Detection by spraying with 5 % ethanolic phosphomolybdic acid solution and heating for 10 min at 115 °C. Determination of polar lipids (cholesterol, phosphatidyl ethanolamine, phosphatidylcholine, lysophosphatidylcholine) with chloroform - methanol - water 65:25:4. Detection by spraying with a 10 % cupric sulfate solution and heating at 140 °C for 10 min. Quantitation by densitometry at 610 nm (for neutral lipids) and 370 nm (for polar lipids).

Ind. J. Pharm. Sci. 68 (6), 838-840 (2006). HPTLC of ofloxacin and ornidazole in tablet dosage form on silica gel with n-butanol - ethanol - ammonia 5:5:4. Quantitative determination by absorbance measurement at 295 nm. The method was found to be linear in the concentrate range of 1-5 ng/spot with recovery of 99.5-102.5 % for both compounds. The method was validated for linearity, accuracy, precision, repeatability, and specificity.

J. Liq. Chromatogr. Relat. Technol. 28, 677-691 (2005). HPTLC of podophyllotoxin and podophyllin on RP-18 in a twin trough chamber with acetonitrile - water 2:3. Densitometric measurement of lignans in absorption mode at 217 nm.

J. Planar Chromatogr. 20, 251-257 (2007). TLC with aqueous ammonia-organic modifier (acetonitrile, dioxane, acetone) mobile phases has been used to study the effect on retention of the chromatographic system and the physicochemical properties of twelve 2,4-dioxotetrahydro-1,3-thiazoles. Principal-component analysis and partial least-squares regression were used to determine the molecular properties with the greatest effect on retention for each modifier. Good correlation was obtained between experimental and calculated retention data. HPTLC on RP-18 without chamber saturation. Detection under UV 254 nm.

J. Pharm. Biomed Anal. 45(2), 337-340 (2007). HPTLC of oleanolic acid and ursolic acid on silica gel impregnated with 1 % iodine solution in chloroform after sample application. Development with petroleum ether - ethyl acetate – acetone 82:18:1. Detection by spraying with a solution of 10 % sulfuric acid in ethanol followed by heating at 120 °C for 3 min. Quantification by densitometry in absorbance mode at 530 nm.

59th Indian Pharmaceutical Congress F-95, 414, (2007). HPTLC of granisetron hydrochloride on silica gel aluminium plates with chloroform - methanol 4:1, with 0.1 mL of ammonia. The hRf value of granisetron hydrochloride was 42. Densitometry in absorbance mode at 301 nm. Linearity was between 400 and 1600 ng/zone. The limit of detection and quantification was 80 ng and 160 ng/zone, respectively. The drug was subjected to acid and alkali hydrolysis, oxidative and thermal degradation. The degration products were well separated from the main compound.