J. Planar Chromatogr. 29, 281-286 (2016). HPTLC of newly synthesized cycloalkylspiro-5-hydantoins (derivates of 3-(4-substituted benzyl)-cyclopentanespiro-5-hydantoin, 3-(4-substituted benzyl)-cyclohexanespiro-5-hydantoin, and 3-(4-substituted benzyl)-_x000D_cycloheptanespiro-5-hydantoin) on RP-18 with varying content of methanol 50–90%. Multivariate analysis enabled the classification of the lipophilicity and structural properties of the investigated compounds.

J. Planar Chromatogr. 29, 318-322 (2016). HPTLC of trans-resveratrol, aromatic acids (benzoic, cinnamic, ferulic, salicylic and caffeic acid) and flavones (flavone, daidzein, chrysin, kaempferol, quercetin and morin) in coffee, tea and wine on LiChrospher® plates with ethyl acetate – cyclohexane – 1-butanol 9:9:2 for trans-resveratrol and ethyl acetate – methanol – water 77:13:10 for aromatic acids and flavones. Chemiluminescence was induced by dipping the plate into a bis(2,4,6-trichlorophenyl)oxalate (250 mg in 36 mL 1-butyl acetate followed by shaking with 0.4 mL hydrogen peroxyde 35 % for 20 min) solution for 1 s. Fluorescence evaluation under UV 366 nm.

J. Liq. Chromatogr. Relat. Technol. 39, 322-324 (2016). HPTLC of neutral (1) and polar lipids (2) in Biomphalaria glabrata snails on silica gel with petroleum ether – diethyl ether – glacial acetic acid 80:20:1 for (1) and chloroform – methanol – water 130:50:9 for (2). Detection of neutral lipids by spraying with 5 % phosphomolybdic acid in absolute ethanol and polar lipids using 10 % copper (II) sulfate in 8 % phosphoric acid.

J. Liq. Chromatogr. Relat. Technol. 39, 312-321 (2016). HPTLC of lupeol in waxes of different cabbage varieties on RP-18 with ethyl acetate – acetonitrile 5:1. Detection by dipping into the anisaldehyde reagent (16 mL sulfuric acid were dropwise added to a cooled mixture of 20 mL glacial acetic acid and 170 mL methanol, then 1 mL of anisaldehyde was added), followed by heating at 110 °C for 30 s. Quantitative determination by absorbance measurement at 535 nm. The hRF of lupeol was 40. Linearity was in the range of 30-150 ng/zone. The LOD and LOQ were 7 and 20 ng/zone, respectively. Intermediate precisions were below 10 %. Recoveries were between 90.3 and 93.1 %.

J. Planar Chromatogr. 29, 273-280 (2016). HPTLC of piroxicam, tenoxicam, meloxicam and isoxicam on silica gel with ethyl acetate – toluene – butylamine 2:2:1. Detection under UV 290 nm. The method was applied to study the effect of oxidizing or reducing agents on the stability of these drugs.

J. Planar Chromatogr. 29, 176-183 (2016). HPTLC of atracurium besylate (1) and atropine sulfate (2) on silica gel with acetonitrile – methanol – dichloromethane – glacial acetic acid – water containing 70 mg L-(+)-tartaric acid 70:10:5:7:1, pH 5 for (1), and methanol – water containing 40 mg L-histidine and 20 mg copper(II) acetate 22:3, pH 7 for (2). Quantitative determination by absorbance measurement at 280 nm for (1) and 215 nm for (2). The hRF values for (1) and (2) were 51 and 65, respectively. Linearity was in the range of 2-14 μg/zone for (1) and 5-35 μg/zone for (2). Intermediate precisions were below 1 %. The LOD and LOQ were 0.5 and 1.6 µg/zone for (1) and 1.2 and 3.6 µg/zone for (2). Average recovery was found to be 103.4 % for (1) and 96.6 % for (2).

J. Ethnopharmacol. 192, 283-291 (2016). Ubtan is a traditional herbal formulation in the Indian system of medicine, prepared by mixing powdered turmeric (Curcuma longa L.), chickpea (Cicer arietinum L.) and sandal wood (Santalum album L.). HPTLC of four in-house formulations of Ubtan (UF-1, UF-2, UF-3 and UF-4, prepared with varying quantities of each ingredient) on silica gel with cyclohexane – ethyl acetate – formic acid 90:9:1. Detection by spraying with p-anisaldehyde sulfuric acid reagent, followed by drying at 40 ºC for 10 min. The hRF values for UF-1 were 70 and 42.

Food Res. Int. 88, 263-275 (2016). TLC of residual triacylglycerides, diacylglycerides, monoacylglycerides and free fatty acids in raw and pasteurized human milk on silica gel with heptane – diethyl ether – acetic acid 55:45:1, followed by drying at 150 °C for 15 min. Quantitative determination by flame ionization._x000D_