J. Liq. Chromatogr. Relat. Technol. 40, 22-286 (2017). HPTLC of clarithromycin (1), azithromycin (2), and amodiaquine (3) and artesunate (4) on silica gel with methanol – ethyl acetate – concentrated ammonium hydroxide 40:10:1 for (1) and (2) and acetone – water – concentrated ammonium hydroxide 40:7:2 for (3) and (4). Detection by heating for 15 and 30 min, respectively, for (1) and (2) at 160 °C to activate fluorescence quenching. Naturally fluorescence quenching zones of (3) were scanned at 254 nm and (4) after heating the plate for 5 min at 180 °C to activate fluorescence quenching.

J. Chromatogr. Sci. 54 (4), 609-617 (2016). HPTLC of sulbutiamine (SUL) in the presence of different degradation products after subjecting the drug to stress conditions (according to ICH: neutral, alkaline and acidic hydrolysis, oxidation, photodegradation and dry heat degradation), on silica gel aluminum foil with acetone – methylene chloride – ammonia buffer (pH 8.5±0.2) 14:6:1. Densitometric evaluation at 254 nm. The calibration curve was between 0.4–5.0 µg/zone with good correlation coefficients. The LOD and LOQ were 110 and 330 ng/zone, respectively. Structure elucidation of the resulting degradation products by ESI-MS/MS. The results showed that the drug was completely degraded with 0.1 N NaOH, 1 N HCl and 30 % hydrogen peroxide, while it was partially degraded by 0.1 N HCl, 3 % hydrogen peroxide and UV light. The hRf of SUL was 46 and the zone was completely separated from all obtained degradation products.

J. Ethnopharmacol. 213, 230-255 (2018). Review of the botany, ethnopharmacology, phytochemistry, biological activities, nutritional value, possible molecular mechanisms, safety and clinical applications of Morinda officinalis with a special focus on its bioactivities, including the application of HPTLC for the analysis of oligosaccharides from different habitats.

Food Control 86, 214-223 (2017). HPTLC of epicatechin (1) and proanthocyanidins PAC-A2 (2) and PAC-B2 (3) in cranberry juice on silica gel with dichloromethane – ethyl acetate – formic acid 3:5:1. Detection by spraying with anisaldehyde sulfuric acid reagent, followed by heating at 100 °C for 2 min. Quantitative determination by absorbance measurement at 200 nm. Intermediate precision was below 2 % (n=9). Recovery was between 99.9 and 100.1 % for (1) to (3).

CBS 117, 5-7 (2016). HPTLC of amylase-produced dextrins and standards glucose, maltose, maltotriose, maltotetrose, maltopentose, maltohexose, and maltoheptose on silica gel with acetonitrile – acetone – water 3:3:2 with chamber saturation to a migration distance of 60 mm. Detection by immersion into aniline-diphenylamine-phosphoric acid reagent (2 g diphenylamine and 2 mL aniline in 80 mL methanol in 10 mL 85 % phosphoric acid, made up to 100 mL with methanol) followed by heating at 120 °C for 5 min. Evaluation under white light. Quantitative determination by absorbance measurement at 500 nm. Evaluation in the polynomial working range of 40-210 ng/band.

J. Chromatogr. Sci. 55 (10), 1059-1065 (2017). Presentation of a new high-throughput method for the simultaneous analysis of isoflavones and soyasaponins in soy (Glycine max L.) products by HPTLC on silica gel with ethyl acetate – methanol – water – acetic acid 100:20:16:1. Detection by treatment with anisaldehyde sulfuric acid reagent. Quantitative determination by densitometric multi-wavelength scanning at UV 265 nm for genistin, daidzin and glycitin and at 650 nm for soyasaponins I and III. The correlation coefficient of the linear calibration curve was >0.994. Intra-day precision (%RSD) of substances in matrix was between 0.7-0.9 %, inter-day precision (%RSD) was between 1.2-1.8 %). The method was suitable for the determination of the studied analytes in soy-based infant formula and soybean products.

J. Sep. Sci. 1-9 (2017). HPTLC of canagliflozin (1) and its degradation product (2) on silica gel with acetone – ethanol 4:1. Quantitative determination by absorbance measurement at 290 nm. The hRF values for (1) and (2) were 64 and 81, respectively. Linearity was between 0.4 and 3.6 μg/zone for (1) and 0.2 and 3.2 μg/zone for (2). LOD and LOQ were 70 and 200 ng/zone for (2). The intermediate/interday/intra-day precisions were below 2 %. Average recovery was 100.7 % for (1) and 99.4 % for (2).

J. Liq. Chromatogr. Relat. Technol. 41, 73-82 (2018). Review of the applications of TLC for the analysis of both synthetic and natural sunscreens. The review contained a detailed description of techniques, materials and instruments of selected applications, including laboratory experiments for students, lipophilicity measurements, TLC-bioactivity detection, TLC-MS, separation and characterization of sunscreens, densitometric analysis of formulations, preparative layer chromatography, thin-layer radiochromatography, TLC of decomposition products and study of blood-barrier permeability. Future prospects were also discussed, including TLC–MS for sunscreen analysis.