Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      125 043
      Comparative study of HPLC-DAD and HPTLC for the simultaneous determination of a new multitarget antidiabetic ternary mixture in combined tablets
      O. EL-SHOUBASHY*, Y BELTAGY, A. ISSA, D. EL-KAFRAWY (*Pharmaceutical Chemistry Department, Faculty of Pharmacy, University of Alexandria, Elmessalah, Alexandria 21521, Egypt, dinaelkafrawy@yahoo.com)

      J. Planar Chromatogr. 33, 59-70 (2020). HPTLC of metformin (1), saxagliptin (2) and dapagliflozin (3) on silica gel with chloroform - methanol - water - acetic acid 740:260:50:1. Quantitative determination by absorbance measurement at 224 nm. The hRF values for (1) to (3) were 14, 50 and 66, respectively. Linearity was between 30 and 350 µg/mL for (1), 140 and 1500 µg/mL for (2) and 50 and 600 µg/mL for (3).  Intermediate precisions were below 2 % (n=6). LOD and LOQ were 7 and 23 µg/mL for (1), 39 and 130 µg/mL for (2) and 14 and 47 µg/mL for (3), respectively. Recovery rate was between 98.9 and 100.5 % for (1), 99.2 and 100.5 % for (2) and 99.2 and 100.7 % for (3). Comparable results were obtained when compared with a HPLC method.

      Classification: 32a
      125 002
      High-performance thin-layer chromatography/bioautography and liquid chromatography-mass spectrometry hyphenated with chemometrics for the quality assessment of Morus alba samples
      P. M. RISTIVOJEVIĆ, A. TAHIR, F. MALFENT, D. MILOJKOVIĆ OPSENICA, JUDITH M. ROLLINGER* (*Fac. of Life Sci., Dep. of Pharmacognosy, Univ. of Vienna, judith.rollinger@univie.ac.at)

      J. Chromatogr. A 1594, 190-198 (2019). Quality assessment of herbal drugs, e.g. Morus alba L. root bark (sāng bái pí; SBP), using a HPTLC/bioautography method. Identification of the most bioactive constituents with radical scavenging (DPPH radical assay) and antimicrobial activities (Bacillus subtilis, Escherichia coli) of 18 different M. alba samples, supported by UPLC–MS/MS analysis. Higher both radical scavenging and antimicrobial activities were found for plant materials collected from Serbia (11 samples) compared to samples provided from China (7 samples). Confirmation of geographically different samples and recognization of their main markers responsible for differences between Serbian and Chinese samples by principal component analysis (PCA). The developed procedure allowed not only for a fast chemical profiling of the investigated samples and their unambiguous identification, but also for the disclosure of major and minor bioactive constituents present in SBP.

      Classification: 32
      125 024
      Development and validation of high-performance thin-layer chromatographic method for quantification of berberine in rhizomes of Coptis teeta Wall, an endangered species collected from Arunachal Pradesh, India
      A. KUMAR GOSWAMI, N. GOGOI, A. SHAKYA, H. KUMAR SHARMA* (*Dep. of Pharm. Sci., Dibrugarh Univ., Dibrugarh, Assam, India, Email: hemantasharma123@yahoo.co.in)

      J. Chromatogr. Sci. 57 (5), 411-417 (2019). The high polarity of the has made it difficult to quantify the alkaloids. This study was designed to develop and validate a thin-layer chromatography (TLC) densitometric-based method using high-performance thin-layer chromatography for quantification of berberine. HPTLC of the protoberberine alkaloids in Coptis teeta rhizome on silica gel aluminium foil with butanol - ethyl acetate - formic acid - water 3:5:1:1. Quantitative determination by absorbance measurement at 351 nm. The hRf of berberine was 70. The linearity obtained graphically with a correlation coefficient of 0.997 was in the concentration range of 90-210 ng/band.LOD and LOQ were 30 and 70 ng/band, respectively. The berberine concentration in the methanol extract of C. teeta was 30.9 ± 0.6 mg in 100 mg of the crude drug. The method developed here in can be implemented in the analysis and routine quality control of herbal materials and formulations containing C. teeta and berberine.

      Classification: 32
      125 023
      High-performance thin-layer chromatography method for simultaneous determination of antipsychotic and medicinally important five β-carboline alkaloids
      N. SURYAKANT KADAM, A. ASHOKRAO NAIK, P. JIGNESH DOSHI, T. DAYARAM NIKAM* (*Department of Botany, Savitribai Phule Pune University, Pune, MH, India, tdnikam@unipune.ac.in, tdnikam37@gmail.com)

      J. Chromatogr. Sci. 57 (4), 312-322 (2019). Development of a method for simultaneous analysis of five antipsychotic and medicinally important β-carboline alkaloids (βCAs), namely, harmalol, harmaline, harmine, harmane and norharmane, by HPTLC on silica gel with chloroform - methanol - glacial acetic acid 39:11:1. Quantification by densitometry in fluorescence mode at 366 nm. The linearity range of standard βCAs was 25-250 ng/band, with r2 between 0.97 and 0.99. The recovery was between 83.9 and 112.4 %, repeatability of the application 0.6-2.4 %, repeatability of measurement 1.9-3.1 % and intermediate precision 0.6-11.2 %). LOD and LOQ were 4.9-6.6 and 16.5-21.9 ng/band, respectively. The method proved to be simple, cost-effective, precise, sensitive and specific for the determination of βCAs in the herbs Fagonia schweinfurthii, Peganum harmala and Tribulus terrestris, and was useful in forensic and industrial analysis and fingerprinting of various βCAs containing herbs and drug formulations.

      Classification: 32
      125 022
      HPTLC-densitometric method for determination of ascorbic acid, paracetamol and guaifenesin in presence of their toxic impurities
      N.S. ABDELWAHAB, E.A. ABDELALEEM*, M.M. ABDELRAHMAN (*Pharm. Anal. Chem. Dep., Fac. of Pharmacy, Beni-Suef Univ., Alshaheed Shehata Ahmad Hegazy St., Beni-Suef, Egypt, eglal_bardisi@yahoo.com)

      J. of Chromatogr. Sci. 57 (2), 149-155 (2019). Description of a selective stability indicating method for chromatographic separation of ascorbic acid (ASC), paracetamol (PAR) and guaifenesin (GUF) in presence of paracetamol toxic impurity [4-aminophenol (4-AP)] and guaifenesin related substance [impurity, (guaiacol) (GUC)] by HPTLC on silica gel with chloroform - acetone - trifluoroacetic acid 65:35:3, detection under UV 254 nm. Quantification by densitometry. The hRf values were 5 (ASC), 12 (4-AP), 24 (GUF), 41 (PAR), and 56 (GUC). The linearity was in the range of 0.4-2.4, 0.4-2.8 and 4-15 μg/band for ASC, PAR and GUF, respectively. Statistical comparison of the obtained results with those achieved by HPLC showed no significant difference. The HPTLC method proved to have advantages over HPLC, it was more sensitive and selective and permits its application in quality control of the drugs.

      Classification: 32
      125 017
      Determination of quinalphos in human whole blood samples by high-performance thin-layer chromatography for forensic application
      P. U. SANGANALMATH, P. M. NAGARAJU, K. SREERAMULU* (*Dep. of Biochem., Gulbarga Univ., Gulbarga 585106, India, ksramu@rediffmail.com)

      J. Chromatogr. A 1594, 181-189 (2019). Development of a simple and rapid procedure for the determination of quinalphos in human whole blood by HPTLC on silica gel with n-hexane – acetone 9:1 after extraction from spiked blood samples with the optimum solvent, diethyl ether, at pH 3 (average recovery = 93.6%), detection and quantification by densitometry at 325 nm in absorbance mode. Validation by examination of the effect of different organic solvents and pH on the extraction yield of quinalphos, and by investigation of the interference of other organophosphorus pesticides of forensic relevance (not observed). The linearity was in the range of 1 to 100 μg/mL with r2 = 0.9981, the sensitivity (LLOQ) at 1 μg/mL. The within-day precision and between-day precision ranged from 0.2 to 1.0%, and 0.1 to 0.8%, respectively, with an overall average recovery of 91.1% at three concentrations 1, 10, and 50 μg/mL. For different storage conditions for the samples no significant decrease in the concentration of quinalphos was observed. Application of developed procedure in three fatal cases of poisoning.

      Classification: 29b
      125 013
      Comprehensive HPTLC fingerprinting for quality control of an herbal drug – the case of Angelica gigas root
      Débora Arruda FROMMENWILER*, J. KIM, C. YOOK, T. T. T. TRAN, S. CAÑIGUERAL, E. REICH (*CAMAG Laboratory, Muttenz, Switzerland; debora.frommenwiler@camag.com)

      Planta Medica 84(6/7), 465-474 (2018). The new concept “Comprehensive HPTLC Fingerprinting” was applied to define specifications for the identification and purity assessment of Angelica gigas roots, and for the quantification of its markers: the coumarins decursin and decursinol angelate. Methanolic root extracts of A. gigas (10 reference materials, 24 commercial samples), of 26 other Apiaceae species (including 10 Angelica, 9 Ligusticum, 2 Notopterygium, 4 Peucedanum, and Levisticum officinale) and of mixtures, were developed with toluene - ethyl acetate - acetic acid 90:10:1 on HPTLC silica gel (at 33% relative humidity, chamber pre-saturated for 20 min with filter paper and developing solvent) and dried for 5 min. Detection under white and UV lights before and after derivatization by dipping into 10% sulfuric acid in methanol and then heating 3 min at 100°C. Quantitative evaluation by densitometry in fluorescence mode at UV 313 nm, and luminance was also calculated from the image pixels. The study showed the presence in A. gigas of nodakenin, decursinol, 7-demethylsuberosin, imperatorin, osthole, and isoimperatorin at hRF 0, 4, 15, 33, 38 and 44 respectively. Z-ligustilide (hRF 59) was absent from A. gigas, allowing 1) to distinguish it from several other Apiaceae species; 2) to identify in mixtures with A. gigas two common adulterants (A. acutiloba, A. sinensis) even at 1% in the root powder. Minimal content of A. gigas fingerprint markers (decursin + decursinol acetate, co-eluting at hRF 27) was assessed as 3% (w/w) based on the quantified peaks from A. gigas reference materials.

      Classification: 2f, 8b, 32e
      124 059
      Simultaneous high-performance thin-layer chromatographic quantification of withaferin A and withanolide A in Solanum nigrum L. “Black Nightshade”
      R. PREET*, R. GUPTA, M. KAUR (*Department of Botany, Punjabi University, Patiala, India, ramanbrar247@gmail.com)

      J. Planar Chromatogr. 32, 339-342 (2019). HPTLC of withaferin A (1) and withanolide A (2) in Solanum nigrum on silica gel with toluene - ethyl acetate - formic acid - ethanol 60:30:1:6. Detection by spraying with p-anisaldehyde sulfuric acid (1 mL of p-anisaldehyde solution in 2 mL of concentrated sulfuric acid and 100 mL of acetic acid). Quantitative determination by absorbance measurement at 530 nm. The hRF values for (1) and (2) were 43 and 55, respectively. 

      Classification: 14
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