Indian Drugs 44(12), 937-944 (2007). HPTLC of the methanolic extract of Sesamum indicum root (Pedaliaceae) and its ethyl acetate fraction on silica gel with ethyl acetate - n-hexane 1:9. Absorbance measurement at 549 nm. The red zone was isolated by preparative TLC and identified by IR to be a 1,4-naphthoquinone derivative.

60th Indian Phamaceutical Congress PA-132, (2008). HPTLC of lamivudine and zidovudine on silica gel with acetone - methanol - toluene 2:1:2. Quantitative determination by absorbance measurement at 273 nm. The method was suitable for routine quality control of both drugs in combined dosage form.

60th Indian Pharmaceutical Congress PA-195, (2008). HPTLC of trandolapril on silica gel with toluene - ethyl acetate - methanol - formic acid 5:16:2:1. The hRf value of trandolapril was 51. Trandolapril was subjected to different stress conditions like acidic and alkaline hydrolysis, oxidation, dry heat, wet heat, neutral condition and photodegradation. The degradation products were well resolved from the pure drug. Quantitative determination by absorbance measurement at 220 nm. Linearity was between 300-1800 ng/spot. The method was suitable for routine analysis of the drug in bulk and pharmaceutical dosage form.

Science & Culture Jan.-Feb., 22-30 (2008). Isolation and identification of some unique chemical compounds is reported using chemical, chromatographic and spectroscopic methods such as GC-MS, HPTLC and HPLC. The presence of bio-organic molecules such as oxygenated dibenzo-a-pyrones (DBPs), their amino acyl conjugates (DCPs) and polyphenyl benzoquinones (PBQs) was observed in all the four samples of meteorites. HPTLC on silica gel with 1) n-butanol - acetone - acetic acid - water 7:7:2:4 for amino acids, detection with ninhydrine reagent and absorbance measurement at 610 nm; 2) n-butanol - acetic acid - water 3:1:2 for sugars, detection with p-anisidine reagent and absorbance measurement at 380 nm; 3) chloroform - methanol 9:1 for DBPs and absorbance measurement at 240 nm and 360 nm.

obtained by use of different techniques. J. Planar Chromatogr. 22, 25-28 (2009). HPTLC of flavonoids (rosmarinic acid and luteoline) on silica gel with ethyl acetate - formic acid - water 136:5:6, ethyl acetate - methanol - water 77:13:10, ethyl acetate - diethyl ether 4:1, n-hexane - ethyl acetate - formic acid 60:40:3, chloroform - methanol - formic acid 882:60:47, and chloroform - acetone - formic acid 19:4:2. The mobile phase chosen for quantification was chloroform - ethyl acetate - formic acid 60:40:3. Detection by spraying with natural products reagent, followed by polyethylene glycol 400 reagent. Quantitative determination by densitometry at 328 nm (rosmarinic acid) and 349 nm (luteoline).

J. Planar Chromatogr. 21, 299-304 (2008). HPTLC of 21 amino acids on silica gel impregnated with 5 % Ag ion with borate phosphate buffer pH 2.3. After drying at 50 °C detection by spraying with ninhydrin. With this system separation of alanine, phenylalanine and tryptophane could be achieved, as well as separation of alanine, tyrosine and tryptophan. Separation of these amino acids was neither achieved on conventional silical gel (without Ag impregnation) nor by addtion of buffered 5 % silver nitrate solution (pH 2.3) to the mobile phase.

Ind. J. Pharm. Sci. 70(5), 644-647 (2008). HPTLC of meloxicam on silica gel with ethyl acetate - cyclohexane - glacial acetic acid 325:175:1 with chamber saturation for 45 min. Quantitative determination by absorbance measurement at 353 nm. The method was linear in the range of 100-500 ng/zone, recovery was 100.3 %. The method was evaluated for stability (acid, base, thermal, oxidative, photodegradation) and degradation products were well separated from the main drug.

Abstract No. 0983, IHCB (2009). HPTLC of mahanimbine (a carbazole alkaloid isolated from Murraya koenigi) on silica gel with petroleum ether - chloroform 13:7. The hRf value of mahanimbine was 60. Quantitative determination by absorbance measurement at 254 nm. The method was linear in the concentration range of 50-250 ng/spot. Enzyme inhibition activity of methanol, petroleum ether, and chloroform exctracts of the plant and of mahanimbine was evaluated using galantamine as standard inhibitor of the enzyme.