Ind. J. Pharm. Res. 6 (4), 205-207 (2007). HPTLC of itraconazole on silica gel with toluene - acetone - triethylamine 30:30:1. Detection at 270 nm. The hRf value of itraconazole was 62. Linearity was between 0.2 and 0.6 µg. The market formulation contained itraconazole amounts of 99.6 % via peak height and 100.2 via peak area, respectively. Recovery (by standard addition) was 99.4 % and 100.3 % respectively.

J. Chromatogr. Sci. 46 (4), 304-307 (2008). HPTLC rabeprazole and domperidone in pure powder and in capsule formulations on silica gel aluminium layers with ethyl acetate - methanol - benzene - acetonitrile 3:2:3:2. Quantification by absorbance measurement at 287 nm. Linearity was between 400 and 1200 ng/spot for rabeprazole and 600 and 1800 ng/spot for domperidone. Recovery was 99.8 % for rabeprazole and 99.4 % for domperidone. This method is simple, precise, and sensitive, and it is suitable for the simultaneous determination of rabeprazole and domperidone.

CBS 99, 14-15 (2007). HPTLC of acrylamide prechromatographically derivatized with dansulfinic acid on silica gel in a twin-trough chamber with ethyl acetate over 70 mm after focussing with methanol. Detection by dipping in a 25 % solution of propylene glycol in n-hexane. Quantitative determination by fluorescence measurement at 366/>400 nm. Precision (RSD, n=3 at 3 different concentration levels each) was 4.8 % and recovery (by standard addition) was 96 %. Linearity was between 0.1 and 0.4 µg/L. The limit of quantification was 0.08 µg/L acrylamide in drinking water. Comparison with HPLC-MS/MS showed comparable results for acrylamide in ground water.

Indian Drugs 45(4), 301-306 (2008). HPTLC of diclofenac (extracted with 3N HCl and chloroform from single and multi-component diclofenac gel formulations) on silica gel with toluene - ethyl acetate - acetic acid 600:400:2. The hRf value of diclofenac was 39, of salicylic acid 29, and of methyl salicylate 83. Quantitative determination by absorbance measurement at 283 nm. Linearity was between 200 and 600 ng/spot via peak area. In single component gel, recovery was 100.4 % whereas in multi-component gel it was 99.5 %. The method was found to be accurate and suitable for analysis in single and multi-component gel formulations.

Asian J. Chem. 20(4), 2531-2538 (2008). TLC of melatonin on silica gel with toluene - ethyl acetate - formic acid 10:9:1. Quantitative determination by absorbance measurement at 290 nm. The method was linear in the concentration range of 100 to 600 ng/spot. The recovery of the drug from tablets (by standard addition method) was 99.7 %. Statistical analysis proves that the method is repeatable, selective and accurate for the estimation of the drug. Forced degradation studies showed the effect of variations in pH, UV light and high temperature on the stability of melatonin. As the proposed method could effectively separate the drug from its degradation products, it can be employed as a stability indicating method.

J. AOAC Int. 91, 1331-1338 (2008). HPTLC of ciprofloxacin (1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(piperazin-1-yl)quinoline-3-carboxylic acid hydrochloride) and degradation products (7-[(2-aminoethyl)amino]-1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-quinoline-3-carboxylic acid and 7-amino-1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-quinoline-3-carboxylic acid) on silica gel with chloroform - methanol - 25 % ammonia 43:43:14. Detection with 0.3 % methanolic ninhydrin solution and dimethylaminobenzaldehyde and quantitative determination by absorbance measurement at 277 nm.

Indian Drugs 44(12), 937-944 (2007). HPTLC of the methanolic extract of Sesamum indicum root (Pedaliaceae) and its ethyl acetate fraction on silica gel with ethyl acetate - n-hexane 1:9. Absorbance measurement at 549 nm. The red zone was isolated by preparative TLC and identified by IR to be a 1,4-naphthoquinone derivative.

60th Indian Phamaceutical Congress PA-132, (2008). HPTLC of lamivudine and zidovudine on silica gel with acetone - methanol - toluene 2:1:2. Quantitative determination by absorbance measurement at 273 nm. The method was suitable for routine quality control of both drugs in combined dosage form.