J. Planar Chromatogr. 27, 346-356 (2014). HPTLC fingerprint of the leaves of Arctium lappa and Fagus sylvatica, herba of Epilobium hirsutum, Lythrum salicaria, and Artemisia dracunculoides; and fruits of Cydonia oblonga and leaves of Hippophae rhamnoides and Aronia melanocarpa on silica gel with ethyl acetate – acetic acid – formic acid – water 50:6:6:13 for flavonoid glycosides and chloroform – acetic acid – methanol – water 16:8:3:2 for saponins. Detection by immersion into natural product reagent followed by PEG 400 reagent. Qualitative identification at UV 366 nm.

J. Liq. Chromatogr. Relat. Technol. 38, 1104-1108 (2015). HPTLC of trans-resveratrol on silica gel with ethyl acetate - cyclohexane - n-butanol 9:9:2. Detection through chemiluminescence by dipping into a TCPO solution (250 mg bis(2,4,6-trichlorophenyl)oxalate in 36 mL n-butyl acetate, followed by adding 0.4 mL hydrogen peroxide that was vigorously shaken with the solution for 20 min). The HPTLC plate was covered by a glass plate and measured for 2 min using a very light-sensitive CCD camera. The hRF value for trans-resveratrol was 78. Linearity was in the range of 20-500 ng/zone. LOD and LOQ were 13.7 and 20.3 ng/zone, respectively.

S. Afr. J. Bot. 100, 122-131 (2015). HPTLC fingerprint of Agathosma betulina and Agathosma crenulata on silica gel with toluene - ethyl acetate - methanol - acetonitrile - formic acid 10:3:3:2:2. Qualitative identification at UV 366 nm. The hRF values of rutin, chlorogenic acid and kaempferol were 15, 25 and 65.

J. Planar Chromatogr. 28, 333-336 (2015). HPTLC of glucose-6-phosphate and glucose-6-phosphate dehydrogenase (G6PDH) on silica gel previously dipped into 100 mM Tris-HCl buffer (pH 7.8) and activated at 110 °C for 40 min, with 100 mM Tris-HCl buffer (pH 7.8) containing coenzyme NAD+ with the desired concentration. Quantitative determination of NADH product by absorbance measurement at UV 340 nm. The method allowed on-plate assay of the G6PDH enzyme activity.

J. AOAC Int. 98, 850-856 (2015). Review of the development of planar chromatography-direct bioautography methodology, with special emphasis on its use as a biomonitoring system. HPTLC of cis-spiroether (1), trans-spiroether (2), alpha-bisabolol (3), bisabolol oxides (4) and the fluorescent coumarin derivatives herniarin (5), and umbelliferone (6) in chamomile extracts on silica gel with chloroform - acetone 99:1. Detection by dipping into vanillin–sulfuric acid reagent (40 mg vanillin, 10 mL ethanol and 200 mL concentrated sulfuric acid) followed by heating at 110 °C for 5 min. Bioassays were performed by the direct bioautographic system using a Gram-negative test bacteria (Xanthomanas euvesicatoria), the luminescence gene-tagged Arabidopsis pathogen (Pseudomonas syringae pv. maculicola) and the luminescent marine bacterium (Aliivibrio fischeri) as well as a Gram-positive soil bacterium (Bacillus subtilis).

J. AOAC Int. 98, 857-861 (2015). _x000D_TLC direct bioautography of apigenin (1) and α-linolenic acid (2) in Matricaria recutita L. (chamomile) and Achillea millefolium L. (yarrow) tinctures on silica gel with chloroform - acetone 99:1. Bioautography by dipping into suspensions of Bacillus subtilis, S. aureus, S. epidermidis, Pseudomonas syringae pv. maculicola, Xanthomonas campestris pv., Escherichia coli and Aliivibrio fischeri for 10 s. In the case of soil bacteria and human pathogens, the plates were incubated at 37 °C for 5 h, followed by dipping into a solution of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 0.05 g/90 mL) for 5 s and further incubation at 37 °C for 2 h. The hRF values for (1) and (2) were 33 and 66, respectively.

J. Ethnopharmacol. 175, 324-334 (2015). HPTLC of betaine in Achyranthes aspera root extract on silica gel with methanol - water 9:1. Detection by spraying with Dragendorff's reagent followed by 10 % ethanolic sulfuric acid and drying at 110 °C for 5 min. Quantitative determination by absorbance measurement at 520 nm. The hRF value for betaine was 36. Linearity was in the range of 1-5 μg/zone. LOD and LOQ were 0.10 and 0.13 μg/zone. The intermediate precision was below 2 % (n=3).

J. Planar Chromatogr. 28, 352-353 (2015). HPTLC of imidacloprid on silica gel with dichloromethane – isopropyl alcohol 1:3. Detection by spraying with cobalt thiocyanate reagent (3 g ammonium thiocyanate and 1 g cobalt(II) chloride in 20 mL distilled water). The hRF value for imidacloprid was 82.