J. Liq. Chromatogr. Relat. Technol. 28, 313-324 (2005). HPTLC of citalopram (1-[3-(dimethylamino)propyl]-1-(4-fluorophenyl)-1,3-dihydro-5-isobenzofurancarbonitrile) and moclobemide (as standard) on silica gel with benzene - acetone - ethanol - 25 % ammonia 9:8:2:1. Quantitative determination by densitometry at 226 nm and by video densitometry at UV 254 nm.

CBS 98, 9-11 (2007). Quantitative HPTLC of polycyclic aromatic hydrocarbons (PAH) from water samples, on caffeine-impregnated silica gel, with isopropyl acetate in a precooled (-20 °C, 30 min) twin-trough chamber without chamber saturation over 70 mm at -20°C. After application the dry plate was first equilibrated in the solvent-free trough for 10 min at -20 °C. Qualitative HPTLC at room temperature in the horizontal developing chamber with isopropyl acetate - n-hexane 3:1 over 50 mm. Detection by dipping in paraffin - toluene 1:1 (for fluorescence enhancement). Quantitative determination by fluorescence measurement at UV 366/>400 nm. Qualitative evaluation under UV 366 nm. The method is based on the German standard DIN 38407-7 for quantitative determination of 6 PAH but uses isopropyl acetate as a chlorine-free solvent instead of dichloromethane.

Food Chem. 95, 58-64 (2006). HPTLC of nicotinic acid (1) and pyrazole-3(5)-carboxylic acid (2) of Volvariella volvacea on silica gel with chloroform – methanol 17:3 with one drop of formic acid added. Quantitative determination by absorbance measurement at 190 nm for (1) and 262 nm for (2). The hRf values for (1) and (2) were 30 and 40, respectively. Linearity was between 400 and 7000 ng/zone (1) and 200 and 2500 ng/zone for (2). The limits of detection and quantification were 50 and 400 ng/zone for (1) and 20 and 200 ng/zone for (2). Recoveries of both compounds were between 96 and 102 %.

J. Sep. Sci. 30, 2092-2096 (2007). HPTLC of flavonoids in Bauhinia variegata, Bacopa monnieri, Centella asiatica, Ginkgo biloba, Lonicera japonica, Rosa bourboniana, Rosa brunonii, and Rosa damascena on RP-18 with two-fold development with water (5 % formic acid) – methanol 7:3 and water (5 % formic acid) – methanol 1:1 as mobile phases. Quantitative determination by absorbance measurement at 280 nm. The hRf values of apigenin (1), quercetin (2), rutin (3), luteolin (4), and quercitrin (5) were 19, 29, 34, 51, and 63, respectively. Linearity was between 150 and 800 ng/zone for (1) and (3) and between 200 and 1000 ng/zone for (2), (4) and (5). The limits of detection and quantification for (1) – (5) were 30 and 166 ng/zone, 40 and 200 ng/zone, 20 and 150 ng/zone, 40 and 200 ng/zone, and 40 and 200 ng/zone, respectively. Recovery was between 97 and 99.8% for (1) – (5).

59th Indian Pharmaceutical congress F-220, 442, (2007). HPTLC of 6-gingerol in herbal extracts on silica gel with n-hexane - ethyl acetate - ammonia 14:5:1 in a chamber saturated for 45 min. Densitometric evaluation at 254nm. The hRf value of 6-gingerol was 52. The method was linear in the range of 100 - 1200 ng/zone.

Chromatographia 66 (1-2), 95-102 (2007). HPTLC of dasatinib in the presence of its degradation products, on silica gel sheets with toluene - chloroform 7:3. Quantification by densitometry at 280 nm. The hRf value of dasatinib was 23 and selectivity regarding matrix was given. Validation of the method as per the ICH guidelines. Dasatinib was subjected to acid–alkali hydrolysis, oxidation, dry heat, wet heat and photodegradation. The drug was susceptible to acid–alkali hydrolysis and oxidation. The drug was found to be stable in neutral, wet heat, dry heat and photo-degradation conditions.

CBS 98, 2-4 (2007). HPTLC of amino-3-propan-1-ol, a degradation product of dexpanthenol, on silica gel with ethanol - water - acetic acid 16:3:1. To the mobile phase the derivatization reagent was added, i.e. 0.5 g ninhydrin were dissolved in 100 mL solvent mixture. Development in the horizontal developing chamber from both plate sides over 40 mm. Detection by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 486 nm. The hRf value of amino-propanol was 50. The mean repeatability was 4.9 % at 5 different concentration levels. The relative standard deviation of the intermediate precision (n=9) was 5.7 %. The limit of detection and quantification was 4.5 µg/mL and 15 µg/mL, respectively (related to the application volume of 2 µL). Recovery (n=15) was 102 %.

iNDIAN dRUGs 44(8), 632 (2007). TLC, HPTLC and NMR spectroscopic methods are reported for identification and characterization of aloin isolated from Kumariasava and Aloe vera. TLC and HPTLC of chloroform extracts on silica gel with chloroform - ethyl acetate 3:1. Detection under UV 366 nm. The hRf value of aloin was 84.