E-Journal of Chemistry 7(51), 5559-5565 (2010). TLC of sucralose in commercially available tabletop sweeteners, dietetic sweets and soft drinks on silica gel with chloroform - methanol - toluene 10:7:3 (system 1) and chloroform - ethanol - benzene 5:3:2 (system 2). The hRf value of sucralose was 62 with system 1 and 45 with system 2. Detection by dipping in rhodamine-sulphuric acid reagent, followed by heating at 120 °C for 3 min. The band corresponding to sucralose appears as olive-green band with ?max at 456 nm. The fluorescence property of the sucralose derivative can be used for quantitative analysis (?max 366 nm). The method is highly reproducible as other carbohydrates and artificial sweeteners don’t produce a fluorescent olive-green color with this reagent. The method was applied to cola drinks, lemon juices, sugar free sweets, and tabletop sweeteners with excellent results. The LOD was 5-7 ng/band and linearity was in the range of 40-250 ng/band for both methods.

J. Planar Chromatogr. 30, 170-174 (2017). HPTLC-overpressured layer chromatography of monosaccharides (glucose, xylose, rhamnose, and arabinose) as substrates and sugar alcohols (xylitol, sorbitol and arabitol) as metabolites, to follow the fermentation of the yeast Candida tropicalis DSM 7524 on silica gel with acetonitrile – acetic acid – water 63:33:5. Detection by dipping for 9 s into a reagent containing 5 mL of solution I (2 % basic acetate of lead (IV) in glacial acetic acid) and 5 mL of solution II (0.2 % 2,7-dichlorofluorescein in 100 mL ethanol) made up to 200 mL with toluene, followed by heating at 105 °C for 3 min. Quantitative determination by absorbance measurement at 366 nm.

J. Chromatogr. 373, 81-110 (1986). Review on TLC determination of mono- and disaccharides with more than 70 references.

Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 265-271 (1991). HPTLC of xylose, 3-o-methylglucose and rhamnose in urine with arabinose as internal marker on silica with ethyl acetate - pyridine - acetic acid - water 75:15:10:10. Densitometry after three consecutive runs. Visualization by dipping into aminobenzoic acid reagent. Densitometry by absorbance at 400 nm. CV < 2.7%.

Chromatographia 45, 195-198 (1997). 2D-TLC of amino acids, sugars, oligosaccharides, and organic acids on silica and cellulose with different solvents. Detection with suitable spray reagents. Application of the technique for diagnosis of inherited metabolic diseases with some pathological examples.

J. Planar Chromatogr. 16, 52-57 (2003). HPTLC of fructooligosaccharides on NH2-silica gel with acetonitrile - water - phosphate buffer 16:3:2 and on silica gel, pretreated with 0.02% sodium acetate or chloroform - methanol 1:2, with chloroform - dichloromethane - methanol - water 35:15:35:6, acetonitrile - water 6:1 and butanol - ethanol - water 5:3:2. Detection by immersion for 5 s in aniline - diphenylamine - phosphoric acid dipping solution. Visualization by heating at 120°C for 20 min. Densitometry at 370 nm. New rapid and precise procedure.