J. Ethnopharmacol. 194, 30-56 (2016). Review of information on the ethnobotany, phytochemistry, pharmacological research and toxicity of Anogeissus species, including the application of HPTLC for the quantitative investigation of gallic acid, ellagoc acid as well as flavonoids like quercetin and rutin.

growing in Nilgiri hills of India. J. Planar Chromatogr. 29, 347-355 (2016). HPTLC of forskolin in the roots of Coleus forskohlii on silica gel with toluene – ethyl acetate – methanol 180:60:1. Detection by dipping into anisaldehyde ‒ sulfuric acid reagent. Quantitative determination by absorbance measurement at 545 nm. The hRF value for forskolin was 48. Linearity was between 20 and 100 ng/zone. The intermediate precisions were below 1.6 % (n=3). The LOD and LOQ were 1 and 3 ng/zone, respectively. Recoveries were between 98.3 and 101.5 %.

J. of Chromatogr. A 1441, 126-133 (2016). Presentation of a method for the analysis of ergot alkaloids including an ammonium acetate buffered extraction step, followed by a fast liquid-liquid partitioning pre-cleaning and then planar solid phase extraction (pSPE). HPTLC on amino phase with methanol for separation of the ergot alkaloids from the remaining matrix and for focusing them in a single zone. Quantification after dipping the plate in n-hexane – paraffin solution for fluorescence enhancement. The LOD and LOQ was 0.07 and 0.24 mg/kg rye, respectively, expressed as ergocristine, which was well below the currently applied quality criteria limit for rye. The recovery was almost 100 % at relevant spiking levels for different rye flour samples. The pSPE–FLD method was fast, efficient and reliable for screening the total ergot alkaloid content in rye and it was a rapid alternative to the HPLC determination with summing up the individual alkaloids. Furthermore, HPTLC-MS additionally enables the identification of the ergot alkaloid composition by a single mass spectrum, when utilized as a fingerprint, offering an easy differentiation of Secale cornutum from different origins.

J. Planar Chromatogr. 29, 366-371 (2016). HPTLC of the diastereomeric amides of (RS)-etodolac on silica gel with acetonitrile – methanol – dichloromethane – water 12:2:2:1. Detection by exposure to iodine vapor. The hRf value for the first and second eluting diastereomers were 22 and 56, respectively.

J. Planar Chromatogr. 30, 106-112 (2017). RP-HPTLC (1) and micellar liquid chromatography-TLC (2) of 1,2,4-triazole derivatives on RP-18 with methanol and acetonitrile in volume range concentration between 0.3 and 0.65 organic modifier for (1) and sodium dodecyl sulfate concentrations above critical micellar concentration in the range between 0.0318 and 0.1318 mol/L for (2). Detection by exposure to iodine vapors at room temperature. Developments were performed in moderate (≈0.4 T) magnetic field and simultaneously outside it. The partition coefficient P value of the investigated substances was used as a descriptor of their lipophilic properties.

and Cassia angustifolia Vahl. J. Planar Chromatogr. 30, 238-244 (2017). HPTLC fingerprint of sennoside A and B in Cassia senna L. and Cassia angustifolia on silica gel with 1-propanol ‒ ethyl acetate ‒ water 4:4:3. Detection by heating at 110 °C for 10 min, followed by spraying with 50 g/L potassium hydroxide in ethanol 50 % and heating again at 110 °C for 10 min. Qualitative determination at UV 366 nm.

J. Ethnopharmacol. 197, 184-194 (2017). HPTLC of arjunetin in Terminalia arjuna on silica gel with ethyl acetate – toluene – formic acid – acetic acid 12:6:1:2. Detection by spraying with anisaldehyde sulfuric acid reagent. The hRF value for arjunetin was 25.

J. Planar Chromatogr. 30, 291-298 (2017). HPTLC of cefuroxime axetil and cefepime in human whole blood and urine on silica gel with chloroform – ethyl acetate – glacial acetic acid – water 4:4:1:3 for (1) and ethanol – 2-propanol – glacial acetic acid – water 4:4:1:3 for (2). Quantitative determination at UV 285 nm for (1) and 266 nm for (2). The hRF values for (1) and (2) were 89 and 21, respectively. Linearity was between 3-77 μg/mL for (1) and 3-38 μg/mL for (2). The intermediate precision (n=6) was <2 % for (1) and (2). LOD and LOQ were in the range of 40 and 470 ng/zone. Recovery rate ranged from 95.8 to 101.5 % for (1) and (2).