J. Food Comp. Anal. 49, 94-101 (2016). HPTLC of chlorogenic acid (1), 3,4-dicaffeoylquinic acid (2), 4,5-dicaffeoylquinic acid (3), and 3,5-dicaffeoylquinic acid (4) in 295 varieties and breeding lines of sweet potatoes on silica gel with ethyl acetate – methanol – acetic acid – formic acid – water 27:2:2:2:2. Quantitative determination by absorbance measurement at 330 nm. The hRF values for (1) to (4) were 55, 85, 87 and 95, respectively. Intermediate precisions were below 3.4 %.

Food Chem. 210, 99-103 (2016). HPTLC of jasmonic acid in gamma-irradiated cabbage on silica gel with isopropanol – ammonia – water 10:1:1. Quantitative determination by absorbance measurement at 295 nm. The hRF values for jasmonic acid was 63. Linearity was in the range of 20-80 µg/zone. The LOD and LOQ were 6 µg/zone and 90 µg/zone, respectively. Recoveries were in the range 95.4-106.3 %.

CBS 116, 2-4 (2016). HPTLC of steroid alkaloid saponins from potato sprouts on silica gel with chloroform – methanol – water 60:40:9 with chamber saturation without filter paper over a migration distance of 70 mm. Detection by dipping half of the plate in anisaldehyde-sulfuric acid reagent, drying for 10 min and heating at 70 °C for 5 min. The other half of the plate was used for the blood-gelatin test: dipping in 0.5 % polyisobutylmethacrylate in n-hexane – chloroform 20:1 for 10 s, coating with 40 mL blood-gelatin (2 % gelatin in PBS buffer pH 7.4 with 3 % human blood preservation), storage for 12-48 h at 4 °C. Evaluation under UV 366 nm and white light. Semiquantitative evaluation of the hemolytic activity using the substance digitonin from potato sprouts. The hemolytic activity of α-chaconine was comparable to the effect of 16-20 µg digitonin.

J. Liq. Chromatogr. Relat. Technol. 39, 277-280 (2016). HPTLC of azithromycin (1), imipramine HCl (2), and sulfadoxine + pyrimethamine (3/4) on silica gel with methanol – ethyl acetate – concentrated ammonia 40:10:1 for (1), methanol – ammonia 96:1 for (2) and ethyl acetate – methanol 5:1 for (3/4). Quantitative determination by absorbance measurement at 254 nm. The hRF values were 54 for (1), 40 for (2), 67 for (3) and 45 for (4). Precisions for recovery were within 5 % and RSDs for triplicate assays and validation analyses at 50, 100, and 150 % spike levels were within 3 %._x000D_

J. Planar Chromatogr. 29, 184-189 (2016). HPTLC of (±)-etodolac mixed with L-Trp 1:1 as chiral inducing reagent on silica gel with acetonitrile – dichloromethane – methanol 3:1:2. Detection by using iodine vapor. The hRF values for (-)- and (+)-etodolac were 33 and 62, respectively.

J. Planar Chromatogr. 29, 423-428 (2016). HPTLC of lupeol (1) and ursolic acid (2) in the leaves of Bauhinia purpurea L., Bauhinia variegata L., Bauhinia acuminata L., and Bauhinia tomentosa L. on silica gel with toluene – ethyl acetate – formic acid 80:20:1. Detection by dipping into anisaldehyde – sulfuric acid reagent followed by heating at 40 °C for 3 min. Quantitative determination by absorbance measurement at 550 nm for (1) and 522 nm for (2). The hRF values for (1) and (2) were 46 and 68, respectively. Linearity was between 100 and 600 ng/zone for (1) and (2). The intermediate precisions were below 1.5 % (n=3). The LODs and LOQs were 40 and 100 ng/zone for (1) and 35 and 100 ng for (2). Recoveries were between 97.1 and 100.0 % for (1) and 98.0 and 99.2 % for (2).

ssp. extracts based on two fast and efficient chromatographic methods (high-performance thin-layer chromatography or high-performance liquid chromatography) for the quantification of total saponins. J. Planar Chromatogr. 29, 410-416 (2016). HPTLC of virgaureasaponins in the aerial parts and rhizomes of S. virgaurea alpestris and S. virgaurea virgaurea on silica gel with chloroform – acetic acid – methanol – water 15:8:3:2. Detection by dipping into a solution of anisaldehyde – sulfuric acid reagent, followed by heating at 105 °C during 5 min. Quantitative determination by absorbance measurement at 366 nm. The hRF values for virgaureasaponins were 39, 44, 49, 57 and 60. Linearity was between 10 and 30 μg/zone. The intermediate precisions were below 2 % (n=3). The LOD and LOQ were 2 and 10 μg/zone, respectively. Average recovery was 97.0 %.

J. Planar Chromatogr. 29, 388-390 (2016). HPTLC of dimethoate from post mortem viscera on silica gel with hexane - acetone 4:1. Detection by spraying with freshly prepared sodium nitroprusside solution (1 % sodium nitroprusside in 2 N sodium hydroxide solution prepared with especially sulfide-free distilled water). The hRF value for dimethoate was 88.