59th Indian Pharmaceutical congress F-152, 426, (2007). HPTLC of rosiglitazone on silica gel aluminum layer with chloroform - ethyl acetate - ammonia 70:30:1. The hRf value of rosiglitazone was 40. Densitometric analysis in absorbance mode at 230 nm. Linearity was between 100 and 1000 ng/zone. Recovery (by standard addition method) was 99.6 %. Limit of detection and quantification was 10 ng and 50 ng/zone, respectively.

59th Indian Pharmaceutical congress F-224, 443, (2007). HPTLC of cilostazol on silica gel with ethyl acetate - toluene - methanol - ether 4:4:2:1. The hRf value was 64. Limit of detection and quantification was 7 ng/spot and 20 ng/spot respectively.

CBS 97, 12-15 (2006). HPTLC of flavonoids from green tea (Camellia sinensis) on silica gel in a saturated twin-trough chamber with ethyl formate - toluene - formic acid - water 60:3:8:6. Detection by dipping the hot plate (heated at 100 °C for 2 min) into natural products reagent, followed by drying, dipping into polyethylene glycol 400 (10 g in 200 mL dichloromethane), and drying. Evaluation under UV 366 nm. With this method the geographical origin of the material can be determined. Toluene - acetone - formic acid 9:9:2 allows the discrimination of green from black and other speciality teas, based on the polyphenol pattern. Detection by dipping the hot plate into a solution of Fast Blue Salt B. Evaluation under white light. For investigation of the alkaloid profile ethyl acetate - methanol - water 20:2.7:2 and evaluation under UV 254 nm is used. The amino acid profile is analyzed by using 1-butanol - acetone - acetic acid - water 7:7:2:4. Detection by dipping in ninhydrin reagent, followed by heating at 110 °C for 3 min. Evaluation under white light.

Indian Drugs 44(10), 751 (2007). TLC and HPTLC of methanolic extracts of Eclipta alba, Launaea nudicaulis and Tridax procumbens, on silica gel with toluene - ethyl acetate - methanol 14:5:1. Evaluation under 254 and 366 nm. Detection by spraying with anisaldehyde sulfuric acid followed by evaluation at 525 nm.

Phytochem. Anal. 19, 2-16 (2008). Recent developments in the phytochemical analysis of Panax ginseng are described, including different approaches such as the determination of the total saponin content and target compound and group-specific analysis using HPTLC-MS. In metabolite profiling, the paper describes the use of nuclear magnetic resonance spectroscopy and high-resolution mass spectrometry.

J. Food Comp. Anal. 21, 496-500 (2008). HPTLC of bergenin (1), catechin (2), and gallic acid (3) from Bergenia ciliata and Bergenia ligulata on silica gel with toluene - ethyl acetate - formic acid 4:6:1. Quantitative determination by absorbance measurement at 280 nm. The hRf values of (1), (2), and (3) were 29, 54, and 60, respectively. Selectivity regarding matrix was given. Linearity was between 160 and 800 ng/spot for (1), 160 and 480 ng/spot for (2), and 160 and 560 ng/spot for (3). The limits of detection and quantification were 120 and 160 ng for (1), 80 and 120 ng for (2), and 40 and 80 ng for (3), respectively. Recovery was 99.3 % for (1), 98.6 % for (2), and 99.2 % for (3). The intermediate/interday/intra-day precision (n=6) was 0.04 % and 0.07 % for (1), 0.07 % and 0.06 % for (2), and 0.02 % and 0.11 % for (3).

Indian Drugs 45 (3), 233-225 (2008). HPTLC of atisine in Aconitum heterophyllum on silica gel with toluene - ethyl acetate - diethylamine 7:2:1. Densitometric evaluation at 274 nm. Detection by spraying with Dragendorff reagent followed by spraying with 10 % sodium nitrate. Densitometric evaluation of reddish brown zones at 520 nm. Linearity of atisine was between 10 and 60 ng/spot.

J. Sep. Sci. 31, 71-77 (2008). HPTLC of acrylamide in drinking water on silica gel, derivatization in situ with 5-dimethylamino-naphtalene-1-sulfinic acid (1.6 µg/µL in methanol), followed by heating at 120 °C for 1 hour and developed with ethyl acetate. For fluorescence enhacement, the plate was dipped into a solution of 25 % polypropylene glycol in n-hexane and dried immediately. Quantitative determination by fluorescence at 366/>400 nm. Verification was based on HPTLC-ESI/MS, HPTLC-direct analysis in real time (DART)-TOF/MS and NMR. The hRf value of acrylamide (as 3-dansylpropanamide) was 69. Linearity was between 0.1 and 0.4 µg/L. Within-run precision and the mean between-run precision (n=3) were 4.6 and 11.0 %. The limit of detection and quantification for acrylamide was 0.025 and 0.083 µg/L, respectively. Recovery (by standard addition) was 96.4 %. The method showed comparable result with HPLC-MS/MS.