Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

      131 043
      The importance, prevalence and determination of vitamins B6 and B12 in food matrices: A review
      A. SANTOS*, S. KHEMIRI, S. SIMOES, C. PRISTA, I. SOUSA, A. RAYMUNDO (*Linking Landscape, Environment, Agriculture and Food (LEAF) Research Centre, Higher Institute of Agronomy of the University of Lisbon, Tapada da Ajuda, 1349-017 Lisbon, Portugal,

      Food Chem. 426, 136606 (2023). Review of different aspects of vitamins B6 and B12, including methods of determination and respective matrices, with emphasis on chromatographic techniques. Detailed information regarding vitamin B6 and B12 concentrations determined in selected food matrices, along with the respective TLC and HPTLC analytical methods was described.

      Keywords: HPTLC review
      Classification: 1b, 27
      129 068
      Thai mango and pineapple puree and juice analyzed by high-performance thin-layer chromatography hyphenated with effect-directed assays
      (*Institute of Nutritional Science, and Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Giessen, Germany;

      Molecules, 26 (24), 7683 (2021). Samples were ultrasound-assisted extracts of fruit puree and juice (pre-treated with sulfur dioxide or ascorbic acid) of Ananas comosus (Bromeliaceae) and Mangifera indica (Anacardiaceae). HPTLC on silica gel with toluene – ethyl acetate – methanol – formic acid 120:90:35:3. Detection under white light, UV 254 nm and 366 nm, before and after  derivatization by immersion (2 s, 3 cm/s) into anisaldehyde sulfuric acid reagent and  diphenylamine aniline reagent, followed by heating at 110 °C for 5 min. Effect-directed analysis using automated immersion: A) for free radical (DPPH•) scavengers; B) for enzymatic inhibition (acetyl-cholinesterase, tyrosinase); C) for activity against Gram-negative (Aliivibrio fischeri bioluminescence assay) or Gram-positive bacteria (Bacillus subtilis bioassay). Active compounds were far more present in puree than in juice extracts, and differences were also seen between cultivars. Ascorbic acid (hRF 37), used as additive for the mango puree, was active as antioxidant and as transiently disruptive for A. fischeri metabolism and bioluminescence.

      Classification: 27, 32e, 35b
      129 052
      Herbal standardization of formulation containing curcuminoids, piperine and ascorbic acid by dual detection mode densitometric analysis
      P. PATEL*, J. BHATT, F. SUREJA, M. DHORU, K. DETHOLIA (*Department of Pharmaceutical Quality Assurance, Smt. S.M. Shah Pharmacy College, Mahemdabad, Gujarat, India,

      J. Planar Chromatogr. 34, 513-520 (2021). HPTLC of curcumin (1), piperine (2) and ascorbic acid (3) in a combined marketed formulation on silica gel with chloroform - methanol - ether - glacial acetic acid 95:3:1:1. Quantitative determination by absorbance measurement at 254 nm for (3) and in fluorescence mode at 366 nm for (1) and (2). The hRF values for (1) to (3) were 65, 83 and 28, respectively. Linearity was between 620 and 3040 ng/zone for (1), 470 and 2380 ng/zone for (2) and 530 and 2670 ng/zone for (3). Interday and intra-day precisions were below 2 % (n=3). 

      Classification: 22, 27
      129 002
      Imaging high-performance thin-layer chromatography as powerful tool to visualize metabolite profiles of eight Bacillus candidates upon cultivation and growth behavior
      S. KRUSE, F. PIERRE, Gertrud E. MORLOCK* (*Institute of Nutritional Science, and Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Giessen, Germany;

      J Chromatogr A, 1640, 461929 (2021). Study of the impact of different strains, culture media and parameters (temperature, time, rotational speed, and glucide and amino-acid supply) on the metabolite profile of bacteria. Samples were cultivation broths of Bacillus subtilis, B. licheniformis, B. pumilus and B. amyloliquefaciens, as well as their respective supernatant liquid-liquid extracts (apolar solvents only or QuEChERS method with acetonitrile and MgSO4 – NaCl mixture 4:1). HPTLC on silica gel (normal phase and RP-18), either as bands (for small volumes of extracts) or as areas for supernatants and bigger volumes of extracts. Extract areas were focused with a three-step procedure (up to 20mm with acetone, and twice with methanol); unextracted supernatants were focused twice with methanol and once with tetrahydrofuran, but the application zone of the plate had to be cut before development, due to the high matrix load. Development with ethyl acetate – methanol – water at different ratios after activation of the plate surface with magnesium chloride (33% relative humidity), evaluation in white light and UV. Detection of antibacterial compounds with Aliivibrio fischeri bioassay. Derivatization with primuline (for lipophilic substances) and diphenylamine aniline sulfuric acid reagent (for saccharides). This method allowed a fast comparison: A) of the patterns of the different strains (presence /absence and intensity of detected or antibacterial bands); B) of cultivation parameters: the number of metabolites increased with time, rotational speed (oxygen level), and at 37°C (vs. 30°C), whereas a minimal medium allowed the detection of more metabolites, due to the lower matrix load; C) of the impact of the extraction parameters: choice of the solvents (QuEChERS method had no advantage here), solvent – supernatant ratio (1:3 showed richer patterns than 1:1); D) of the HPTLC parameters used (better separation and resolution with normal phase vs. RP18 layers).

      Classification: 3a, 10a, 11c, 27
      128 002
      Assessment of nutritional value and quantitative analysis of bioactive phytochemicals through targeted LC-MS/MS method in selected scented and pigmented rice varietals
      P. CHOUDHURY, K. DUTTA, A. SINGH, D. MALAKAR, M. PILLAI, N. TALUKDAR, S. SAMANTHA*, R. DEVI (*Institute of Advanced Study in Science and Technology, Paschim Boragaon, Guwahati, Assam 781035, India,

      J. Food Sci. 85, 1781-1792 (2020). HPTLC of scented (joha) rice and black rice variety on silica gel with chloroform - methanol 19:1 + 0.1 % formic acid and butanol - acetic acid - water 4:1:5. Detection by spraying with p-anisaldehyde and visualization under UV light at 200, 250 and 350 nm. The husk of the selected rice varieties contained the nonpolar metabolites whereas the seeds contain nonpolar as well as polar metabolites.

      Classification: 7, 27
      128 042
      Determination of the effect of germination on the folate content of the seeds of some legumes using HPTLC-mass spectrometry-multivariate image analysis
      S. SALLAM, E. SHAWKY*, S. EL SOHAFY (*Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Egypt,

      Food Chem. 362, 130206 (2021). HPTLC of 5-methyl tetrahydrofolate (1), tetrahydrofolate (2), pteroyl glutamate (3), 5-formyl tetrahydrofolate (4) and 10-formyl tetrahydrofolate (5) in legume seeds on silica gel with ethyl acetate - methanol - water - acetic acid 18:5:4:1. Detection under UV light at 254 and 366 nm. Quantitative determination by absorbance measurement at 366 nm. The hRF values for (1) to (5) were 69, 56, 43, 22 and 18, respectively. The intermediate precision was below 2 % (n=6). The LOD and LOQ were 10 and 29 ng/zone for (1), 19 and 57 ng/zone for (2), 9 and 28 ng/zone for (3), 10 and 29 ng/zone for (4) and 13 and 40 ng/zone for (5), respectively. Average recovery was 97.5 % for (1), 98.4 % for (2), 97.5 % for (3), 97.6 % for (4) and 97.8 % for (5).

      Classification: 27
      126 037
      Vitamins determination by TLC/HPTLC—a mini‑review
      Q. ULLAH*, A. MOHAMMAD (*Physical Sciences Section, School of Sciences, Maulana Azad National Urdu University, Gachibowli, Hyderabad, TS 500032, India,

      J. Planar Chromatogr. 33, 429-437 (2020). Review of the application of TLC and HPTLC for the analysis of vitamins covering the period of 2011-2019. The authors describe stationary and mobile phases for the TLC/HPTLC analysis of water and fat-soluble vitamins. The review highlightes that none of the papers reviewed showed the use of surfactants or ionic materials as impregnating agents or mobile phase modifers.

      Keywords: HPTLC review
      Classification: 27
      125 056
      Effect of superfine pulverization technology on the determination of vitamin C content in Fructus rosae laxae
      J. SUN (Sun Jing)*, A. ZHANG (Zhang Airong), N. WANG (Wang Ning), L. TIAN (Tian Li)* (*College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, Xinjiang, China,

      J. Planar Chromatogr. 33, 79-87 (2020). HPTLC of vitamin C in the dried fruit of Rosa laxa on silica gel with ethyl acetate - absolute ethanol - water 40:24:15. Quantitative determination by absorbance measurement at 273 nm. The hRF value for vitamin C was 61. Linearity was between 0.25 and 1.2 µg/zone. Intermediate precisions were below 2 % (n=6). Average recovery was 98.4 %.


      Classification: 27, 32e