Innov. Food Sci. Emerg. Technol. 32, 127-135 (2015). HPTLC of prebiotic oligosaccharides produced in the orange juice on silica gel with acetonitrile - ethyl acetate - 1-propanol - water 17:4:10:18. Detection by spraying with 0.3 % 1-naphthyl ethylenediamine dihydrochloride in methanol with sulfuric acid 3 %, followed by heating at 120 °C for 10 min. The method was applied to determine the degree of polymerization. _x000D_

with the reference of protostemonine. J. Planar Chromatogr. 28, 448-451 (2015). HPTLC of protostemonine in Stemona sessilifolia on silica gel with ammonium hydroxide solution (25 %, v/v) – acetone – ethyl acetate – cyclohexane 1:4:4:6. Detection by spraying with diluted bismuth potassium iodide solution followed by drying at room temperature and spraying with 5 % sodium nitrite in 70 % ethanol solution. The hRF value for protostemonine was 30. The intermediate precision was below 2 % (n=5).

J. Ethnopharmacol. 181, 236-251 (2016). Analysis of the botanical description, geographical location, traditional and medicinal uses, phytochemistry and pharmacological investigation, toxicological aspects, patent information and clinical studies of Symplocos racemosa. The review described methods for the determination of loturine in the bark extracts of Symplocos racemosa on silica gel with chloroform – acetonitrile – triethylamine 7:5:2, quantitative determination by absorbance measurement at 280 nm.

J. Planar Chromatogr. 29, 287-298 (2016). HPTLC of 34 structurally diverse drugs on RP-18 with acetonitrile – pH 7.4 phosphate buffered saline 7:3. Detection under UV 254 nm. Molecular descriptors were investigated to assign the compounds to two clusters: central nervous system active (CNS+) or inactive (CNS-) describing the ability of the compounds to penetrate the brain.

J. Food Comp. Anal. 49, 94-101 (2016). HPTLC of chlorogenic acid (1), 3,4-dicaffeoylquinic acid (2), 4,5-dicaffeoylquinic acid (3), and 3,5-dicaffeoylquinic acid (4) in 295 varieties and breeding lines of sweet potatoes on silica gel with ethyl acetate – methanol – acetic acid – formic acid – water 27:2:2:2:2. Quantitative determination by absorbance measurement at 330 nm. The hRF values for (1) to (4) were 55, 85, 87 and 95, respectively. Intermediate precisions were below 3.4 %.

Food Chem. 210, 99-103 (2016). HPTLC of jasmonic acid in gamma-irradiated cabbage on silica gel with isopropanol – ammonia – water 10:1:1. Quantitative determination by absorbance measurement at 295 nm. The hRF values for jasmonic acid was 63. Linearity was in the range of 20-80 µg/zone. The LOD and LOQ were 6 µg/zone and 90 µg/zone, respectively. Recoveries were in the range 95.4-106.3 %.

CBS 116, 2-4 (2016). HPTLC of steroid alkaloid saponins from potato sprouts on silica gel with chloroform – methanol – water 60:40:9 with chamber saturation without filter paper over a migration distance of 70 mm. Detection by dipping half of the plate in anisaldehyde-sulfuric acid reagent, drying for 10 min and heating at 70 °C for 5 min. The other half of the plate was used for the blood-gelatin test: dipping in 0.5 % polyisobutylmethacrylate in n-hexane – chloroform 20:1 for 10 s, coating with 40 mL blood-gelatin (2 % gelatin in PBS buffer pH 7.4 with 3 % human blood preservation), storage for 12-48 h at 4 °C. Evaluation under UV 366 nm and white light. Semiquantitative evaluation of the hemolytic activity using the substance digitonin from potato sprouts. The hemolytic activity of α-chaconine was comparable to the effect of 16-20 µg digitonin.

J. Liq. Chromatogr. Relat. Technol. 39, 277-280 (2016). HPTLC of azithromycin (1), imipramine HCl (2), and sulfadoxine + pyrimethamine (3/4) on silica gel with methanol – ethyl acetate – concentrated ammonia 40:10:1 for (1), methanol – ammonia 96:1 for (2) and ethyl acetate – methanol 5:1 for (3/4). Quantitative determination by absorbance measurement at 254 nm. The hRF values were 54 for (1), 40 for (2), 67 for (3) and 45 for (4). Precisions for recovery were within 5 % and RSDs for triplicate assays and validation analyses at 50, 100, and 150 % spike levels were within 3 %._x000D_