Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
- Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
- Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
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Molecules, 26 (24), 7683 (2021). Samples were ultrasound-assisted extracts of fruit puree and juice (pre-treated with sulfur dioxide or ascorbic acid) of Ananas comosus (Bromeliaceae) and Mangifera indica (Anacardiaceae). HPTLC on silica gel with toluene – ethyl acetate – methanol – formic acid 120:90:35:3. Detection under white light, UV 254 nm and 366 nm, before and after derivatization by immersion (2 s, 3 cm/s) into anisaldehyde sulfuric acid reagent and diphenylamine aniline reagent, followed by heating at 110 °C for 5 min. Effect-directed analysis using automated immersion: A) for free radical (DPPH•) scavengers; B) for enzymatic inhibition (acetyl-cholinesterase, tyrosinase); C) for activity against Gram-negative (Aliivibrio fischeri bioluminescence assay) or Gram-positive bacteria (Bacillus subtilis bioassay). Active compounds were far more present in puree than in juice extracts, and differences were also seen between cultivars. Ascorbic acid (hRF 37), used as additive for the mango puree, was active as antioxidant and as transiently disruptive for A. fischeri metabolism and bioluminescence.
J. Planar Chromatogr. 34, 513-520 (2021). HPTLC of curcumin (1), piperine (2) and ascorbic acid (3) in a combined marketed formulation on silica gel with chloroform - methanol - ether - glacial acetic acid 95:3:1:1. Quantitative determination by absorbance measurement at 254 nm for (3) and in fluorescence mode at 366 nm for (1) and (2). The hRF values for (1) to (3) were 65, 83 and 28, respectively. Linearity was between 620 and 3040 ng/zone for (1), 470 and 2380 ng/zone for (2) and 530 and 2670 ng/zone for (3). Interday and intra-day precisions were below 2 % (n=3).
J Chromatogr A, 1640, 461929 (2021). Study of the impact of different strains, culture media and parameters (temperature, time, rotational speed, and glucide and amino-acid supply) on the metabolite profile of bacteria. Samples were cultivation broths of Bacillus subtilis, B. licheniformis, B. pumilus and B. amyloliquefaciens, as well as their respective supernatant liquid-liquid extracts (apolar solvents only or QuEChERS method with acetonitrile and MgSO4 – NaCl mixture 4:1). HPTLC on silica gel (normal phase and RP-18), either as bands (for small volumes of extracts) or as areas for supernatants and bigger volumes of extracts. Extract areas were focused with a three-step procedure (up to 20mm with acetone, and twice with methanol); unextracted supernatants were focused twice with methanol and once with tetrahydrofuran, but the application zone of the plate had to be cut before development, due to the high matrix load. Development with ethyl acetate – methanol – water at different ratios after activation of the plate surface with magnesium chloride (33% relative humidity), evaluation in white light and UV. Detection of antibacterial compounds with Aliivibrio fischeri bioassay. Derivatization with primuline (for lipophilic substances) and diphenylamine aniline sulfuric acid reagent (for saccharides). This method allowed a fast comparison: A) of the patterns of the different strains (presence /absence and intensity of detected or antibacterial bands); B) of cultivation parameters: the number of metabolites increased with time, rotational speed (oxygen level), and at 37°C (vs. 30°C), whereas a minimal medium allowed the detection of more metabolites, due to the lower matrix load; C) of the impact of the extraction parameters: choice of the solvents (QuEChERS method had no advantage here), solvent – supernatant ratio (1:3 showed richer patterns than 1:1); D) of the HPTLC parameters used (better separation and resolution with normal phase vs. RP18 layers).
J. Food Sci. 85, 1781-1792 (2020). HPTLC of scented (joha) rice and black rice variety on silica gel with chloroform - methanol 19:1 + 0.1 % formic acid and butanol - acetic acid - water 4:1:5. Detection by spraying with p-anisaldehyde and visualization under UV light at 200, 250 and 350 nm. The husk of the selected rice varieties contained the nonpolar metabolites whereas the seeds contain nonpolar as well as polar metabolites.
Food Chem. 362, 130206 (2021). HPTLC of 5-methyl tetrahydrofolate (1), tetrahydrofolate (2), pteroyl glutamate (3), 5-formyl tetrahydrofolate (4) and 10-formyl tetrahydrofolate (5) in legume seeds on silica gel with ethyl acetate - methanol - water - acetic acid 18:5:4:1. Detection under UV light at 254 and 366 nm. Quantitative determination by absorbance measurement at 366 nm. The hRF values for (1) to (5) were 69, 56, 43, 22 and 18, respectively. The intermediate precision was below 2 % (n=6). The LOD and LOQ were 10 and 29 ng/zone for (1), 19 and 57 ng/zone for (2), 9 and 28 ng/zone for (3), 10 and 29 ng/zone for (4) and 13 and 40 ng/zone for (5), respectively. Average recovery was 97.5 % for (1), 98.4 % for (2), 97.5 % for (3), 97.6 % for (4) and 97.8 % for (5).
J. Planar Chromatogr. 33, 429-437 (2020). Review of the application of TLC and HPTLC for the analysis of vitamins covering the period of 2011-2019. The authors describe stationary and mobile phases for the TLC/HPTLC analysis of water and fat-soluble vitamins. The review highlightes that none of the papers reviewed showed the use of surfactants or ionic materials as impregnating agents or mobile phase modifers.
J. Planar Chromatogr. 33, 79-87 (2020). HPTLC of vitamin C in the dried fruit of Rosa laxa on silica gel with ethyl acetate - absolute ethanol - water 40:24:15. Quantitative determination by absorbance measurement at 273 nm. The hRF value for vitamin C was 61. Linearity was between 0.25 and 1.2 µg/zone. Intermediate precisions were below 2 % (n=6). Average recovery was 98.4 %.
J. Planar Chromatogr. 20, 483-486 (2007). New method for determination of log P for selected tocopherols, which makes use of Rf, topological indices, and log P according to Rekker. HPTLC of alpha-, beta-, gamma-, and delta-tocopherols on RP-18 with ethanol or ethanol - water 19:1. Detection by spraying with a mixture of equal volumes of solutions of dipyridyl in methanol (0.5%) and iron(III) chloride in methanol (0.2%).