Rapid. Commun. Mass. Spectrom. 21, 3772-3776 (2007). A new fully automated online interface to couple HPTLC with ESI-MS/MS is presented for the first time. Among the major features of this interface are the time required for analysis, precision, suited for normal and reversed-phase layers and all plate sizes and carriers, no post-chromatographic process is required, it can be coupled universally with all LC-MS ion sources without any adjustment or mass spectrometer modification, and the quantitative analysis can be performed without any internal standard with a given detectability at the low-nanogram and even picogram level. The validation results for caffeine quantification in energy drinks and pharmaceutical samples, without internal standard, proved the reliability of the interface and its usefulness for quantitative analysis with comparable results to those obtained by validated HPTLC-UV methods.

J. Planar Chromatogr. 20, 49-51 (2007). HPTLC of mimosine (alpha-amino-3-hydroxy-4-oxo-1(4H)-pyridinepropanoic acid) on RP-18 with ethyl acetate - glacial acetic acid - water 60:10:17. Quantitation by densitometric scanning at 282 nm in absorbance mode. Limit of detection was 20 mg/g in the whole plant powder.

J. Chromatogr. A 1173 (1-2), 146-150 (2007). HPTLC of oenothein B and quercetin glucuronide in aqueous extract of Epilobii angustifolii herba on RP-18 W phase with 1) 25 % acetonitrile in water (with 50 mM H3PO4) over 80 mm for oenothein B, and 2) with acetonitrile over 40 mm for quercetin glucuronide. Quantification of oenothein B and quercetin glucuronide by densitometry at 270 and 350 nm, respectively. Linearity was between 1.14 and 2.28 µg/zone for oenothein B and 77 and 691 ng/zone for quercetin glucuronide. Aqueous extract of Epilobii angustifolii herba contained 152.46 ± 4.92 mg/g oenothein B and 22.07 ± 1.38 mg/g quercetin glucuronide.

59th Indian Pharmaceutical congress F-9, 392, (2007). HPTLC of sumatriptan succinate in bulk and tablet formulations on silica gel with methanol - water - glacial acetic acid 40:80:1. Densitometric evaluation at 230 nm.

J. Sep. Sci. 30, 1893-1898 (2007). HPTLC of L-DOPA in tablets on silica gel with acetone – chloroform – n-butanol – acetic acid glacial – water 12:8:8:8:7. Quantitative determination by absorbance measurement at 497 nm. The hRf value of L-DOPA was 37 and selectivity regarding matrix was given. Linearity was between 100 and 500 ng/µL. The intra-assay variation was between 0.26 and 0.65 % and inter-assay variation was between 0.52 and 2.04 %. The limits of detection and quantification were 1 and 3 ng/µL, respectively. No significant difference was found between this method and the official HPLC method.

59th Indian Pharmaceutical congress C-327, 303, (2007). HPTLC of 3-beta-23-24 -trihydroxy-olean-12-en-28-oic acid in Convolvulus pluricaulus on silica gel with n-hexane - ethyl acetate 7:4. Quantitative determination by densitometry at 226. The limit of detection and quantification was14.2 and 50 ng/zone, respectively. Recovery was 94.5 % - 97.2 %. The method was applied for analysis of herbal syrup and tablets.

J. Planar Chromatogr. 20, 203-207 (2007).. HPTLC of epalrestat (5-[(1Z,2E)-2-methyl-3-phenylpropenylidene]-4-oxo-2-thioxo-3-thiazolidineacetic acid) with nitrofurantoin as internal standard on silica gel with ethyl acetate - toluene - acetic acid 30:20:1. Densitometric scanning in absorbance mode at 290 nm.

59th Indian Pharmaceutical Congress C-42, 234, (2007). HPTLC of E-guggulsterone (a steroidal ketone present in oily resin of Commiphora mukul) and five marketed ayurvedic tablet formulations, on silica gel with toluene - propane-1-ol - glacial acetic acid 8:1:1. Densitometry at 254 nm. The hRf of E-guggulsterone was 61. Several marketed formulations were evaluated for E-guggulsterone, the UV spectra of the standard and that of the formulations were comparable in respect of E-guggulsterone. The TLC pattern showed several other zones corresponding to unknown phytochemicals. The method was suitable for quantitative evaluation of formulation in respect of E-guggulsterone in presence of other phyto chemicals.