A new approach in forensic analysis. J. Planar Chromatogr. 24, 108-112 (2011). HPTLC of ditalimfos, edifenfos, and tolclofos-methyl on silica gel, prewashed with methanol, with n-hexane - acetone 9:1 in a twin-trough chamber with saturation. Quantitative determination by densitometry in absorbance mode at 254 nm, the wavelength of maximum absorption for all three fungicides. The hRf values of all three organophosphorus fungicides increased with increasing contents of acetone in the mobile phase.

J. Ethnopharmacol. 137, 341-344 (2011). HPTLC of quercetin (1) and kaempferol (2) in the leaves of Argyreia speciosa on silica gel with toluene - ethyl acetate 93:7. Quantitative determination by absorbance measurement at 254 nm. The hRf values of (1) and (2) were 32 and 75, respectively and their amount was found to be 0.6 % and 0.2 %, respectively.

Journal of Pharmacy Research 4(5), 1353-1355 (2011). HPTLC of methanolic extracts of Aegle marmelos fruit pulp on silica gel with toluene - ethyl acetate - glacial acetic acid 70:30:1. Densitometric quantification of marmelosin at 310 nm. The method was linear in the range of 50-350 ng/band. The method was used to determine the marmelosin content of pulp, unripe pulp, seeds, leaves, rind, outer stain and inner stain.

J. AOAC Int. 95, 992-1009 (2012). In his profound 22nd planar chromatography biennial review, the author presented the most important advances published between November 1, 2009 and November 1, 2011. The excellent review covered different aspects such as history, student experiments, books, reviews, theory and fundamental studies, chromatographic systems, apparatus and techniques, as well as quantitative analysis with a broad range of applications such as analysis of pharmaceuticals, herbal medicines, and dietary supplements, biological and clinical samples, foods and beverages, environmental samples and chemicals.

J. Planar Chromatogr. 25, 314-319 (2012). HPTLC of 4-odemethylpodophyllotoxin (1), podophyllotoxin (2), kaempferol (3), podophyllotoxone (4), and deoxypodophyllotoxin (5) in the rhizomes of Podophyllum hexandrum on silica gel with toluene - ethyl acetate 2:1 + 1 drop glacial acetic acid. Quantitative determination by absorbance measurement at 254 nm. Linearity was in the range of 1-8 µg/band for (1), (2) and (4) and 2-10 µg/band for (3) and (5). The intermediate/inter-day/intra-day precision was below 2 %. Average recovery for all (1) to (4) were between 96.4 and 101.8 %.

J. AOAC Int. 95, 1371-1377 (2012). HPTLC of organophosphate and carbamate pesticides such as chlorpyrifos (1), paraoxon (2), parathion (3) and pirimicarb (4) in fresh fruits and vegetables on silica gel in an automatic development chamber with methanol – dichloromethane 1:9 and n-hexane – ethyl acetate - dichlormethane 13:4:3. Matrix interferences were visualized at 366 nm after dipping in primuline reagent (0.5 g/L in acetone – water 4:1). Detection by immersion in a solution of rabbit liver esterase or cutinase, followed by horizontal incubation for 30 min at 37 °C. The enzymatic reaction was stopped by heating the plate at 50 °C for 5-7 min. Staining was performed with a mixture of 60 mL Fast Blue Salt B (2.5 g/L in water) and 30 mL alpha-naphythyl acetate (2.5 g/L in ethanol). Recoveries were in the ranges of 86-109, 95-129, 96-114 and 90-111 % for pesticides (1) to (4), respectively. Mean %RSD was 8.5 for all samples.

J. Planar Chromatogr. 25, 420-425 (2012). HPTLC of juglone (1), quercetin (2), myricetin (3), rutin (4), caffeic acid (5), and gallic acid (6) in the stem bark of Juglans regia L. on RP-18 with methanol - water - formic acid - acetic acid 61:58:3:3. Quantitative determination by absorbance measurement at 254 nm. The hRf values of compounds (1) to (6) were 16, 20, 31, 42, 55 and 78, respectively. Linearity was in the range of 13-800 ng/zone for (1) and (5), 100-1000 ng/zone for (2) and (6) and 50-800 ng/zone for (3) and (4). Limits of detection and quantification were 4 and 13 ng/zone for (1) and (5), 30 and 100 ng/zone for (2), 15 and 50 ng/zone for (3), (4) and (5), respectively. The intra-day and inter-day precisions for compounds (1) to (6) were in the range of 0.6-1.3 % and 0.5-1.9 %, respectively. Average recovery was between 98.6 and 101.1 %.

J. Liq. Chromatogr. Relat. Technol. 35, 1459-1480 (2012). HPTLC of itraconazole on silica gel with toluene - ethyl acetate 1:5 + 1 drop ammonia. Quantitative determination by absorbance measurement at 266 nm. The hRf value of itraconazole was 77 and selectivity regarding matrix was given. Linearity was in the range of 50-2000 ng/zone. The intra-day and inter-day accuracy were in the range of 81.9-97.8 and 89.2-97.1 %, respectively. The limits of detection and quantification were 14 and 43 ng/zone, respectively. Recovery (by standard addition) was between 99.6 and 100.2 %.