Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      131 041
      Coffee oligosaccharides and their role in health and wellness
      S. TRIPATHI, P. MURTHY* (*Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India,

      Food Res. Int. 173, 113288 (2023). Review of production, bio-activity, and the role of coffee oligosaccharides (COS) as a functional food, including TLC and HPTLC separation and characterization techniques for the analysis of COS.

      Classification: 10a
      131 010
      A novel agarase, Gaa16B, isolated from the marine bacterium Gilvimarinus agarilyticus JEA5, and the moisturizing effect of its partial hydrolysis products
      Y. LEE, E. JO, Y.-J. LEE, T.-Y. EOM, Y. GANG, Y.-H. KANG, S. D. MARASINGHE, S. A. HETTIARACHCHI, D.-H. KANG, Chulhong OH* (*Jeju Marine Research Center, Korea Institute of Ocean Science and Technology, Gujwa-eup, Jeju, Korea;

      Marine Drugs 20(1), 2 (2022). Samples were the products of partial vs. complete hydrolysis of agar by rGaa16Bc, a recombinant form of agarase Gaa16B from Gilvimarinus agarilyticus (Cellvibrionaceae) overexpressed in Escherichia coli. D-galactose (G) and its oligomers (neoagarobiose (NA2), neoagarotetraose (NA4), neoagarohexaose (NA6)) were used as standards. TLC on silica gel with n-butanol – acetic acid – water 2:1:1. Visualization by spraying orcinol reagent (50 mg orcine monohydrate in 100 mL acetone and 8 mL sulfuric acid), followed by 10 min heating at 110° C. The observed patterns showed the apparition of NA6 and NA4 among the hydrolytic products already after 20 min reaction, whereas NA4 and NA2 were the main products after over-night complete hydrolysis.

      Classification: 4e, 10a
      130 040
      Partial characterization of novel inulin-like prebiotic fructooligosaccharides of Sechium edule (Jacq.) Sw. (Cucurbitaceae) tuberous roots
      B. BANDYOPADHYAY, V. MANDAL, N. MANDAL* (*Plant and Microbial Physiology and Biochemistry Laboratory, Department of Botany, University of Gour Banga, Malda –732 103, WB, India,

      J. Food. Biochem. 45, e13764 (2021). HPTLC of L-arabinose, D-fructose, D-fucose, D-galactose, D-glucose, D-mannose, D-rhamnose, D-xylose in the roots of Sechium edule on silica gel with chloroform - n-butanol - methanol - water - acetic acid 9:15:10:3:3. Detection by spraying with 5 % sulfuric acid in methanol containing 0.1 % orcinol, followed by heating at 80 °C for 5-10 min.

      Classification: 10a
      130 071
      Insights into β-manno-oligosaccharide uptake and metabolism in Bifidobacterium adolescentis DSMZ 20083 from whole-genome microarray analysis
      P. MARY, P. MONICA, M. KAPOOR* (*Department of Microbiology and Fermentation Technology, CSIR-Central Food Technological Research Institute, Mysuru 570020, India,

      Microbiol. Res. 266, 127215 (2023). HPTLC of β-manno-oligosaccharides mannobiose (1), mannotriose (2), and mannotetrose (3) in the fermented broth of adult-associated Bifidobacterium adolescentis DSMZ 20083 on silica gel with butanol - water - acetic acid 2:1:1 overnight (12-15 h). Detection by spraying with a mixture of 1 mg/mL orcinol, 75 % ethanol and 3.2 % sulfuric acid in water, followed by heating at 100 °C for 10 min. The method was used to determine the ability of adult-associated B. adolescentis DSMZ 20083 to utilize β-manno-oligosaccharides from guar gum, locust bean gum, konjac root, and copra meal generated using GH26 endo-β-mannanase (ManB-1601).

      Classification: 10a
      130 097
      Fucose-containing Abroma augusta mucilage hydrogel as a potential probiotic carrier with prebiotic function
      A. ROY, M. PATRA, S. SARKHEL, S. SENGUPTA, S. SAHA, S. JHA, G. SARKHEL, S. SHRIVASTAVA (*Laboratory of Food Chemistry and Technology, Department of Chemical Engineering, Birla Institute of Technology Mesra, Ranchi, Jharkhand, India,

      Food Chem. 132941 (2022). HPTLC of monosaccharides glucose, galactose and rhamnose in Abroma augusta mucilage on silica gel with acetone - butanol - water 5:4:1. Detection by spraying with orcinol-sulfuric acid solution, followed by heating at 90 °C for 5 min. 


      Classification: 10a
      130 033
      Reagent sequence for planar chromatographic analysis of eight sweeteners in food products approved in the European Union
      Gertrud MORLOCK*, G. SABIR (*Institute of Nutritional Science, and Interdisciplinary Research Centre, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany,

      J. Planar Chromatogr. 35, 273-279 (2022). HPTLC of sweetener saccharin (1), acesulfame-K (2), neohesperidin (3), aspartame (4), stevioside (5), rebaudioside A (6), sucralose (7), and Na-cyclamate (8) in food samples on silica gel with ethyl acetate - methanol - acetic acid 5:1:1. Detection by dipping into the following reagent sequece, followed each by plate heating and image documentation or densitometry: 1) Primuline reagent (100 mg primuline in 20 mL water and 80 mL acetone), followed by solvent evaporation and detection at 366 nm; 2) ninhydrin reagent (0.3 g ninhydrin dissolved in 95 mL isopropyl alcohol and 5 mL glacial acetic acid), followed by heating at 120 °C for 5 min and detection at white light; 3) 2-naphthol sulfuric acid reagent (1 g 2-naphthol dissolved in 90 mL ethanol and 6 mL 50 % sulfuric acid added dropwise), followed by heating at 120 °C for 5 min and detection at white light. Quantification by absorbance measurement at 200 nm for (1), 230 nm for (2), 290 nm for (3), 500 nm for (4) to (7) and 650 nm for (8).  Linearity was between 30 and 600 ng/zone for (5) and (6) and 800 and 1600 ng/zone for (8).   

      Classification: 10a, 23e
      129 030
      High‑performance thin‑layer chromatography method development and validation for quantification of glucuronic acid in gum samples of Sterculia urens Roxb.
      H. SAXENA*, S. PARIHAR, G. PAWAR, V. SAHU (*NWFP Section, SFM and AF Division, Tropical Forest Research Institute, Jabalpur, Madhya Pradesh 482021, India,

      J. Planar Chromatogr. 35, 153-159 (2022). HPTLC of glucuronic acid in gum samples of Sterculia urens on silica gel with 1-propanol - water 7:3. Detection by spraying with napthoresorcinol sulfuric acid reagent, followed by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 580 nm. The hRF value for glucuronic acid was 43. Linearity was between 300 and 700 ng/zone. Interday and intra-day precisions were below 2 % (n=3). Recovery was between 100.4 and 102.3 %.

      Classification: 10a, 11a
      129 002
      Imaging high-performance thin-layer chromatography as powerful tool to visualize metabolite profiles of eight Bacillus candidates upon cultivation and growth behavior
      S. KRUSE, F. PIERRE, Gertrud E. MORLOCK* (*Institute of Nutritional Science, and Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Giessen, Germany;

      J Chromatogr A, 1640, 461929 (2021). Study of the impact of different strains, culture media and parameters (temperature, time, rotational speed, and glucide and amino-acid supply) on the metabolite profile of bacteria. Samples were cultivation broths of Bacillus subtilis, B. licheniformis, B. pumilus and B. amyloliquefaciens, as well as their respective supernatant liquid-liquid extracts (apolar solvents only or QuEChERS method with acetonitrile and MgSO4 – NaCl mixture 4:1). HPTLC on silica gel (normal phase and RP-18), either as bands (for small volumes of extracts) or as areas for supernatants and bigger volumes of extracts. Extract areas were focused with a three-step procedure (up to 20mm with acetone, and twice with methanol); unextracted supernatants were focused twice with methanol and once with tetrahydrofuran, but the application zone of the plate had to be cut before development, due to the high matrix load. Development with ethyl acetate – methanol – water at different ratios after activation of the plate surface with magnesium chloride (33% relative humidity), evaluation in white light and UV. Detection of antibacterial compounds with Aliivibrio fischeri bioassay. Derivatization with primuline (for lipophilic substances) and diphenylamine aniline sulfuric acid reagent (for saccharides). This method allowed a fast comparison: A) of the patterns of the different strains (presence /absence and intensity of detected or antibacterial bands); B) of cultivation parameters: the number of metabolites increased with time, rotational speed (oxygen level), and at 37°C (vs. 30°C), whereas a minimal medium allowed the detection of more metabolites, due to the lower matrix load; C) of the impact of the extraction parameters: choice of the solvents (QuEChERS method had no advantage here), solvent – supernatant ratio (1:3 showed richer patterns than 1:1); D) of the HPTLC parameters used (better separation and resolution with normal phase vs. RP18 layers).

      Classification: 3a, 10a, 11c, 27