J. Planar Chromatogr. 27, 204-209 (2014). HPTLC of naringin in the leaves and stems of Rumex vesicarius on silica gel with ethyl acetate - glacial acetic acid - methanol - water 30:10:5:1. Quantitative determination by absorbance measurement at 275 nm. The hRF value for naringin was 46. Linearity was in the range of 100-1000 ng/zone. The intermediate/interday/intra-day precisions were below 2 % (n=6). The LOD and LOQ were 37 and 111 ng/zone, respectively. The average recovery was 99.5 %.

J. Planar Chromatogr. 27, 42-46 (2014). HPTLC of beta-sitosterol in the root cultures of Clitoria ternatea on silica gel with n-hexane - acetone 4:1. Detection by dipping into 5 % methanol - sulfuric acid reagent, followed by heating at 105 ºC for 3 min. Quantitative determination by absorbance measurement at 414 nm. The hRF value for beta-sitosterol was 31. Linearity was in the range of 100-500 ng/zone. The intermediate/interday/intra-day precisions were below 2 % (n=3). The LOD and LOQ were 40 and 100 ng/zone, respectively. Recovery was between 97.2 and 98.5 %.

Phytochemistry 105, 68-78 (2014). HPTLC of antifungal compounds in Morinda tormentosa on silica gel with dichloromethane - methanol - ethyl acetate 43:6:1. Bioautography with Candida albicans DSY2621 or parent wild type CAF2-1 at 37 ºC overnight, followed by spraying with an aqueous solution (2.5 mg/ml) of thiazolyl blue (methyl thiazolyl tetrazolium chloride) and incubation for 6 h at 37 ºC. Clear inhibition zones were observed against a purple background.

J. Planar Chromatogr. 27, 267-273 (2014). HPTLC of kaempferol (1), rutin (2), and quercetin (3) in marketed formulations containing_x000D_ Phyllanthus niruri (Bhuiamla) and Emblica officinalis (Amla) on silica gel with chloroform - methanol - formic acid 82:15:10. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) to (3) were 69, 53 and 12. Linearity was in the range of 21-840 ng/zone for (1), 56-1008 ng/zone for (2) and 60-1800 ng/zone for (3), respectively. The intermediate/interday/intra-day precisions were below 1 % (n=3). The LOD and LOQ were 21 and 63 ng/zone for (1), 56 and 168 ng/zone for (2) and 300 and 600 ng/zone for (3), respectively. Recoveries for (1) to (3) were obtained in the range of 81.8-119.0 %.

J. Liq. Chromatogr. Relat. Technol. 37, 2784-2799 (2014). HPTLC of atorvastatin (1), ezetimibe (2) and fenofibrate (3) on silica gel with toluene – methanol – triethylamine 16:3:1. Quantitative determination by absorbance measurement at 245 nm. The hRF values for (1) to (3) were 10, 20 and 80, respectively. Linearity was in the range of 100-350 ng/zone for (1) and (2) and 1600-5600 ng/zone for (3). The intermediate/interday/intra-day precisions were below 1 % (n=3). The LOD and LOQ were 60 and 90 ng/zone for (1), 80 and 100 ng/zone for (2) and 90 and 150 ng/zone for (3), respectively. Recoveries were in the range of 97.6-102.6 %.

J. Chromatogr. A 1381, 229-238 (2015). TLC screening and TLC-MS2 analysis of triterpenoids and phytosterols on (A) RP-18 with ethyl acetate - acetonitrile 3:2; (B) RP-18 with with acetone - acetonitrile 5:1; (C) silica gel with n-hexane - ethyl acetate 5:1. Detection of (A), (B) and (C) by dipping with a methanolic solution of anisaldehyde reagent, followed by heating at 110 °C for 30 s. Qualitative identification under white light and at UV 366 nm. The TLC-MS Interface was used for the elution of compounds from the plates into a LCQ ion trap system equiped with atmospheric pressure chemical ionization in positive mode. Acetonitrile was used as an eluent at a flow rate of 0.3 mL/min. This approach of combination TLC and MS enabled positive assignment of different triterpenoids and phytosterols.

J. Planar Chromatogr. 28, 42-47 (2015). HPTLC of (1) homoeriodictyol and (2) persicogenin in the methanol extract of the aerial parts of Rhus retinorrhoea and Rhus tripartita on silica gel with toluene - ethyl acetate - methanol 16:4:1. Quantitative determination by absorbance measurement at 293 nm. The hRF values of (1) and (2) were 30 and 48. Linearity was between 100 and 800 ng/zone for both, (1) and (2). The intermediate intra-day and inter-day precisions were below 2 % (n=6). The LOD and LOQ were 26 and 77 ng/zone for (1) and 31 and 92 ng/zone for (2), respectively. Recoveries were in the range of 98.9-99.5 % for (1) and 98.3-99.2 % for (2).

Pharmazie 68, 534-540 (2013). Detection and quantification by instrumental HPTLC and 1H-qNMR analyses. HPTLC of stachydrine on silica gel with methanol - dichloromethane - 25 % ammonia 8:2:3 with chamber saturation for 10 min in the horizontal developing chamber. The developing distance was 8.5 cm. Derivatization by dipping in Vagujfalvi solution (Wagner et al. 1983). Stachydrine was detected as red zone on yellow background. Quantitative determination by absorbance measurement at 517 nm. The method was selective with no other Dragendorff-positive constituents detected between hRF 45 - 60. The LOD was below 250 ng/zone. The results for the stachydrine content in the samples evaluated via peak height and peak area were in high accordance with independently performed 1H-qNMR measurements.