J. Chromatogr. 262, 460-463 (1983). Separation of the vitamin D3 metabolites by HPTLC. This method is rapid and, given the capacity to analyze several samples on a l0xl0 cm plate, can be a time-saving alternative to HPLC for routine analysis of partially purified samples. HPTLC of mono- and dihydroxylated metabolites of vitamin D on silica with a) dichloromethane - isopropanol 9:1, b) hexane - isopropanol 85:15, c) chloroform - ethyl acetate 5:5. Detection by UV 254 nm.

Chromatogr. Sci. 30, 129-200 (1985). A review with 216 references on chromatography (gas, liquid, paper and thin-layer) of the title compound. in biological materials.

Internat. J. Vit. Nutr. Res. 55, 139-147 (1986). TLC on aluminium oxide with petrol ether - ether 98:2. Quantification after elution by photometry at 450 nm.

J. Planar Chromatogr. 4, 416 (1991). TLC of a-tocopherol on silica with benzene - acetone 9:1. Quantification after elution by spectrometry.

J. Planar Chromatogr. 10, 447-452 (1997). TLC of organic acids (malic, citric, tartaric, ascorbic acid), carbohydrates (fructose, sucrose, galacturonic acid), fat-soluble vitamins (vitamin D1, D2, tocopherols), amino acids and anthocyanins on corn starch layers, rice starch layers, talc layers, and impregnated corn starch layers with ethanol - n-butanol - water - conc. NH3 8:6:3:3 for organic acids, 2-propanol - formic acid - water 20:1:5 for amino acids, acetone - conc. acetic acid 3:2 for fat-soluble vitamins, n-propanol - benzyl alcohol - water - formic acid - dioxane - benzene 10:27:5:4:10:20 for sugars, and n-butanol - glacial acetic acid - water - benzene 60:40:20:1 for anthocyanins. Detection of organic acids by adding fluorescein (0.02 g) to 100 mL of the mobile phase or by spraying with bromophenol blue reagent; of amino acids with ninhydrin-lutidine reagent; of fat-soluble vitamins with antimony(III)chloride solution in chloroform; and of carbohydrates by spraying with a freshly prepared solution prepared from 4% diphenylamine in ethanol, 4% alanine in ethanol and 85% phosphoric acid, mixed in the ratio 5:5:1. Quantification by densitometry at 254 or 366 nm.

J. Liq. Chrom. & Rel. Technol. 26, 2703-2707 (2003). TLC of vitamin B12 and corrinoid compounds (5-hydroxybenzimidazolyl cyanocobamide, benzimidazolyl cyanocobamide, 7-adenylcyanocobamide) on silica gel with 1-butanol - 2-propanol - water 10:7:10 in the dark at 24°C. The TLC sheet was dried and cut into small pieces; B12 was extracted from the pieces in 80% methanol containing 20 mg/l KCN several times, evaporated to dryness and used as samples for the B12 microbiological assay.

J. Planar Chromatogr. 23, 14-17 (2010). TLC of vitamin C of different concentrations (0.45, 0.50, 0.55, 0.60, and 0.65 mg/mL) and juice (e. g. orange with grapefruit, orange with apple and carrot, “multi-fruit”) mixed with a methanolic solution of DPPH radical (2,2-diphenyl-1-picrylhydrazyl) on silica gel. Images of plates were evaluated using a specific computer software. The equivalence of the results obtained using the procedure described was demonstrated by applying the traditional UV-visible spectrophotometry.

Acta Alimentaria 12, 265-290 (1983). TLC of vitamin C (ascorbic acid and dehydroascorbic acid) on silica with benzene - acetone - pyridine 80:12:8.