Abstract G-9, IPC (2005). HPTLC of pantoprazole and mosapride in combined dosage form on silica gel with methanol - toluene - chloroform 4:30:15. Quantitative determination by absorbance measurement at 305 nm. The hRf value of pantoprazole was 31 and of mosapride 43, recovery was 99.9-101.1 %. Accuracy, precision, and linearity of the method were established.

Abstract G-33, IPC (2005). HPTLC of epalrestat in plasma on silica gel with ethyl acetate - toluene - acetic acid 30:20:1. Nitrofuranloin was used as internal standard. Quantitative determination by absorbance measurement at 390 nm. Linearity was obtained the range of 0.01-0.20 µg/mL with recovery of 99-107 %. The method was validated as per ICH guidelines.

J. Planar Chromatogr. 18, 194-198 (2005). HPTLC of rosuvastatin calcium (with aceclofenac as internal standard) on silica gel with toluene - methanol - ethyl acetate - formic acid 60:10:30:1. Quantitative determination by absorbance measurement at 265 nm. A good determination coefficient (r2 = 0.9999) was obtained for the linearity in the range of 1.0 to 15.0 µg of sample. For formulation and bulk drug the mean percentage assay was 100.09 +/- 0.20 and 100.07 +/- 0.48, respectively. The accuracy of the method was found to be 100.62 % and precision was found to vary from 0.01 to 0.77 %.

J. Planar Chromatogr. 19, 92-97 (2006). Two-dimensional HPTLC of naringenin, acacetin, flavone, morin, hesperetin, quercetin, narcisin, kaempferol 3,7-dirhamnoside, naringin, rutin, astragalin, quercitrin, kaempferol 3-glyco-7-rhamnoside, naringenin 7-glucoside, ferulic acid, chlorogenic acid, elagic acid, caffeic acid, p-coumaric acid, m-coumaric acid, o-coumaric acid, and gallic acid by connecting diol or silica plates to RP18 plates, with e. g. acetone - water 2:3 or 1:1 in first direction of development and propan-2-ol - ethyl acetate 1:1 or methanol - ethyl acetate 1:9 in second direction of development. Derivatization by use of diphenylboric acid 2-aminoethylester (natural products reagent), followed by PEG 400 reagent; detection under 365 nm.

J. Chromatogr. A 1112 (1-2), 165-170 (2006). HPTLC of a herbal medicinal product containing 250 mg of Aesculus hippocastanum dry extract, 120 mg of Vitis vinifera dry extract and 50 mg of excipients. After purification with C18 SPE cartridges, HPTLC on silica gel with the upper layer of a mixture of acetic acid - water - butanol 1:4:5. Detection by spraying with anisaldehyde reagent followed by heating the plate for 5-10 min at 100-105 °C. Quantitative determination by measurement at 535 nm. The method was developed to analyze the total saponin content (also referred to as the aescin content) and is applicable for the quality control and stability investigation of both the Aesculus dry extract and HMP capsules thereof containing Vitis dry extract in combination with the Aesculus dry extract. The method was validated according to the International Conference on Harmonization (ICH) guidelines. The proposed assay method is specific for aescin in the presence of Vitis dry extract and formulation excipients. Analysis of stressed samples in forced degradation tests proves the method to be applicable for stability evaluation. The standard aescin curve is linear (r > 0.99) over a concentration range of 0.16–0.80 µg/spot. Recovery from the HMP capsules is statistically equal to 100 %. The precision of the method with respect to time and concentration is acceptable, with relative standard deviation values of 1.28 and 1.49 %, respectively.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (7), 854-856 (2005). TLC of chenodeoxycholic acid in Hedan tablets on silica gel with n-hexane - ethyl acetate - acetic acid - methanol 20:25:2:3. Detection by spraying with 10 % H2SO4 in ethanol followed by heating at 105 ºC until the spots are visualized. Quantification of chenodeoxycholic acid by densitometry at 375 nm. Validation of the procedure by investigation of its linearity range (0.47 - 2.33 µg/spot, R = 0.9992); of its repeatability (3.3 %, n = 5); of its precision (3.9 %, n = 5 within plate and 4.7 % n= 5 plate-to-plate); and its standard addition recovery (98.4%, RSD = 2.5 %, n = 5).

J. Planar Chromatogr. 19, 129-134 (2006). HPTLC of enrofloxacin, oxytetracycline, and trimethoprim on cyano phases with 0.5 M oxalic acid - methanol (5:5; 6:4; 7:3; 8:2). Detection under UV light at 254 nm. Quantitation by videodensitometry at 254 nm.

J. Chil. Chem. Soc. 48, 19-23 (2003). HPTLC validation of atrazine in aqueous extracts of soils on silica gel (layer thickness 100 µm) previously activated at 120 ºC for 30 min. The elution program applied to aqueous soil matrices started with 10 short isocratic runs (0.8 min) with acetonitrile – dichloromethane 30:70. Mixer was emptied after the tenth step and refilled to continue with 4 successive isocratic runs (2.5, 5.0, 7.5 and 25 min) with dichloromethane. The plate was dried for 1 min between each step and for 3 min after the last one. The plate was preconditioned with nitrogen for 15 s before each run. Quantitative determination by absorbance at 210 nm. Linearity is between 5 and 100 ng and recoveries ranging from 98.7 to 103.5 %. The detection limit is 1.5 ng and the quantification limit is 4.9 ng. Precision analysis shows an intra-assay variation between 1.48 and 5.47 %. The method can be applied to a broad range of soils including those with high organic matter content.