Abstract No. F-237, 61st IPC (2009). HPTLC of tolterodine on silica gel with toluene - methanol - 25 % ammonia 250:250:1. The hRf value was 40. Quantitative determination by absorbance measurement at 284 nm. The method was linear in the range of 200-1000 ng/band. The compound was subjected to different stress conditions (acid, alkali, oxidation, thermal) and degradation under alkaline conditions was observed. The degradation products were well separated from the main component.

Abstract No. F-293, IPC (2009). HPTLC of aspirin and atorvastatin on silica gel with n-hexane - acetone - butyl acetate - formic acid 60:30:12. Quantitative determination by absorbance measurement at 242 nm. For both drugs, the method was linear in the range of 3-7 µg/band and the recovery was between 99.3 and 101.0 %.

Abstract No. F-263, 61st IPC (2009). HPTLC of repaglinide (REPA) and rosiglitazone (ROSI) on silica gel with benzene - methanol - acetone - acetic acid 80:10:9:1. Quantitative determination by absorbance measurement at 266 nm. Lienarity was in the range of 800-2800 ng/band and 400-2400 ng/band for REPA and ROSI, respectively. Recovery was 101.7 and 99.5 % for REPA and ROSI, respectively.

J. Sep. Sci. 32, 3239-3245 (2009). HPTLC of shikonin (1), acetylshikonin (2), and beta-acetoxyisovalerylshikonin (3) in four species of Arnebia on RP-18 with acetonitrile - methanol - 5 % formic acid 20:1:4. Quantitative determination by absorbance measurement at 520 nm. Linearity was in the range of 100-600 ng/zone for (1) and (2) and 100-1800 ng/zone for (3). The limits of detection for (1), (2) and (3) were 18, 15 and 12 ng/zone, respectively, while the limits of quantification were 60, 45 and 40 ng/zone, respectively.

J. Planar Chromatogr. 22, 367-369 (2009). HPTLC of triterpenoids (alpha- and beta-amyrin, oleanolic acid) on silica gel prewashed with methanol and dichloromethane, with dichloromethane - ethyl acetate 37:3. in a horizontal chamber saturated for 15 min. Detection by spraying with 8 % sulfuric acid in ethanol and heating at 105 °C for 3 min. Evaluation in daylight and under UV 366 nm. Quantitative determination by absorbance measurement at 520 nm.

Rapid Commun. Mass Spectrom. 24, 1032-1038 (2010) HPTLC of glycosphingolipids (GSLs) on silica gel with chloroform - methanol - water 120:70:17. The plate was overlaid with GSL-specific bacteria, and the microbes were detected with primary antibodies and appropriate alkaline phosphatase labeled secondary antibodies, and by in situ MS analysis of bacteria-specific GSL receptors. The thin-layer chromatography infrared matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry (TLC/IR-MALDI-o-TOF-MS) method represents one of the most powerful approaches for the detection of GSL receptors of microorganisms.

Rapid Commun. Mass Spectrom. 24, 1305-1311 (2010). HPTLC of salvinorin A (1), salvinorin C (2), divinatorin B (3), and salvinorin B (4) in the leaves of Salvia divinorium on silica gel with methyl tert-butyl ether – hexane 3:1 as mobile phase. Detection by desorption electrospray ionization multi-stage tandem mass spectrometry (TLC/DESI-MS). The hRf were 49, 64, 85, and 95 for compounds (1) - (4), respectively. The use of a simple HPTLC protocol in combination with DESI-MS allowed for improved detection of different species of salvinorin in the leaf extracts.

J. Ethnopharmacol. 124, 450-456 (2010). HPTLC fingerprinting of Eclipta alba on silica gel with chloroform - ethanol - water 35:10:2. Densitometric evaluation at 254 nm. Major compounds identified were coumestants and wedelolactone, with hair growth promoting activity.