Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

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      106 109
      A simplified method for rapid quantification of intracellular nucleoside triphosphates by one-dimensional thin-layer chromatography
      CH. JENDRESEN*, M. KILSTRUP, J. MARTINUSSEN (*Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark, 2800 Lyngby, Denmark)

      Anal. Biochem. 409 (2), 249-259 (2011). Presentation of a less time-consuming, more sensitive, and more precise method for the quantitative determination of nucleoside triphosphates (NTPs), 5-ribosyl-1-pyrophosphate (PRPP), and inorganic pyrophosphate (PPi) in cell extracts by TLC: Separation of an acid extract of L. lactis by charcoal filtration into a filtrate and an eluate, which then was separated by TLC either in the Cashel solvent (0.85 M potassium phosphate, pH 3.4) or in the AFC solvent (3 M ammonium formate [pH 2.4] and 0.7 M ammonium chloride). Two-dimensional separation of 18 µL of the eluate sample using the AFC solvent in the first dimension and using 0.75 M LiCl in 7.5 % lithium borate (pH 6.8, borate solvent) in the second dimension.

      Classification: 21
      106 132
      Enhanced quantitative evaluation of the HPTLC-bioluminescence detection
      Vera BAUMGARTNER*, W. SCHWACK (*State Laboratory of the Canton Basel-City, Kannenfeldstrasse 2, 4056 Basel, Switzerland; vera.baumgartner@bs.ch)

      J. Liq. Chromatogr. Relat. Technol. 33, 980-995 (2010). HPTLC of bronopol and Kathon CG on silica gel (pre-washed with methanol) with methanol - dichloromethane - n-hexane 2:12:7. For detection, luminescent Vibrio fischeri bacteria were cultivated. Plates were dried for 30-40 min at 120 °C on a plate heater and then immersed for 1 s in the bacteria suspension using an immersion device and a glass dip tank. Excess bacteria suspension was removed. Images were taken using the BioLuminizer with an exposure time of 55 s. The effective and reasonably fast quantitative method is in routine use at several laboratories.

      Classification: 29f
      106 158
      Rapid validated HPTLC method for estimation of betulinic acid in Nelumbo nucifera (Nymphaeaceae) rhizome extract
      D. MUKHERJEE, N. KUMAR, T. KHATUA, P. MUKHERJEE* (*School of Natural Products Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India, pulokm@gmail.com)

      Phytochem. Anal. 21, 556-560 (2010). HPTLC of betulinic acid in the rhizomes of Nelumbo nucifera on silica gel with chloroform - methanol - formic acid 49:1:1. Detection by spraying with anisaldehyde - acetic acid - methanol - sulfuric acid 5:100:850:50 and drying at 50 °C for 10 min. Quantitative determination by absorbance measurement at 420 nm. The hRf of betulinic acid was 30. Linearity was between 2 and 10 µg/zone. Detection and quantification limits were 0.4 and 2.30 µg, respectively. The intra-day and inter-day precisions were 0.4 % and 0.3 % (n=3) respectively. Recovery (by standard addition) was 98.4 %.

      Classification: 32e
      107 005
      Thin layer chromatography in helminthology
      B. FRIED*, J. SHERMA (*Lafayette College, Department of Chemistry, Easton PA 18042-1782, USA)

      Revista Iberica de Parasitologia 65 (1-4), 21-36 (2005). Review on the TLC literature in helminthology from 1996 to 2004. Principles and practices of modern TLC for the analysis of lipids, amino acids, carbohydrates and pigments in helminths such as various species of trematodes, cestodes and nematodes are described.

      Classification: 1
      107 033
      HPLC-MS or simply HPTLC for analysis of sucralose in water?
      S. GRASHORN, L. SCHUELE, Gerda MORLOCK* (*University of Hohenheim, Institute of Food Chemistry, Garbenstrasse 28, 70599 Stuttgart, Germany, gerda.morlock@uni-hohenheim.de)

      CBS 106, 7-10 (2011). HPTLC of sucralose on silica gel (pre-washed by development with methanol, followed by drying at 100 °C for 15 min) with isopropyl acetate – methanol – water 15:3:1 up to 60 mm (migration time 15 min). Detection by dipping in aniline diphenylamine o-phosphoric acid reagent followed by heating at 120 °C for 20 min, evaluation under white light and UV 366 nm. Quantitative determination by absorbance measurement at 400 nm. Via the TLC-MS Interface the respective zones were eluted and transferred into a single-quadrupole mass spectrometer. Electrospray ionization mass spectra were recorded in full scan mode. The recovery of sucralose in drinking water was 84 ± 7 % (n=3). The limit of detection was 6 ng/band. The calibration curve (10-300 ng/band, r=0.9999, 1.3 %RSD) was suited to analyze sucralose at concentrations of 0.1-5 µg/L.

      Classification: 5c
      107 102
      RP-HPTLC determination of the lipophilicity of some new derivatives of thiosemicarbazide and 1,2,4-triazole of sulphanylacetic acid
      A. HAWRYL*, L. POPIOLEK, M. DOBOSZ, E. PIKULA, M. WAKSMUNDZKA-HAJNOS (*Med. Univ. of Lublin, Dep. of Inorg. Chem., Staszica 6, 20-081 Lublin Poland)

      Acta Chromatographica 22 (1), 37-55 (2010), DOI:10.1556/AChrom.22.2010.1.3. Separation of some new derivatives of thiosemicarbazide and the 1,2,4-triazole of sulphanylacetic acid by HPTLC on RP-18 with mobile phases containing water and an organic modifier (methanol, dioxane, acetone, 2-propanol, or tetrahydrofuran). Description of the relationships between solute retention and modifier concentration by Snyder’s linear equation. RM0 and slope values were determined by extrapolation based on linear retention and mobile phase concentration; both values characterize the lipophilicity of the substances. Correlation of the calculated values of RM0 with log P values for the drugs investigated by use of the software HyperChem, and correlations between intercept (RM0) and slope from the linear equations.

      Keywords: HPTLC
      Classification: 24
      107 136
      Simultaneous analysis of atorvastatin calcium and losartan potassium in tablet dosage forms by RP-HPLC and HPTLC
      H.J. PANCHAL*, B.N. SUHAGIA (*Shree S.K. Patel College of Pharm. Educ. & Research, Ganpat Vidyanagar, Kherva, Mehsana 382711 Gujarat, India)

      Acta Chromatographica 22 (2), 173-187 (2010), DOI:10.1556/AChrom.22.2010.2.2. HPTLC on silica gel with methanol – carbon tetrachloride – ethyl acetate – glacial acetic acid 80:636:280:4. The hRf values were 45 and 30 for atorvastatin calcium and losartan potassium, respectively. Quantification by densitometry at 238 nm. Linearity was in the range of 50–500 ng/band for each substance. The recoveries were 100.6 % and 100.5 % for atorvastatin calcium and losartan potassium, respectively. No interference from excipients was observed. The results were compared statistically using a paired t-test with results by an RP-HPLC method. Both methods provided comparable results.

      Classification: 32e
      108 009
      Normal and reversed phase thin layer chromatography data in quantitative structure-activity relationship study of compounds with affinity for serotonin (5-HT) receptors
      Grazyna ZYDEK*, Elzbieta BRZEZINSKA (*Dep. of Anal. Chem., Med. Univ. of Lodz, Muszynskiego 1 Street, 90-151 Lódz, Poland)

      J. of Chromatogr. B 879, 1764-1772 (2011). Quantitative structure-activity relationship (QSAR) analysis of 20 drugs with affinity for serotonin (5-HT) receptors by calculation of a set of physicochemical parameters and TLC data. TLC on silica gel and RP-2 impregnated with solutions of aspartic acid, serine, phenylalanine, tryptophan, tyrosine, asparagine, threonine and their mixtures, with two mobile phases - the systems were chosen as models of drug-5-HT-receptor interaction. The relationships between chromatographic data, molecular descriptors and biological activity data were found by regression analysis. The resulting correlations for the compounds with serotoninergic activity represent their interaction with the proposed biochromatographic models. The presented regression models based on biochromatographic studies can be an efficient tool in the QSAR analysis for initial prediction of compounds activity direction within 5-HT receptors.

      Classification: 2
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