Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

      130 147
      Globotriaosylceramide-related biomarkers of Fabry disease identified in plasma by high-performance thin-layer chromatography – densitometry – mass spectrometry
      C. JARNE, L. MEMBRADO, M. SAVIRÓN, J. VELA, J. ORDUNA, R. GARRIGA, J. GALBÁN, V. L. CEBOLLA* (*Institute of Carbon Chemistry, Spanish National Research Council (CSIC), Saragossa, Spain;

      J Chromatogr A 1638, 461895 (2021). Samples were sphingolipid-rich fractions of unproteinated blood plasma from healthy humans or from Fabry’s disease patients, as well as standards of sphingomyelin (SM) and of globotriaosylceramides (Gb3 = ceramide trihexosides), and related compounds (lyso-ceramide trihexosides, lactosyl ceramide, glucosyl ceramide). HPTLC on silica gel (Lichrosphere with spherical particles) by automated multiple development with a 9-step gradient, starting with pure methanol and ending with dichloromethane – methanol 9:1. Visualization and densitometry under UV 190 nm. Derivatization for Gb3 and derivatives (but not for SM) by immersion into orcinol solution (0.2 %, with sulfuric acid 10 %), followed by 15 min heating at 100 °C and by densitometry under visible light 550 nm. Bands of interest were directly eluted with methanol from underivatized plates into an ion-trap MS, through the oval head of a TLC-MS interface (with stainless steel frit to remove silica gel particles). Two different ionization processes were used: (A) electrospray ionization (ESI, capillary voltage 4 kV, endplate offset voltage -0.5 kV, nebulizer pressure 40 psi, drying gas 9 mL/min at 350 °C); (B) atmospheric pressure chemical ionization (APCI, capillary voltage 2–3 kV, current intensity 4.5 µA, nebulizer pressure 45 psi, drying gas 5 mL/min at 350 °C; vaporization at 450 °C). Full MS spectra were recorded up to m/z 1500 in positive ion mode. The relative ion intensities were used to quantify the detected species. Previous to this study, the precision of the elution head positioning was tested on Gb3 standard zones, comparing 3 positions for analyte elution: from the centre and from each higher or lower side of the band. The same main m/z peaks were observed in the 3 positions, but in different proportions. This was explained by the presence of coeluting Gb3 subclasses (the ceramide moiety CM being either saturated, mono-unsaturated fatty acyl with a slightly higher migration distance, or polar hydroxyl fatty acyl with the opposite effect on migration) and of coeluting Gb3 isoforms (the hexoside moiety consisting of glucose and/or galactose units). This resulted in the broadening and partial splitting of the standard band. In the plasma samples, 19 molecular species of Gb3 were identified (depending on the CM, the sugar isoforms being undistinguishable by MS): 5 with a saturated CM, 7 with two additional double bonds on the CM, 7 with a methylated CM. In case of Fabry’s disease, most Gb3 species with saturated CM were highly increased, whereas other species were decreased.

      Classification: 4e, 11c, 11e, 32f
      130 057
      Sensitive and selective bioscreening of the most commonly used coronavirus disease drug, favipiravir, and its co-administered therapeutic, meropenem, in human plasma
      R. ABDELFATAH, E. ABDELALEEM, E. ABDELMOMEN*, R. ABDELMOETY, A. EMAM (*Faculty of Pharmacy, Nahda University (NUB), New Beni-Suef City, Egypt,

      J. Sep. Sci. 45, 3800-3810 (2022). HPTLC of favipiravir (1) and meropenem (2) in human plasma on silica gel with ethyl acetate - methanol - water - formic acid 50:40:15:3. Quantitative determination by absorbance measurement at 300 nm for (1) and (2). The hRF values for (1) and (2) were 34 and 10, respectively. Linearity was between 0.1 and 20 µg/mL for (1) and 10 and 60 µg/mL for (2). Inter-day and intra-day precisions were below 5 % (n=3). The LOQ were 0.1 and 10 µg/mL for (1) and (2), respectively. Mean recovery was 99.7 % for (1) and 98.0 % for (2).

      Classification: 32f
      126 054
      Semi-quantitative analysis of drugs of abuse in human urine by end-point dilution flow immunochromatographic assay
      S. BADAWY (National Center for Clinical and Environmental Toxicology, NECTR, Faculty of Medicine, Cairo University, Giza, Egypt,

      J. Planar Chromatogr. 33, 419-425 (2020). Immunochromatography of cannabinoids (1) and opiates (2) in human urine using a test cassette device consisting of five parts, including a plastic backing, sample pad, conjugate pad, absorbent pad, and nitrocellulose (NC) membrane. Visual LOD was 50 ng/mL for (1) and 300 mg/mL for (2). Recovery was between 98 and 111 % for (1) and 101 and 110 % for (2).

      Classification: 32f
      101 064
      Bio-activity based analysis of irradiated sunscreens using HPTLC and in situ detection with Vibrio fischeri
      U. HAURI, Vera BAUMGARTNER. CH. HOHL* (*Kantonales Laboratorium Basel-Stadt, Non Food, P.O. Box, 4012 Basel, Switzerland, christopher.

      CBS 100, 2-5 (2008). HPTLC of photodegraded UV filters and sunscreen on silica gel LiChrospher prewashed with methanol. AMD 2 development of UV filter standards photodegradation products with diisopropylether - n-hexane in 6 steps over 50 mm without preconditioning, and of sunscreen samples photodegradation products with t-butylmethylether - n-hexane in 7 steps over 50 mm with preconditioning, followed by drying at 120 °C for 30 min. Detection at 254 and 366 nm, followed by biodetection via dipping the plate in a Vibrio fischeri solution for 1 s and evaluation with the Bioluminizer (exposure time 55 s). Densitometric evaluation by multi-wavelength scan at 200-400 nm.

      Classification: 32f
      55 026
      HPTLC separation of PGF2, PGE1, PGE2, PGA1, prostaglandins and quantitative determination in situ by induced fluorescence

      J. High Resol. Chromatogr. 7, 593-595 (1984). HPTLC of the title compounds on silica with ethanol - ether - ethyl acetate - chloroform 12:33:22:33. Detection by spraying with 5 % cupric acetate in 15 % aqueous phosphoric acid and heating at 110 °C for 10 minutes. Fluorometry at 366 nm. Sensitivity 1-10 ng.

      Classification: 11b, 32f
      58 153
      (Determination of theophylline in plasma by thin-layer chromatography
      B. LI, L. TONG, B. ZOU. Acta Pharmaceutica Sinica (Yaoxue Xueboa) 20, 398-400 (1985). (Chinese)

      TLC on silica with chloroform - ethanol 15:1. Determination by densitometry at 274 nm. Detection limit 100 ng.

      Classification: 32f
      63 181
      Field-adapted method for high-performance thin-layer chromatographic detection and estimation of chloroqine in finger-stick blood
      D. MOUNT, L.C. PATCHEN, F.C. CHURCHIL*, (*Cont. Tech. Branch, Div. Parasitic Diseases, Cent. Infect. Diseases, Cent. Desease Cont., Pub. Heal. Serv., U. S. Dep. & Human Serv., Atlanta, GA 10333, USA)

      J. Chromatogr. 428, 196-202 (1988). HPTLC of chloroquine on silica with 4% n-butylamine in methyl tert. -butyl ether. Detection under UV. Estimation by comparison of the spot fluorescence intensity with standards. Comparison of the results with those from HPLC.

      Classification: 32f
      67 208
      Microanalysis of human serum & blood cell membrane lipids by HPPC
      J. PICK, (National Inst. of Haematology and blood transfusion, P.O. Box 44, Budapest, H-1502 Hungary)

      Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 247-256 (1991). HPTLC of gangliosides on silica using OPLC technique with a gradient composed from three isocratic steps. 1) For ganglioside mapping (OPLC) with a) chloroform - methanol 95:5, b) chloroform - methanol - water 65:25:3, c) chloroform - methanol - 0.25 % KCl/H2O 55:36:9. Visualization with resorcinol-HCl reagent and heating. 2) For wide lipid mapping (multi development) with a) propanol - methyl acetate - chloroform - methanol - ethanol 43 mM KCl 23:23:29:11:10:12, b) hexane - methyl acetate - toluene - chloroform 300:54:25:25, c) carbon tetrachloride. Visualization by spraying with phosphomolibdic acid or CuSO4; densitometry by absorbance at 525, 540 and 546 nm.

      Classification: 32f